2476 Results for: "STEMCELL Technologies"

Supplier: STEMCELL Technologies

Stimulating T cells with HHV6 (U90) Peptide Pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. HHV6 (U90) Peptide Pool is a lyophilized mixture of 267 peptides from protein U90 of human herpesvirus 6B (HHV-6B; strain Z29) and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 - 1078 on U90. U89 - U90 RNA expression occurs under immediate-early conditions of infection (Mirandola et al.), and the associated protein exhibits peri-nuclear and cytoplasmic localization (Nikolaou et al.). Also known as immediate-early protein IE1, latency-associated transcripts of HHV6 may function as a source of IE1 during viral reactivation (Kondo et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with the HPV18 (L1) Peptide Pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. A lyophilized mixture of 140 peptides from major capsid protein L1 of human papillomavirus type 18 (HPV18), HPV18 (L1) Peptide Pool consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 - 568 on L1. L1 enters the nuclei of host cells and can assemble into virus-like particles (Stauffer et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Green fluorescent lipophilic dye for staining cell membranes and other hydrophobic structures in live and fixed cells

Supplier: STEMCELL Technologies

Conveniently and consistently add transforming growth factor beta 1 (TGF-ꞵ1) to your cultures with this cell culture-ready formulation. This human recombinant TGF-ꞵ1 is precisely reconstituted to 0.1 mg/ml in 100 mM acetic acid and requires no additional preparation, improving reproducibility. Endotoxin levels are verified, using the LAL method to ensure consistency for use across multiple applications. A member of the TGF-β superfamily, TGF-β1 regulates diverse cellular phenotypes. TGF-β1 binds to serine-threonine kinase type I and II receptors and activates signal transduction through SMAD2/3 proteins.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG2b antibody against human CD68, APC-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD83, biotin-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD16, biotin-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2a, kappa isotype control antibody, FITC-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2b, kappa isotype control antibody, FITC-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human SSEA-5, PE-conjugated.

Supplier: STEMCELL Technologies

Interleukin 15 (IL-15) is a four-alpha helix bundle cytokine with many similar properties to IL-2. The IL-15 receptor is a heterotrimeric receptor composed of IL-15Ra (the high-affinity receptor for IL-15), as well as IL-2/15Rb (CD122) and common gamma chain (CD132). IL-15 binds to IL-15Rα receptor and can then be presented in trans to IL-2/15Rb and common gamma chain on other cells. Trans-presentation is thought to be the major mechanism by which IL-15-mediated responses occur in mice, although may not be necessary in humans (Castillo et al.). The cytoplasmic domains of IL-2/15Rb and common gamma chain mediate signaling to activate JAK/STAT and PI3K pathways. IL-15 supports the survival and proliferation of naïve CD4+ and CD8+ T cells, and promotes homeostasis of memory T cells. IL-15 also promotes the survival and differentiation of NK cells and regulates their cytolytic activity (Ma et al.). This product is animal component-free.

Supplier: STEMCELL Technologies

Rat monoclonal antibody against mouse CD3, APC-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal antibody against human IL-4, PE-conjugated.

Supplier: STEMCELL Technologies

Interleukin 1 alpha (IL-1α) is a member of the IL-1 family and a dual-function cytokine. Both the unprocessed precursor and a processed IL-1α protein signal through IL-1 receptor type 1 (IL-1R1). Various cells, including keratinocytes, thymic epithelium, hepatocytes, endothelial cells, fibroblasts, and the epithelial cells of mucous membranes, have high levels of intracellular IL-1α precursor. The precursor is also expressed on the surface of monocytes and B lymphocytes (Netea et al.). IL-1α recruits infiltrating cells to a site of injury during necrosis and plays an important role during processes of sterile inflammation (Cohen et al.; Rider et al.). During hypoxia, IL-1α contributes to angiogenesis (Carmi et al.). Studies in mice show that IL-1α is produced by microglia-like cells after ischemic brain injury, which contributes to the inflammation (Luheshi et al.).

Supplier: STEMCELL Technologies

Interleukin 1 alpha (IL-1α) is a member of the IL-1 family and a dual-function cytokine. Both the unprocessed precursor and a processed IL-1α protein signal through IL-1 receptor type 1 (IL-1R1). Various cells, including keratinocytes, thymic epithelium, hepatocytes, endothelial cells, fibroblasts, and the epithelial cells of mucous membranes have high levels of intracellular IL-1α precursor, which is also expressed on the surface of monocytes and B lymphocytes (Netea et al.). IL-1α recruits infiltrating cells to a site of injury during necrosis and plays an important role during processes of sterile inflammation (Rider et al.; Cohen et al.). During hypoxia, IL-1α contributes to angiogenesis (Carmi et al.). IL-1α is produced by microglia-like cells after ischemic brain injury, which contributes to the inflammation (Luheshi et al.).

