29882 Results for: "Reagents"

Supplier: Rigaku Reagents

EBS-250-HEPES68 PH6.8 250ML

Supplier: REAGENTS DIRECT MS

INHIBITOR MEK 99.7% HPLC 2 MG

Supplier: REAGENTS DIRECT MS

CHIR99021 SELECTIVE INHIBITOR 2 MG

Supplier: RIGAKU REAGENTS MS

ZINC CHLORIDE 1M 100ML

Supplier: RIGAKU REAGENTS MS

MIB BUFFER 1M PH 9.0 100ML

Supplier: REAGENTS DIRECT MS

PD0325901 MEK INHIBITOR 99.7% HPLC 2MG

Supplier: RIGAKU REAGENTS MS

SODIUM PHOSPHATE CITRIC ACID 0.5M 100ML

Supplier: RIGAKU REAGENTS MS

AMMONIUM SULFATE 3.5M 100 ML

Supplier: Rigaku Reagents

SODIUM HYDROXIDE PH6.0 1M 100ML/VOLUME

Supplier: 3M Healthcare

CUNO™ CTG-Klean system filter pack with Betafine™ XL series filter cartridge is a totally enclosed filtration system, using a separate pressure vessel and disposable filter pack to isolate the product from the housing. Cartridge pack design reduces time and labor involved with change-outs and cleaning of the housing, helping to reduce worker exposure to cleaning solvents.

Supplier: RIGAKU REAGENTS MS

SALT SCREEN WIZARD PEG ION 48 PEG 8K/10K

Supplier: REAGENTS INC MS

SOLUTION SODIUM LAURYL SULFATE 0.004N 4L

Supplier: RIGAKU REAGENTS MS

SALT SCREEN WIZARD PEG ION 48 PEG 1K/4K

Supplier: RIGAKU REAGENTS MS

POLYETHYLENE GLYCL 5000 MME 50%W/V 250ML

Supplier: Siemens Healthineers

RAPIDPoint® 500 Blood Gas Analyzer from Siemens Healthcare Diagnostics delivers accurate, laboratory-quality test results for a comprehensive menu of critical care parameters in approximately 60 seconds, all from a single, whole-blood sample. The same RAPIDPoint® 500 Blood Gas Analyzer has been proven to address the need for accurate process analytics in all stages of development of bioprocessing applications.

Supplier: 3M Healthcare

CUNO™ CTG-Klean system filter pack with Betafine™ XL series filter cartridge is a totally enclosed filtration system, using a separate pressure vessel and disposable filter pack to isolate the product from the housing. Cartridge pack design reduces time and labor involved with change-outs and cleaning of the housing, helping to reduce worker exposure to cleaning solvents.

Supplier: MP Biomedicals

Protein kinase C inhibitor (IC50 = 50-200 µM depending on assay conditions in MCF-7 cells or IC50 = 100 µM in rat brain). The PKC inhibition is also dependent on the phosopholipid concentration. Also inhibits both calmodulin-dependent and calmodulin-independent Ca2+-, Mg2+-ATPase. Tamoxifen is a selective estrogen response modifier (SERM), anti-angiogenetic factor. It is a prodrug that is metabolized to active metabolites 4-hydroxytamoxifen (4-OHT) and endoxifen by cytochrome P450 isoforms CYP2D6 and CYP3A4. In breast cancer, the gene repressor activity of tamoxifen against ERBB2 is dependent upon PAX2. Blocks estradiol-stimulated VEGF production in breast tumor cells.
Tamoxifen has been used to facilitate the recombination of ect2flox allele in mouse organs. It has also been used to study its effect on lipopolysaccharide (LPS)-induced microglial activation
Tamoxifen is a Protein kinase C inhibitor. It induces apoptosis in human malignant glioma cell lines. Tamoxifen and its metabolite 4-hydroxytamoxifen are selective estrogen response modifiers (SERMs) that act as estrogen antagonists in mammary gland. Blocks estradiol-stimulated VEGF production in breast tumor cells.

Supplier: 3M Healthcare

Filter cartridges made of two asymmetric polyethersulfone (PES) membrane layers with Advanced Pleat Technology in manufacturing LifeASSURE™ PDA Series Filter Cartridges, creating a filter that provides high throughput and fast flow. These filters have applications in pre-filtration and sterilizing filtration in the pharmaceutical, biological, and bioprocess industries where low protein binding is critical. PP end cap adapters, inner core and outer cage, membrane support layer. Polysulfone encapsulated adapter reinforcing ring (5 to 40" cartridge configurations only).

