3039 Results for: "Southern Biotechnology"

Supplier: Southern Biotechnology

Mouse IgG1-Alexa Fluor 488 for use as a control in flow cytometry and immunohistochemistry / immunocytochemistry assays.

Supplier: Southern Biotechnology

Mouse IgG1-HRP for use as a control in immunohistochemistry / immunocytochemistry assays.

Supplier: Southern Biotechnology

Goat Anti-Mouse IgG2b-Biotin antibody for use in ELISA assays.

Supplier: Southern Biotechnology

AP Anti-Mouse IgG2b antibody for use in ELISA,, immunocytochemistry, and western blotting assays.

Supplier: Southern Biotechnology

Goat Anti-Mouse IgG2a-HRP antibody with minimal reactivity to human, bovine, and horse serum proteins for use in ELISA assays.

Supplier: Southern Biotechnology

Purified Goat Anti-Mouse IgG2b antibody for use in ELISA assays.

Supplier: Southern Biotechnology

Purified Anti-Mouse IgG3 antibody for use in ELISA and flow cytometry assays.

Supplier: Southern Biotechnology

In the mouse, CD8 exists in two forms – (i) a CD8 heterodimer composed of an α chain (CD8α) and a β chain (CD8β); and (ii) a homodimer of two α chains. The heterodimer is found on the surface of essentially all thymocytes and the “suppressor/cytotoxic” subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8α without CD8β. It has been suggested that CD8+β- T cells mature extrathymically, while development of the CD8α+β+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8α+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.

Supplier: Southern Biotechnology

Goat Anti-Bovine Serum Albumin-Biotin antibody with minimal reactivity human serum albumin for use in ELISA assays.

Supplier: Southern Biotechnology

Purified Goat Anti-Human IgA antibody with minimal reactivity to mouse, bovine, and horse serum proteins for use in ELISA assays.

Supplier: Southern Biotechnology

Goat Anti-Human Kappa-PE antibody with minimal reactivity to mouse proteins for use in flow cytometry assays.

Supplier: Southern Biotechnology

Porcine CD4 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. CD4 is an accessory molecule in the recognition of foreign antigens in association with MHC Class II molecules by T lymphocytes.

Supplier: Southern Biotechnology

Goat F(ab')2 Anti-Human Lambda-Alexa Fluor 488 antibody with minimal reactivity to mouse proteins for use in flow cytometry assays.

Supplier: Southern Biotechnology

Purified Goat Anti-Human IgG Fc antibody with minimal reactivity to mouse, bovine, and horse serum proteins for use in ELISA assays.

Supplier: Southern Biotechnology

FITC Anti-Monkey IgG for use in flow cytometry and western blotting assays.

Supplier: Southern Biotechnology

Mouse Anti-Rabbit IgG(H+L)-Biotin antibody with minimal reactivity to mouse, human, goat, and sheep serum proteins for use in ELISA assays.

Supplier: Southern Biotechnology

Goat Anti-Human Kappa-Alexa Fluor 555 antibody for use in immunohistochemistry / immunocytochemistry and western blotting assays.

Supplier: Southern Biotechnology

Goat Anti-Human IgG Fc-HRP antibody with minimal reactivity to mouse, bovine, and horse serum proteins for use in ELISA assays.

Supplier: Southern Biotechnology

CD4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested. CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors. The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells. In vivo and in vitro treatment with the GK1.5 monoclonal antibody effectively depletes CD4+ cells. GK1.5 also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.

Supplier: Southern Biotechnology

Biotin Anti-Mouse IgG1 antibody for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, and western blotting assays.

Supplier: Southern Biotechnology

Alexa Fluor 488 Anti-Mouse IgG1 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blotting assays.

Supplier: Southern Biotechnology

HRP Anti-Human Type I Collagen antibody for use in ELISA and immunohistochemistry assays.

Supplier: Southern Biotechnology

CD4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested. CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors. The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells. In vivo and in vitro treatment with the GK1.5 monoclonal antibody effectively depletes CD4+ cells. GK1.5 also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.

Supplier: Southern Biotechnology

CD11b, the integrin αM subunit, combines with CD18, the integrin β2 subunit, to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1/CD54. Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 expression is rapidly up-regulated on neutrophils after activation and in the same time period that CD62L is shed from the cell surface. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils. Cross-reaction of M1/70 to human CD11b has been observed on monocytes, polymorphonuclear leukocytes, and NK cells.

Supplier: Southern Biotechnology

The monoclonal antibody DX5 reacts with CD49b, also known as very late antigen-2 (VLA-2) and α2 integrin. The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR). Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes. NK cells cultured in the presence of IL-2 progressively lose reactivity with DX5 as a consequence of cellular proliferation. Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. The monoclonal antibody DX5 has not been demonstrated to have activating or blocking activity.

Supplier: Southern Biotechnology

CD90 (Thy-1) is a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily consisting of a single V-set domain. The Thy-1.2 alloantigen is expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T cells of most mouse strains. It is also expressed in the brain and at varying levels on other non-lymphoid tissues. The 30-H12 monoclonal antibody does not cross react with mouse and rat strains bearing the Thy-1.1 alloantigen (e.g., AKR/J, PL).

Supplier: Southern Biotechnology

CD103 is a member of the integrin series of adhesion molecules. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis, and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. The 2E7 monoclonal antibody exhibits antigen immunoprecipitation patterns similar if not identical to the rat anti-mouse CD103 monoclonal antibody M290. 2E7 is reported to have signal-inducing activity in a redirected lysis assay and to costimulate IEL and CD8+ lymph node cells in conjunction with anti-TCR in vitro which runs counter to the usual inhibition of CTL by anti-integrins. This costimulatory activity is also shared by the monoclonal antibody M290. The property of costimulation is not unique to the mouse since the combination of anti-human αIEL plus anti-TCR will produce similar proliferation in human cells with the exception that CD8+ cells are not stimulated.

Supplier: Southern Biotechnology

CD11b (integrin αM subunit) combines with CD18 (integrin β2 subunit) to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54). Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils.

Supplier: Southern Biotechnology

Goat F(ab’)2 Anti-Mouse IgG1-Alexa Fluor 488 antibody with minimal reactivity to human proteins for use in flow cytometry and immunohistochemistry / immunocytochemistry assays.

Supplier: Southern Biotechnology

Goat Anti-Human Ig Fc-AP antibody for use in ELISA assays.