226 Results for: "pH Colored Solutions"

Supplier: Electron Microscopy Sciences

Citifluor™ mountant media containing antifadents solutions reduce the photo-bleaching or fading of the fluorescence of dyes used for labeling biological species.

Supplier: Electron Microscopy Sciences

Premixed, prebuffered fixatives, that are pH adjusted for immediate laboratory use.

Supplier: Electron Microscopy Sciences

Premixed, prebuffered fixatives, that are pH adjusted for immediate laboratory use. They are freshly prepared upon receipt of order to ensure maximum shelf life. The fixatives are filled in 20 mL glass vials (10 mL/vial). This allows for the immediate fixation of your specimens without costly and time consuming transfer associated with ampoule contained fixatives.

Supplier: Enzo Life Sciences

In order to perform immunostaining, tissue specimens should be preserved in an appropriate fixative.

Supplier: MP Biomedicals

Aprotinin is a natural inhibitor of trypsin, chymotrypsin and plasmin. It is found in bovine lymph nodes, lung, parotid gland, spleen, liver, pancreas, seminal vesicles, thyroid gland, kidney, mucous membranes of the trachea and esophagus, ovaries, heart, posterior pituitary and cartilage.A serine protease inhibitor which is reactive against trypsin, chymotrypsin, plasmatic and glandular kininogenases, plasmin, kallkrein, urokinase, clotting factor XIIa, protein C, proteinases of the complement system, and leukocyte and tissue proteinases. It does not inhibit thrombin. Aprotinin works by blocking the active sites of enzymes. Binding is reversible with most aprotinin-protease complexes dissociating at pH > 10 or < 3.
Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.

Supplier: MP Biomedicals

3,3',5,5'-Tetramethylbenzidine is suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620 to 650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases. It is useful in gel staining procedure for low levels of home-associated peroxidases and for enzyme immunoassay of horseradish peroxidase.

Supplier: MP Biomedicals

BES is a zwitterionic buffer that is used in biochemistry and molecular biology research in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies.

Supplier: Epredia

Ensure crisp nuclear detail with the ready-to-use Epredia™ Signature Series™ Bluing Reagent.

Supplier: MP Biomedicals

Pure Canavalia ensiformis lectin (Con A) from Jackbean.

Supplier: Adipogen

A sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues. Used to characterize reactive thiol groups and photometric determination of thiols and for measuring low-molecular mass thiols such as glutathione in both pure solutions and biological samples, such as blood. It can also measure the number of thiol groups on proteins. Through reaction with aliphatic thiol groups a mixed disulfide of protein thiol and one mole of 2-nitro-5-thiobenzoate per mole of protein sulfhydryl group is being formed. DTNB has little absorbance. Reaction with -SH groups on proteins (from any solvent accessible Cys) under mild alkaline conditions (pH 7-8) produces the 2-nitro-5-thiobenzoate anion, which gives an intense yellow color with an absorption maximum at 409.5nm (Extinction coefficient: 14150 M-1*cm-1). Sensitive to various buffer ions, therefore, the extinction coefficient used to calculate the number of sulfhydryl groups must be matched to the reaction conditions. In case the thiol groups are in disulfide bonds, they must be reduced under anaerobic conditions prior to reaction with DTNB.

Supplier: Epredia

Epredia™ Dewax and Heat Induced Epitope Retrieval (HIER) H Buffer simultaneously automate deparaffinization, rehydration and epitope retrieval in the Epredia™ PT Module.

Supplier: MP Biomedicals

Dextran sulfate is a polyanionic derivative of dextran produced by esterification of Dextran with chlorosulphonic acid. Dextran sulfate sodium (DSS) is the sodium salt which is a white to off-white powder freely soluble in water and salt solutions to form a stable, clear solution. The high purity and reproducible quality enables its application in pharmaceuticals and biotechnology industry.

Supplier: MP Biomedicals

Choline chloride is an organic compound and a quaternary ammonium salt. It is a weak acid. Choline chloride is the salt of the naturally occurring choline, the pre-stage of the neurotransmitter acetylcholine, which is important for mnemonic and thought-processes. Choline occurs naturally in fungi, hop and kingcups and as integral part of lecithin. Choline chloride is a common food additive in animal husbandry
The enzymatic activities of butyrylcholinesterase (BChE) and paraoxonase 1 (PON1), two serum enzymes synthesized by the liver and related with inflammation, were decreased in a sepsis animal model injected with LPS. Choline chloride administered intravenously at 20 mg/kg body weight prevents the LPS-mediated decreases in the activities of these two enzymes.
Choline chloride is used as a source of methyl groups for methyl-transfer reactions.
Physical Appearance: Hard, White Crystalline Powder
pH: Neutral to Litmus
Vapor Pressure: 10 hPa at 20 °C (Lit.)
Partition Coefficient: n octanol/water (logKow): -3.77 at 25 °C (lit.)
Solubility: Soluble in water: (10% aq soln) Clear, Colorless Solution

Supplier: MP Biomedicals

Sodium phosphate is a reagent with very high buffering capacity that is widely used in molecular biology, biochemistry, and chromatography. Sodium phosphate occurs in several forms: monobasic (NaH2PO4), dibasic (Na2HPO4), and tribasic (Na3PO4). Most neutral sodium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH.
Sodium phosphate monobasic, Monohydrate is used as sequestrant, emulsifier and buffer in foods; as mordant in dyeing; for weighting silk in tanning; in manufacture of enamels, ceramics, detergents, boiler compounds; as fireproofing agent; in soldering and brazing instead of borax; as reagent and buffer in analytical chemistry; cathartic; laxative. A table for preparation of 0.1 M sodium phosphate buffer at 25 °C using various proportions of sodium phosphate monobasic and sodium phosphate dibasic has been published. A study of the effect of freeze-thaw storage cycles on proteins in sodium phosphate and potassium phosphate buffer solutions has been reported. The effect of 5 mM sodium phosphate on the efficacy of electrospray ionization (ESI) ion mobility spectrometry (IMS) analysis has been evaluated. A protocol for the purification of pyrogen-free mouse IgG1 monoclonal antibodies which uses 10 mM sodium phosphate (pH 7.4) has been published. An ion-pairing HPLC method for the analysis of 5-aminosalicylic acid has been reported. A TLC method for separation of nucleotide sugars in the study of glycosyltransferase activity has been published.
Room Temperature, Desiccate

