108 Results for: "QUALITY BIOLOGICAL, INC."

Supplier: QUALITY BIOLOGICAL, INC.

Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow.

Supplier: QUALITY BIOLOGICAL, INC.

DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.

Supplier: QUALITY BIOLOGICAL, INC.

Most media are buffered by a combination of sodium bicarbonate and an atmosphere enriched in CO2. The latter is usually provided through the use of a specially equipped incubator. L-15 was developed at a time when such equipment was not readily available. Dr. Leibovitz developed a buffering system based on free base amino acids, inorganic salts and the substitution of galactose for glucose.

Supplier: QUALITY BIOLOGICAL, INC.

It is important to store cells for future studies.

Supplier: QUALITY BIOLOGICAL, INC.

TE is composed of buffering agent Tris, and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation.

Supplier: QUALITY BIOLOGICAL, INC.

Dr. Williams and his coworkers’ studies on hepatocytes in culture led to the development of Williams’ Medium E.

Supplier: QUALITY BIOLOGICAL, INC.

DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. We offer a variety of DMEM modifications for a range of cell culture applications.

Supplier: QUALITY BIOLOGICAL, INC.

PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications.

Supplier: QUALITY BIOLOGICAL, INC.

L-glutamine is a cell culture supplement, and an essential amnio acid. L-glutamine is required in cell culture for cellular growth.

Supplier: QUALITY BIOLOGICAL, INC.

LB Agar, Miller and LB Broth, Miller are based on LB Medium as described by Miller for the growth and maintenance of E. coli strains used in molecular microbiology procedures. These are nutritionally rich media designed for growth of pure cultures of recombinant strains.

Supplier: QUALITY BIOLOGICAL, INC.

QBI’s Water, Cell Culture, WFI Quality is high quality water for use as a solvent in the preparation of cell culture media and biochemical reagents.

This water is extensively purified by reverse osmosis, subsequently deionized and passed through a filter membrane to remove particles and tested to meet the specifications found in the USP (United States Pharmacopeia) monograph for Water for Injection (WFI) for conductivity, endotoxin,and TOC. It is additionally tested for sterility, mycoplasma, and cell culture performance.

This water is tested against USP specifications, but is not intended for injection. It is only intended for research or further manufacturing use, not for diagnostic or therapeutic use.

Supplier: QUALITY BIOLOGICAL, INC.

Hanks' Balanced Salt Solution (HBSS) provides a buffering system to maintain the physiological pH range and osmotic balance of the culture media. HBSS provides cells with a source of water and essential inorganic ions, and carbohydrate as an energy source.

Supplier: QUALITY BIOLOGICAL, INC.

Sodium acetate is a widely used reagent in molecular biology applications. It is used as a buffer in conjunction with acetic acid, in the buffering range of pH 3.6 – 5.6.

Supplier: QUALITY BIOLOGICAL, INC.

Terrific Broth was developed by Tartoff and Hobbs to increase yields in plasmid bearing E. coli. Terrific Broth is an enriched medium, which helps the recombinant strains of E. coli maintain an extended growth phase. The large amount of tryptone and yeast extract in the medium allows the bacteria to produce a higher plasmid yield.

Supplier: QUALITY BIOLOGICAL, INC.

DNA Gel Loading Solutio, 5X is prepared according to Sambrook, Fritsch & Maniatis. The purpose of this reagent is to be a tracking dye during electrophoresis. It ensures that DNA samples remain submerged in the wells of the gel, by making them denser than the electrophoresis buffer.

Supplier: QUALITY BIOLOGICAL, INC.

EDTA (Ethylenediaminetetraacetic acid), a metal chelator, is widely used in molecular biology as a chemical reagent to reduce nuclease activity (i.e. metalloenzyme). EDTA solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and reagents, inorganic salts, and chelators.

Supplier: QUALITY BIOLOGICAL, INC.

Magnesium is a typical ionic halide, and is very soluble in water. Magnesium has a variety of biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid structure. Magnesium is an essential co-factor in many enzymes, including DNAse, some restriction enzymes, and Ribonuclease H.

Supplier: QUALITY BIOLOGICAL, INC.

RNA Gel Loading Solution, 10X is prepared according to Sambrook, Fritsch & Maniatis (1989)

Supplier: QUALITY BIOLOGICAL, INC.

Super Broth is used for the mass cultivation of Escherichia coli for high yield protein and plasmid DNA production.

Supplier: QUALITY BIOLOGICAL, INC.

Kanamycin sulfate is a water soluble broad spectrum antibiotic obtained from soil bacterium Streptomyces kanamyceticus. It is an aminoglycoside antibiotic effective against Gram-negative and Gram-positive bacteria and mycoplasma. It binds to the 30S ribosomal subunit, inhibits translocation, and elicits miscoding.

Supplier: QUALITY BIOLOGICAL, INC.

USDA FBS is used as a supplement in classical cell culture media. USDA FBS both nourishes and protects the cells in a way that almost nothing else has been found to do.

Supplier: QUALITY BIOLOGICAL, INC.

Non Fat Dry Milk

Supplier: QUALITY BIOLOGICAL, INC.

Cryopreservation Medium is a ready-to-use medium for the preservation of cells as seed stock or for future investigative study. Cryopreservation allows you to preserve and protect your cell line stock(s) from over culturing that can lead to unwanted changes within cellular properties or lead to senescence.

Supplier: QUALITY BIOLOGICAL, INC.

YPG Agar is recommended for the growth of Saccharomyces cerevisiae for molecular biology purpose.

Supplier: QUALITY BIOLOGICAL, INC.

Compared to other basal media, Ham’s F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Ham’s F-12 contains no proteins or growth factors, and uses a sodium bicarbonate buffer system (1.176 g/L); therefore requiring a 5–10% CO2 environment to maintain physiological pH.

Supplier: QUALITY BIOLOGICAL, INC.

TBE (TRIS Borate EDTA) is often used in procedures involving nucleic acids, the most common being electrophoresis in molecular biology. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water.

Supplier: QUALITY BIOLOGICAL, INC.

Suitable for molecular biology, protein chemistry, and biochemical applications.

Supplier: QUALITY BIOLOGICAL, INC.

Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. These solutions of Tris hydrochloride provide convenient and highly purified solutions.

Supplier: QUALITY BIOLOGICAL, INC.

MEM NEAA contains the same non-essential amino acids found in the standard MEM, at a strength of 100. MEM NEAA 100X can be used to enhance cell growth and prolong the viability of cells in culture. MEM NEAA 100X is commonly used as a supplement to minimal basal medium, and does not dilute the components already in the cell culture medium.

Supplier: QUALITY BIOLOGICAL, INC.

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). Although, RPMI-1640 has been shown to support the serum-free growth of a number of cells.