911 Results for: "Molecular Biology Reagents"

Supplier: MP Biomedicals

Adenosine is a purine nucleoside comprising a molecule of adenine attached to a ribose sugar molecule moiety via a β-N9-glycosidic bond. It plays an important role in energy transfer. It is an endogenous purine nucleoside that modulates physiological process wherein cellular signaling by adenosine occurs through four known adenosine receptor subtypes (A1, A2A, A2B, and A3) Adenosine is an anti-inflammatory agent at the A(2A) receptor. Cardioprotective effects may relate to activation of A1 adenosine receptors. Endogenous neurotransmitter at adenosine receptors. Cardioprotective effects may relate to activation of A1 adenosine receptors. The antiplatelet and anti−inflammatory actions of adenosine appear to be mediated via the A2 adenosine receptor. In contrast, adenosine appears to be a pro-inflammatory mediator in asthma and chronic obstructive pulmonary disease (COPD). Adenosine is used in topical treatment of foot wounds in diabetes mellitus and also used for the treatment or prevention of cardiac arrhythmias (irregular heartbeat).It is used to cause dilation of the blood vessels. Physical Appearance: White powder Optical Rotation: -68.0° to -72.0° (c=2, NaOH 1:20) Heavy Metals: ≤0.001% Loss on Drying: ≤0.5% Residue on Ignition: ≤0.1% Storage Temp. : -20 °C

Supplier: MP Biomedicals

Tunicamycin is a mixture of Tunicamycins A, B, C and D. It is a nucleoside antibiotic active in vitro against Gram-positive bacteria, yeasts, fungi, and viruses.

Supplier: VIAGEN BIOTECH INC MS

DirectPCR® DNA extraction system is a single-tube system for rapid preparation of DNA from mouse tails, ear pieces, yolk sac, and culture cells.

Supplier: Agilent Technologies

The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. In addition, the XL version of the kit is specially designed for efficient mutagenesis of large (4 -14 kb) or otherwise difficult-to mutagenize plasmid templates. The XL kit features components specifically designed for more efficient DNA replication and bacterial transformation.

Supplier: MP Biomedicals

Physiological chelating agent for heavy metals.
It is used as an antirheumatic and as a chelating agent in Wilson′s disease. It is used as a copper chelator to form mixed disulfides with cysteine or other sulfide media components. It is used to inactivate protein-1 DNA binding and to inhibit the growth of asynchronous cultures of rabbit articular chondrocytes.
Penicillamine is a characteristic degradation product of penicillin type antibiotics. One atom of copper combines with two molecules of penicillamine. Penicillamine reduces excess cystine excretion in cystinuria. This is by disulfide interchange between penicillamine and cystine, which results in formation of a readily excreted penicillamine-cysteine disulfide. Penicillamine interferes with the formation of cross-links between tropocollagen molecules and cleaves them when newly formed. Penicillamine lowers IgM rheumatoid factor and depresses T-cell activity.
+4°C

Supplier: Invitrogen

Thermo Scientific Pierce SDAD (NHS-SS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 13.5Å spacer arm containing a cleavable disulfide bond separates the two photoreactive groups.

Supplier: MP Biomedicals

Deoxycholic Acid Sodium Salt Monohydrate is an anionic biological detergent for the solubilization of membrane-bound proteins.

Supplier: MP Biomedicals

Bilirubin is the principal pigment of bile and constituent of many biliary calculi and also found in blood. As the major end-product of the biological breakdown of heme, bilirubin is the chromophore responsible for coloration in various forms of jaundice.
Bilirubin is suitable for use in the preparation of standard stock solutions of bilirubin for color density comparison in the determination of serum bilirubin.
It appears to function as an antioxidant and efficient peroxyl radical scavenger, protecting membrane lipids from oxidation by these radicals. At nanomolar concentrations it has been shown to protect neurons from oxidative damage.
Bilirubin is produced from Ox-gall which is sterilized before extraction with high pressure vapour at 120 °C. Then the bilirubin is extracted in a continuous extraction process with chloroform as a crude product. Recrystallization and purification is with ethanol and chloroform.
-20°C. Protect from light.

Supplier: MP Biomedicals

Cholic acid is a biochemical solubilizing agent (a non-ionic, non-denaturing detergent). Occurs inconjugation with glycine or taurine in bile of most vertebrates.

Supplier: Invitrogen

Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

Supplier: Invitrogen

Thermo Scientific Pierce Sulfo-LC-SDA (Sulfo-NHS-LC-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 12.5Å spacer arm separates the two photoreactive groups.

