"Surmodics"

Supplier: Surmodics

The StabilBlot™ Blocker Family offers a range of options, both protein-based and synthetic-based, to block non-specific binding as well as enhance sensitivity when used as a blocker and antibody diluent.

Supplier: Surmodics

StabilZyme® HRP, StabilZyme® Protein-Free and StabilZyme® NOBLE stabilizers provide the assay developer a complete set of tools to stabilize different HRP conjugates for long term, in-solution stability.

Supplier: Surmodics

StabilCoat® Immunoassay Stabilizer 5X concentrate, effectively preserves the conformation and activity of dried proteins while simultaneously blocking the surface to reduce non-specific binding. The product can be used to stabilize antibodies, antigens or enzymes on an assortment of immunoassay components including polystyrene plates, tubes, glass, membranes, and filter paper.

Supplier: Surmodics

StabilZyme® HRP, StabilZyme® Protein-Free and StabilZyme® NOBLE stabilizers provide the assay developer a complete set of tools to stabilize different HRP conjugates for long term, in-solution stability.

Supplier: Surmodics

StabilGuard® Choice Stabilizer protects the activity of proteins while simultaneously blocking nonspecific binding sites on microspheres. In microarray applications, the conformation and activity of proteins are effectively preserved in a dried state when stored with StabilGuard Choice stabilizer. The product ultimately increases signal and reduces background to improve the sensitivity of assays.

Supplier: Surmodics

DAB (3,3’-diaminobenzidine) is oxidized in the presence of peroxidase and hydrogen peroxide resulting in the deposition of a brown, alcohol-insoluble precipitate at the site of enzymatic activity.

Supplier: Surmodics

StabilZyme® HRP, StabilZyme® Protein-Free and StabilZyme® NOBLE stabilizers provide the assay developer a complete set of tools to stabilize different HRP conjugates for long term, in-solution stability.

Supplier: Surmodics

Chemiluminescent substrates provide a higher degree of sensitivity than other substrates to enable lower limits of detection to be achieved in ELISA and membrane assays. The increase in sensitivity requires more vigilance during the optimization process through the proper selection of the dioxetane substrate, antibody/antigen titers and blockers. Surmodics provides multiple 1-component AP chemiluminescent substrates with various intensities and wavelength emissions to allow the assay developer to choose the appropropriate substrate for the application.

Supplier: Surmodics

The one component 3,3’,5,5’-tetramethylbenzidine (TMB) substrate is supplied in a ready-to-use solution. Peroxidase reacts with the TMB substrate solution to produce a soluble blue reaction product that can be read at 370 nm or 620-650 nm for ELISA applications. Stop solutions can be added to prevent further color development and reading the absorbance values at the desired wavelengths.

Supplier: Surmodics

Absorbance is read in the blue spectral range (650 nm).

Supplier: Surmodics

The stop solution provides minimial signal drift compared to sulfuric or hydrochloric acid. Absorbance is read in the yellow spectral range (450 nm) and can be read up to 3 hours after the TMB reaction has been stopped using the Nova-Stop Solution.

Supplier: Surmodics

The one component 3,3’,5,5’-tetramethylbenzidine (TMB) substrate is supplied in a ready-to-use solution. Peroxidase reacts with the TMB substrate solution to produce a soluble blue reaction product that can be read at 370 nm or 620-650 nm for ELISA applications. Stop solutions can be added to prevent further color development and reading the absorbance values at the desired wavelengths.

Supplier: Surmodics

The one component 3,3’,5,5’-tetramethylbenzidine (TMB) substrate is supplied in a ready-to-use solution for membrane applications. A highly intense staining pattern increases the signal-to-noise ratios for improved sensitivity in western blot, dot blots and line assays.

Supplier: Surmodics

BCIP/NBT Purple One Component AP Membrane Substrate contains 5-bromo-4-chloro-3-indolylphosphate and nitroblue tetrazolium for use in immunblotting or other membrane applications.

Supplier: Surmodics

The one component 3,3’,5,5’-tetramethylbenzidine (TMB) substrate is supplied in a ready-to-use solution. Peroxidase reacts with the TMB substrate solution to produce a soluble blue reaction product that can be read at 370 nm or 620-650 nm for ELISA applications. Stop solutions can be added to prevent further color development and reading the absorbance values at the desired wavelengths.

Supplier: Surmodics

The one component 3,3’,5,5’-tetramethylbenzidine (TMB) substrate is supplied in a ready-to-use solution for membrane applications. The staining pattern provides a dark blue precipitate with low background to increase the signal-to-noise ratios in western blot, dot blots and line assays.