Supplier: Harvard Apparatus

The Gemini Twin Wave Electroporators are flexible systems allowing both square wave and exponential decay wave electroporation in a single unit. These waveform combinations enable researchers to easily and efficiently electroporate eukaryotic and prokaryotic cells in cuvettes or plates with one easy-to-use setup. Prokaryotic cells typically respond well to exponential decay wave pulses and eukaryotic cells are transfected most efficiently with square wave pulses. Combining these two waveforms gives researchers total flexibility to achieve the highest efficiency for their applications.

Supplier: ReVacc Scientific

This kit is developed to measure the levels of human Kallikrein 3/Prostate Specific Antigen (KLK3/PSA) concentrations in cell culture supernates, serum, plasma or biological fluids.

Supplier: Rockland Immunochemical

Anti-Cyclin D3 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Cyclin D3 belongs to a highly conserved cyclin family, whose members are the ultimate recipients of oncogenic signals. Cyclin D3 is a key component of the cell cycle progression machinery and induces progression through the G1 phase of the cell cycle. Cyclin D3 is expressed in nearly all proliferating cells, and shows the most broad expression pattern of all three D-type (D1-D3) cyclins. Cyclin D3 is encoded from the 6p21 chromosome region and the protein is predominantly localized in the nucleus. Once induced, cyclin D3 binds and activates its associated cyclin-dependent kinases CDK4 and CDK6. Amplification of the cyclin D3 gene and overexpression of cyclin D3 protein is seen in several human cancers. A large number of human malignancies contain lesions in pathways impacting on cyclin D3. Abnormal expression of Cyclin D3 is believed to be a driving force in several human cancers. A possible role for cyclin D3 in the malignancies of the lymphoid system is suggested by the observations that cyclin D3 gene is rearranged in several neoplastic diseases such as diffuse large B cell lymphomas or multiple myelomas. Anti-Cyclin D3 is ideal for researchers interested in Cancer Research and Immunology research.

Supplier: MP Biomedicals

Folic acid, also known as folate, is a B vitamin that can be found in a variety of fruits and vegetables. It can also be chemically synthesized. Folate, a watersoluble vitamin, helps the body form red blood cells and aids in the formation of genetic material within every body cell. This product exhibits metal binding properties. Hematopoietic vitamin present, free or combined with one or more additional molecules of L-(+)-glutamic acid, in liver, kidney, mushrooms, spinach, yeast, green leaves, and grasses.
Folic acid (Vitamin B9 and folate) is essential to numerous bodily functions. The human body needs folate to synthesize DNA, repair DNA, and methylate DNA as well as to act as a cofactor in certain biological reactions. It is especially important in aiding rapid cell division and growth, such as in infancy and pregnancy.
Folic acid (FA) and dihydrofolic acid (FAH2) are substrates of dihydrofolate reductase(s) which reduce them to tetrahydrofolate (THF), which in turn supports ‘one carbon’ transfer. Tetrahydrofolates are required for de novo synthesis of purines, thymidylic acid and various amino acids and for post-translational methylation (epigenetics).
Very slightly soluble in cold water (0.0016 mg/mL at 25 °C), soluble to about 1% in boiling water. Slightly soluble in methanol, appreciably less soluble in ethanol and butanol. Insoluble in acetone, chloroform, ether, benzene. Relatively soluble in acetic acid, phenol, pyridine, solutions of alkali hydroxides and carbonates. Soluble in hot dilute HCl and H2SO4.

Supplier: Thermo Scientific Chemicals

Powder

Supplier: GILSON, INC.

This compact, ergonomic system is an easy to use and accurate solution for high throughput pipetting of 96- and 384-well plates. The PLATEMASTER's 96-channel design greatly reduces the number of pipetting steps necessary to fill a microplate when compared to using regular manual multichannel pipettes. When using this, the time it takes to fill 96-well plates is significantly reduced to approximately 10 to 20 seconds or less, and 384-wells can typically be filled in less than a minute using only four pipetting steps.