Supplier: STEMCELL Technologies

Mouse monoclonal antibody against human CD69 (AIM).

Supplier: STEMCELL Technologies

Mouse monoclonal IgG2b, antibody against human, chimpanzee, CD45RA, Biotin-conjugated.

Supplier: STEMCELL Technologies

Hamster (Armenian) monoclonal antibody against mouse CD69, FITC-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2b, kappa isotype control antibody, unconjugated.

Supplier: STEMCELL Technologies

For detection and measurement of human interleukin 2.

Supplier: STEMCELL Technologies

For detection and measurement of compounds that inhibit binding of viral SARS-CoV-2 Spike protein to human ACE2 receptor.

Supplier: STEMCELL Technologies

Human Interleukin 4 (IL-4) is important for immune responses to helminth infection as well as in allergic responses (Olpihant et al.). The IL-4 receptor consists of a heterodimer of IL-4Ra and common gamma chain. IL-4 receptor engagement leads to the activation of JAK1/3 and the recruitment of STAT6 and IRS1/2 (Nelms et al.). IL-4 drives immunoglobulin class switching in B cells (to IgE, IgG4), mast cell hyperplasia, mucus production, and the differentiation of naïve T cells into Th2 cells, which produce IL-4, IL-5, IL-10, and IL-13 (Bao et al.; Nelms et al.; Olpihant et al.). In addition to Th2 cells, IL-4 is produced by CD4+ NK T cells, γ/δ T cells, activated basophils, eosinophils, and mast cells. Human IL-4 does not cross-react with mouse cells (Park et al.).

Supplier: STEMCELL Technologies

Assay buffer for labeling with Annexin V - 50 ml.

Supplier: STEMCELL Technologies

For detection and measurement of human interleukin 1 beta.

Supplier: STEMCELL Technologies

Defined, xeno-free induction medium for early mesodermal differentiation.

Supplier: STEMCELL Technologies

The EasyPlate™ EasySep™ Magnet is designed for cell separation procedures using selected EasySep™ reagents when processing a small number of cells (up to 2×10⁷ cells per well).

Supplier: STEMCELL Technologies

Stem cell factor (SCF) is an early-acting cytokine that plays a pivotal role in the regulation of embryonic and adult hematopoiesis. SCF promotes cell survival, proliferation, differentiation, adhesion, and functional activation of cells at multiple levels of the hematopoietic hierarchy. Together with other cytokines such as thrombopoietin and Flt3/Flk-2 Ligand, SCF is commonly used to promote expansion of primitive hematopoietic stem cells and multi-potent progenitor cells in culture (Martin et al.; Kent et al.). In synergy with various growth factors, including IL-2, IL-3, IL-6, IL-7, G-CSF, and erythropoietin, SCF increases proliferation and differentiation of myeloid and erythroid progenitor cells and a subset of lymphoid progenitor cells (Broudy). SCF is also a primary growth and activation factor for mast cells and eosinophils. SCF exists in two biologically active splice forms: a soluble and a transmembrane isoform. Upon binding to its receptor (c-Kit tyrosine kinase receptor; CD117), it activates PI3K, JAK/STAT, and MAPK pathways. SCF and signaling from c-Kit have also been reported to play an important role in pigmentation, fertility, vasculogenesis, motility of the gut via c-Kit positive interstitial cells of Cajal, and in the migration of neuronal stem and progenitor cells to sites of injury in the brain. This product is animal component-free.

Supplier: STEMCELL Technologies

Insulin-like growth factor 1 (IGF-I) is a polypeptide that belongs to the family of insulin-like growth factors that are similar in molecular structure to proinsulin. IGF-I binds to the IGF-I receptor and is a potent activator of the PI3K/AKT pathway and also activates ERK1/2 signaling. IGF-I is required for embryonic development, and it is produced mainly in the liver in response to a hepatocyte growth hormone. In the absence of insulin, IGF-I is necessary for the maintenance of human pluripotent stem cells (Wang et al.). Together with IL-3, IGF-I stimulates differentiation and proliferation of myeloid cells and has been shown to regulate lymphopoiesis by stimulating proliferation and differentiation of T and B cells in lymphoid organs (Heemskerk et al.).

Supplier: STEMCELL Technologies

Immunomagnetic isolation of human granulocytes directly from whole blood

Supplier: STEMCELL Technologies

50 ml centrifuge tube with an insert for density gradient centrifugation