Supplier: Invitrogen

Thermo Scientific Pierce LC-SDA (NHS-LC-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 12.5Å spacer arm separates the two photoreactive groups.

Supplier: MP Biomedicals

The FastPROTEIN blue kit is designed for rapid isolation and purification of protein from bacterial cultures.

Supplier: Invitrogen

Thermo Scientific Pierce TCEP-HCl is a potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. The unique compound is easily soluble and very stable in many aqueous solutions. TCEP reduces disulfide bonds as effectively as dithiothreitol (DTT), but unlike DTT and other thiol-containing reducing agents, TCEP does not have to be removed before certain sulfhydryl-reactive crosslinking reactions.

Supplier: Invitrogen

Thermo Scientific Pierce Sulfo-SDA (Sulfo-NHS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 3.9Å spacer arm separates the two photoreactive groups.

Supplier: MP Biomedicals

Ammonium sulfate is an inorganic salt prepared by treating ammonia with sulfuric acid. Ammonium sulfate decomposes upon heating above 250 °C, forming ammonium bisulfate.

Supplier: Invitrogen

Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

Supplier: BENTO BIOWORKS LTD SE

The mobile lab that includes a thermal cycler, powered electrophoresis, blue light transilluminator, and centrifuge, all into one unit.

Supplier: MP Biomedicals

Paromomycin Sulfate is an aminoglycoside antibiotic which inhibits protein initiation.

Supplier: STEMCELL Technologies

DAPI (4',6-diamidino-2-phenylindole dihydrochloride) is a blue-fluorescent dye that binds to AT-rich regions of double-stranded DNA.

Supplier: BIOASSAY SYSTEMS

For quantitative determination of DNA.

Supplier: Invitrogen

Thermo Scientific Pierce LC-SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long cyclohexane-stabilized spacer arm (16.2 angstroms).

Supplier: Invitrogen

Thermo Scientific Pierce Sulfo-SDAD (Sulfo-NHS-SS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 13.5Å spacer arm containing a cleavable disulfide bond separates the two photoreactive groups.

Supplier: Invitrogen

Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.

Supplier: Promega Corporation

The PolyATtract System isolates messenger RNA directly from total RNA.

Supplier: Invitrogen

Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.

Supplier: Promega Corporation

The PolyATtract System isolates messenger RNA directly from total RNA.

Supplier: Promega Corporation

The PolyATtract System isolates messenger RNA directly from total RNA.

Supplier: MP Biomedicals

Arachidonic Acid is an essential fatty acid. Occurs in liver, brain, glandular organs, and depot fats of animals, in small amounts in human depot fats, and is a constituent of animal phosphatides.
Arachidonic Acid is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes. Arachidonic acid plays a key role in cellular regulation and is controlled through multiple interconnected pathways.
Arachidonic acid (AA) is an unsaturated ω6 fatty acid constituent of the phospholipids of cell membranes. Phospholipase A2 releases AA from the membrane phospholipids in response to inflammation. AA is subsequently metabolized to prostaglandins and thromboxanes by at least two cyclooxygenase (COX) isoforms, to leukotrienes and lipoxins by lipoxygenases, and to epoxyeicosatrienoic acids via cytochrome p450-catalyzed metabolism. AA and its metabolites play important roles in a variety of biological processes, including signal transduction, smooth muscle contraction, chemotaxis, cell proliferation and differentiation, and apoptosis. AA has been demonstrated to bind to the a subunit of G protein and inhibit the activity of Ras GTPase-activating proteins (GAPs). Cellular uptake of AA is energy dependent and involves protein-facilitated transport across the plasma membrane.
If ethanol is undesirable, arachidonic acid may be dissolved in acetonitrile, DMF, or DMSO. Simply evaporate the ethanol under a gentle stream of nitrogen (be certain not to evaporate the material to dryness) and redissolve the arachidonic acid in the solvent of choice.Just prior to use, make dilutions of the stock solution into aqueous buffer or isotonic saline to bring the arachidonic acid to the desired concentration. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiologic effects at low concentrations. A control using the solvent in the absence of the prostaglandin will address this potential variable. We do not recommend storing the aqueous solution for more than one day. It is difficult to obtain aqueous solutions of arachidonic acid directly. However, an organic solvent free solution of arachidonic acid can be prepared using concentrated basic buffers (pH > 8.0 and ionic strength not less than 0.1 M). Add 400 μL of cold buffer (0 °C) per mg of arachidonic acid and agitate vigorously and/or ultrasonicate.

Supplier: Invitrogen

Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.