Supplier: TCI America

CAS Number: 464-49-3 MDL Number: MFCD00064149 Molecular Formula: C10H16O Molecular Weight: 152.24 Purity/Analysis Method: 98.0% (GC) Form: Crystal Boiling point (°C): 204 Melting point (°C): 178 Flash Point (°C): 66 Specific rotation [a]20/D: 44.5 deg (C=20, EtOH)

Supplier: MP Biomedicals

β-NADP is a coenzyme necessary for the alcoholic fermentation of glucose and the oxidative dehydrogenation of other substances. It occurs widely in living tissue, especially in the liver. Nicotinic acid can be converted to nicotinamide in the body and, in this form, is found as a component of two oxidation-reduction coenzymes: nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP). The nicotinamide portion of the coenzyme transfers hydrogens by alternating between oxidized quaternary nitrogen and a reduced tertiary nitrogen. NADP is an essential coenzyme for glucose-6-phosphate dehydrogenase which catalyzes the oxidation of glucose-6-phosphate to 6-phosphogluconic acid. This reaction initiates metabolism of glucose by a pathway other than the citric acid cycle. This route is known as the hexose phosphate shunt or phosphogluconate pathway. Other enzymes which utilize NADP as a coenzyme are: Alcohol dehydrogenase:NADP dependent; Aromatic ADH:NADP dependent; Ferredoxin-NADP reductase; L-Fucose dehydrogenase; Gabase; Galactose-1-phosphate uridyl transferase; Glucose dehydrogenase; L-Glutamic dehydrogenase; Glycerol dehydrogenase:NADP specific; Isocitric dehydrogenase; Malic enzymes; 5,10-Methylenetetrahydrofolate dehydrogenase; 6-Phosphogluconate dehydrogenase and Succinic semialdehyde dehydrogenase.

Supplier: Genetex

Jun activation domain-binding protein 1 (JAB1) also designated COP9 subunit 5 (COPS5) or SGN5 is a coactivator of AP1 transcription factor that also promotes degradation of the cyclin-dependent kinase inhibitor p27Kip1. JAB1 interacts with c-Jun AP1 containing complexes, and enhances transactivation from AP1 dependent promoters. It also interacts with Jun D but not with Jun B or v-Jun. JAB1 is highly conserved in evolution and is widely expressed in mammalian tissues. It is localized both the nucleus and the cytoplasm. It interacts with the cytoplasmic domain of the alphaL/beta2 integrin LFA1. Following LFA1 engagement the nuclear pool of JAB1 increases and activation of an AP1 driven promoter is enhanced. Interaction of JAB1 with the nuclear progesterone receptor and the steroid receptor activator (SRC1) was reported. JAB1 is a stability and activity regulator of Hypoxia - inducible factor 1 (HIF1), a transcription factor that controls activation of several genes responsive to the cellular oxygen tension. The macrophage migration inhibitory factor (MIF) associates with JAB1 in the cytosol near the plasma membrane. Endogenous MIF inhibits JAB1-induced AP1 transcriptional activity. JAB1 is a subunit of the COP9 regulatory complex. COP9 cleaves the ubiquitin-like protein Nedd8 from the Cul1 subunit of SCF ubiquitin ligases. A metalloprotease motif in JAB1 plays a role in this isopeptidase activity. Breakdown of the cyclin dependent kinase inhibitor p27Kip1 is promoted by JAB1. The latter expression in several cancers inversely correlates with p27Kip1 and may reflect tumor aggressiveness. A possible involvement of JAB1 in atherosclerosis was also reported. Involvement of JAB1 in degradation of the suppressors p53 and smad4 was described recently.

Supplier: MP Biomedicals

Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose; used as a diuretic; enhances the action of sulfonamides; an antiseptic.
Urea in solution is in equilibrium with ammonium cyanate. The form that reacts with protein amino groups is isocyanic acid. Urea in the presence of heat and protein leads to carbamylation of the proteins. Carbamylation by isocyanic acid interferes with protein characterization because isocyanic acid reacts with the amino terminus of proteins, preventing N-terminal sequencing. Isocyanic acid also reacts with side chains of lysine and arginine residues resulting in a protein that is unsuitable for many enzymatic digests. In addition, carbamylation often leads to confusing results from peptides having unexpected retention times and masses. When performing enzymatic protein digests it is important to remove urea first. Even though some enzymes will tolerate small amounts of urea, the elevated temperature used for most reactions will lead to carbamylation during the course of the digest. The urea can be removed prior to digestion by fast reversed phase chromatography, spin columns, or dialysis.
Dissolve urea in deionized water to the desired concentration.For every 10 ml of solution, add 1 g of Amberlite® IRA-910.Stir for one hour at room temperature

Supplier: StatLab

The Supa Mega Slim cassette is being widely adopted in clinical, research, and toxicology laboratories.

Supplier: StatLab

With an internal cavity a third of the depth of the conventional Supa Mega, the PathFlow Supa Mega Mothership Cassette provides an innovative solution for the processing, embedding, and tracking of large tissue specimens.