Supplier: MP Biomedicals

Arachidonic Acid is an essential fatty acid. Occurs in liver, brain, glandular organs, and depot fats of animals, in small amounts in human depot fats, and is a constituent of animal phosphatides.
Arachidonic Acid is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes. Arachidonic acid plays a key role in cellular regulation and is controlled through multiple interconnected pathways.
Arachidonic acid (AA) is an unsaturated ω6 fatty acid constituent of the phospholipids of cell membranes. Phospholipase A2 releases AA from the membrane phospholipids in response to inflammation. AA is subsequently metabolized to prostaglandins and thromboxanes by at least two cyclooxygenase (COX) isoforms, to leukotrienes and lipoxins by lipoxygenases, and to epoxyeicosatrienoic acids via cytochrome p450-catalyzed metabolism. AA and its metabolites play important roles in a variety of biological processes, including signal transduction, smooth muscle contraction, chemotaxis, cell proliferation and differentiation, and apoptosis. AA has been demonstrated to bind to the a subunit of G protein and inhibit the activity of Ras GTPase-activating proteins (GAPs). Cellular uptake of AA is energy dependent and involves protein-facilitated transport across the plasma membrane.
If ethanol is undesirable, arachidonic acid may be dissolved in acetonitrile, DMF, or DMSO. Simply evaporate the ethanol under a gentle stream of nitrogen (be certain not to evaporate the material to dryness) and redissolve the arachidonic acid in the solvent of choice.Just prior to use, make dilutions of the stock solution into aqueous buffer or isotonic saline to bring the arachidonic acid to the desired concentration. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiologic effects at low concentrations. A control using the solvent in the absence of the prostaglandin will address this potential variable. We do not recommend storing the aqueous solution for more than one day. It is difficult to obtain aqueous solutions of arachidonic acid directly. However, an organic solvent free solution of arachidonic acid can be prepared using concentrated basic buffers (pH > 8.0 and ionic strength not less than 0.1 M). Add 400 μL of cold buffer (0 °C) per mg of arachidonic acid and agitate vigorously and/or ultrasonicate.

Supplier: MP Biomedicals

Folic acid, also known as folate, is a B vitamin that can be found in a variety of fruits and vegetables. It can also be chemically synthesized. Folate, a watersoluble vitamin, helps the body form red blood cells and aids in the formation of genetic material within every body cell. This product exhibits metal binding properties. Hematopoietic vitamin present, free or combined with one or more additional molecules of L-(+)-glutamic acid, in liver, kidney, mushrooms, spinach, yeast, green leaves, and grasses.
Folic acid (Vitamin B9 and folate) is essential to numerous bodily functions. The human body needs folate to synthesize DNA, repair DNA, and methylate DNA as well as to act as a cofactor in certain biological reactions. It is especially important in aiding rapid cell division and growth, such as in infancy and pregnancy.
Folic acid (FA) and dihydrofolic acid (FAH2) are substrates of dihydrofolate reductase(s) which reduce them to tetrahydrofolate (THF), which in turn supports ‘one carbon’ transfer. Tetrahydrofolates are required for de novo synthesis of purines, thymidylic acid and various amino acids and for post-translational methylation (epigenetics).
Very slightly soluble in cold water (0.0016 mg/mL at 25 °C), soluble to about 1% in boiling water. Slightly soluble in methanol, appreciably less soluble in ethanol and butanol. Insoluble in acetone, chloroform, ether, benzene. Relatively soluble in acetic acid, phenol, pyridine, solutions of alkali hydroxides and carbonates. Soluble in hot dilute HCl and H2SO4.

Supplier: MP Biomedicals

Cholic acid is a biochemical solubilizing agent (a non-ionic, non-denaturing detergent). Occurs inconjugation with glycine or taurine in bile of most vertebrates.

Supplier: Electron Microscopy Sciences

Series of pH indicator papers where the indicator and color scale are combined on a single strip.

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

High quality buffer solutions ensure accurate pH measurements time after time.

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

Accurate to within ±0.01 pH at 25 °C (±0.02 for pH 9 and 9.18).

Supplier: Ward's Science

Easily determine pH with the universal indicator chart.

Supplier: Micro Essential

One drop of the indicator solution in a 4ml sample will provide bright, easy-to-read colors

Supplier: Avantor

Simple Yet Effective Demonstration

Supplier: Mettler Toledo

NIST-traceable buffer for pH electrode calibration and verification.

Supplier: Electron Microscopy Sciences

In contrast to conventional indicator papers, the indicator dyes of pH-Fix sticks are substantially bound to the cellulose fibers

Supplier: Ricca Chemical

pH Buffer Reference Standard Solution for Calibration, pH 4.00 0.01 at 25°C.

Supplier: VWR International

A ready-to-use kit that fits into your lab coat pocket.

Supplier: Thermo Fisher Scientific

Hold 5 gallons (19L) of pH buffer in color-coded Orion™ pH Buffer Cubitainers™

Supplier: VWR International

A ready-to-use kit that fits into your lab coat pocket.

Supplier: MilliporeSigma

Roll with color scale Ph Eur.