1380 Results for: "New England Biolabs (NEB)"

Supplier: New England Biolabs (NEB)

An affinity matrix for the small-scale isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins in manual or automated formats. Immobilized Metal Affinity Chromatography (IMAC) purifications employing Ni-NTA (nickel-nitrilotriacetic acid) magnetic beads can be performed under native or denaturing conditions which permit efficient binding and purification of insoluble proteins, proteins that aggregate in inclusion bodies, or proteins that possess a tertiary structure that sequester the polyhistidine affinity tag.

Supplier: New England Biolabs (NEB)

The Monarch® gDNA Tissue Lysis Buffer is a component of the Monarch® Genomic DNA Purification Kit (NEB #T3010), which can be used to purify up to 30 µg genomic DNA from a variety of sample types.

Supplier: New England Biolabs (NEB)

The NEBNext ARTIC kits were developed in response to the critical need for reliable and accurate methods for sequencing SARS-CoV-2.

Supplier: New England Biolabs (NEB)

The Monarch® pestle set contains single-use microtube pestles and the accompanying 1.5 ml tubes for sample disruption and homogenization prior to nucleic acid extraction. 100 green pestles and tubes are included in this set.

Supplier: New England Biolabs (NEB)

The Monarch® Protein separation solution, supplied as a component of the Monarch HMW DNA extraction kit for tissue, is a salt-based solution that is optimized to ensure proteins in tissue and bacteria samples are easily removed during the phase separation of the Monarch HMW DNA extraction workflow.

Supplier: New England Biolabs (NEB)

NEBExpress® E. coli Lysis Reagent is a chemical lysis solution composed of a proprietary mix of non-ionic and zwitterionic detergents and Tris-based buffer. It allows disruption of E. coli cells without denaturing soluble proteins.

Supplier: New England Biolabs (NEB)

The Luna Probe One-Step RT-qPCR Mix with UDG (No ROX) is supplied at a 4X concentration and enables higher amounts of sample input, which is relevant for applications where RNA present in low abundance is of interest, such as pathogen detection.

Supplier: New England Biolabs (NEB)

The NEBNext Ultra II DNA PCR-free Library Prep Kit contains the enzymes and buffers required to convert a broad range of intact DNA input amounts into libraries for next-generation sequencing on the Illumina platform, all without an amplification step.

Supplier: New England Biolabs (NEB)

Boletopsis grisea Lectin (BGL) is a recombinant 15 kDa lectin from the Boletopsis grisea mushroom that has been expressed in E. coli.

Supplier: New England Biolabs (NEB)

Faustovirus Capping Enzyme (FCE) catalyzes the addition of N7-methylguanosine cap (m7G) to the 5' end of triphosphorylated and diphosphorylated transcripts, producing Cap-0 RNA. FCE is a single-subunit enzyme that combines the three activities necessary to produce the Cap-0 structure- triphosphatase, guanylyltransferase, and (guanine-N7)-methyltransferase. Installation of Cap-0 is a key step in eukaryotic mRNA maturation along with 2'-O-methylation at position 1 (Cap-1) and polyadenosine (poly(A)) tailing.

Supplier: New England Biolabs (NEB)

A DNA specific exonuclease isolated from Methylocaldum szegediense that catalyzes the removal of nucleotides from linear single-stranded DNA in the 3' to 5' direction with optimal activity between 45 and 60 °C. Isolated from a strain of E. coli which carries the cloned pMC45-1 gene from Methylocaldum szegediense.

Supplier: New England Biolabs (NEB)

Induro reverse transcriptase is a group II intron-encoded RT that exhibits increased thermostability, high processivity, and increased tolerance of inhibitors in the synthesis of cDNA from RNA.

Supplier: New England Biolabs (NEB)

The SARS-CoV-2 Positive Control (N gene) is a plasmid that contains the SARS-CoV-2 N-gene (GenBank: MN908947.3). It includes a T7 RNA promoter sequence for generation of RNA transcripts, if necessary. This product is used for assay validation of RT-qPCR, LAMP, or other detection methods for SARS-CoV-2 that target the N gene. The concentration is approximately 1000000 copies/µl.

Supplier: New England Biolabs (NEB)

New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers.

Supplier: New England Biolabs (NEB)

The NEB Golden Gate Assembly Kit (BsmBI-v2) contains an optimized mix of BsmBI-v2 and T4 DNA Ligase. BsmBI-v2 has been engineered by NEB and outperforms BsmBI in Golden Gate Assemblies.

Supplier: New England Biolabs (NEB)

This contains 0.25 ml of 100 mM ultrapure dUTP.

Supplier: New England Biolabs (NEB)

For optimal workflow flexibility, NEBNext® Multiplex Oligos are available separately from library preparation kits, providing adaptors and primers for high-yield multiplex Illumina® library production. 

Supplier: New England Biolabs (NEB)

The Luna SARS-CoV-2 RT-qPCR Multiplex assay kit is optimized for real-time qualitative detection of SARS-CoV-2 nucleic acid using hydrolysis probes.

Supplier: New England Biolabs (NEB)

The LAMP fluorescent dye is an intercalating dye that can be used to track a LAMP reaction in real-time by fluorescence measurement.

Supplier: New England Biolabs (NEB)

Methyltransferase deficient chemically competent E.coli cells suitable for growth of plasmids free of Dam and Dcm methylation.

Supplier: New England Biolabs (NEB)

An E.coli strain that carries the BtsCI gene from Bacillus thermosphaericus (X. Pan).

Supplier: New England Biolabs (NEB)

An E.coli strain that carries the AvaI gene from Anabaena variabilis (ATCC 27892).

Supplier: New England Biolabs (NEB)

E. coli RecA is necessary for genetic recombination, reactions involving DNA repair and UV-induced mutagenesis. RecA promotes the autodigestion of the lexA repressor, umuD protein and lambda repressor. Cleavage of LexA derepresses more than 20 genes. In vitro studies indicate that in the presence of ATP, RecA promotes the strand exchange of single-strand DNA fragments with homologous duplex DNA. The reaction has three distinct steps: (i) RecA polymerizes on the single-strand DNA, (ii) the nucleoprotein filament binds the duplex DNA and searches for a homologous region, (iii) the strands are exchanged.

Supplier: New England Biolabs (NEB)

Klenow Fragment (3´→ 5´ exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5´→ 3´ exonuclease activity and has mutations (D355A, E357A) which abolish the 3´→ 5´ exonuclease activity.

Supplier: New England Biolabs (NEB)

Catalyzes the stepwise removal of mononucleotides from 3´-hydroxyl termini of duplex DNA.

Supplier: New England Biolabs (NEB)

Deoxynucleotide (dNTP) Solution Mix is an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP.

Supplier: New England Biolabs (NEB)

Chemically competent Kluyveromyces lactis cells suitable for transformation with a linearized pKLAC-series expression vector

Supplier: New England Biolabs (NEB)

Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7)

Supplier: New England Biolabs (NEB)

Micrococcal nuclease is derived from Staphylococcus aureus and is a relatively non-specific endo-exonuclease

Supplier: New England Biolabs (NEB)

The PreCR Repair Mix is an enzyme cocktail formulated to repair damaged template DNA prior to its use in the polymerase chain reaction (PCR), microarrays, or other DNA technologies.