Maxwell RSC Premier Service Agreement, 1 each, Promega
Supplier: Promega Corporation
Maxwell RSC Standard Warranty.
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Dextran sulfate sodium salt, M.W. ∼ 8,000, white powder, Reagent Grade
Supplier: MP Biomedicals
Dextran sulfate is a polyanionic derivative of dextran produced by esterification of Dextran with chlorosulphonic acid. The sulfur content is approximately 17% which corresponds to an average of 1.9 sulfate groups per glucosyl residue of the dextran molecule. Dextran sulfate sodium (DSS) is the sodium salt which is a white to off-white powder freely soluble in water and salt solutions to form a stable, clear solution. The high purity and reproducible quality enables its application in pharmaceuticals and biotechnology industry.
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HaloTag Control Vector, 20microg, Promega
Supplier: Promega Corporation
The HaloTag Mammalian Pull-Down Systems capture and purify intracellular protein complexes for correlative cellular localization and real-time imaging studies with the same genetic construct for further understanding of overall protein function.
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Maxwell RSC Premier Warranty Upgrade, 1 each, Promega
Supplier: Promega Corporation
Maxwell RSC Standard Warranty.
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Pierce™ Surfact-Amps™ Detergent Sampler, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Surfact-Amps Detergent Sampler is a convenient collection of various Thermo Scientific Surfact-Amps Detergent Solutions and selected other useful detergents for protein research methods.
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Pierce™ Zinc Reversible Stain Kit, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce Zinc Reversible Stain Kit provides rapid and highly sensitive detection of protein in polyacrylamide gels immediately after electrophoresis as well as erasability for subsequent transfer or band extraction.
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Dithiothreitol (DTT, Cleland's reagent) ≥99.0%, white powder
Supplier: MP Biomedicals
DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.
DTT has been used:
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Anti-IgG Rat Mouse Monoclonal Antibody (DL800) [clone: eB144]
Supplier: Rockland Immunochemical
Mouse IgG TrueBlot® is a unique DyLight™ 800 conjugated anti-mouse IgG immunoblotting (second step) reagent. Mouse IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Fluorescent Western Blot data with Mouse IgG TrueBlot®, simply substitute the conventional DL800 anti-mouse IgG blotting reagent with Fluorescent Mouse TrueBlot® Antibody DyLight™ 800 and follow the prescribed protocol for sample preparation and immunoblotting.
Mouse IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Fluorescent Mouse TrueBlot® Antibody DyLight™ 800 may also be used for detection in immunoassays that do not employ immunoprecipitation.
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STORAGE BAG WESTERN BLOT 13.3 CM X 20 CM
Supplier: Li-Cor
STORAGE BAG WESTERN BLOT 13.3 CM X 20 CM
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3M™ Zeta Plus™ DEL Series Filter Cartridge
Supplier: 3M Healthcare
Zeta Plus™ BC Series Filter Capsule with DEL Series Media incorporates lipid-specific adsorbents that help reduce lipids, surfactants and other biological contaminants from human blood plasma, serum-based products, recombinant proteins and other biological fluids.
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3M™ High Flow Series System for Ice Applications Valve-in-Head Design, Model ICE140-S, 5616203
Supplier: 3M Healthcare
3M™ High Flow Series System for Ice Applications Valve-in-Head Design, Model ICE140-S provides consistent high quality water by reducing particulate, chlorine taste and odor, cyst*, bacteria1, and scale**. The Sanitary Quick Change (SQC) design allows for easy cartridge change-outs.