914 Results for: "Molecular Biology Reagents"

Supplier: Invitrogen

Thermo Scientific Pierce DSS, also called disuccinimidyl suberate, is noncleavable and membrane permeable crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of an 8-carbon spacer arm.

Supplier: Invitrogen

Thermo Scientific Pierce SMPT is a long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form strong (hindered) yet cleavable (reducible) disulfide bonds with cysteine sulfhydryls..

Supplier: MP Biomedicals

Bilirubin is the principal pigment of bile and constituent of many biliary calculi and also found in blood. As the major end-product of the biological breakdown of heme, bilirubin is the chromophore responsible for coloration in various forms of jaundice.
Bilirubin is suitable for use in the preparation of standard stock solutions of bilirubin for color density comparison in the determination of serum bilirubin.
It appears to function as an antioxidant and efficient peroxyl radical scavenger, protecting membrane lipids from oxidation by these radicals. At nanomolar concentrations it has been shown to protect neurons from oxidative damage.
Bilirubin is produced from Ox-gall which is sterilized before extraction with high pressure vapour at 120 °C. Then the bilirubin is extracted in a continuous extraction process with chloroform as a crude product. Recrystallization and purification is with ethanol and chloroform.
-20°C. Protect from light.

Supplier: MP Biomedicals

Cholic acid is a biochemical solubilizing agent (a non-ionic, non-denaturing detergent). Occurs inconjugation with glycine or taurine in bile of most vertebrates.

Supplier: MP Biomedicals

Adenosine is a purine nucleoside comprising a molecule of adenine attached to a ribose sugar molecule moiety via a β-N9-glycosidic bond. It plays an important role in energy transfer. It is an endogenous purine nucleoside that modulates physiological process wherein cellular signaling by adenosine occurs through four known adenosine receptor subtypes (A1, A2A, A2B, and A3) Adenosine is an anti-inflammatory agent at the A(2A) receptor. Cardioprotective effects may relate to activation of A1 adenosine receptors. Endogenous neurotransmitter at adenosine receptors. Cardioprotective effects may relate to activation of A1 adenosine receptors. The antiplatelet and anti−inflammatory actions of adenosine appear to be mediated via the A2 adenosine receptor. In contrast, adenosine appears to be a pro-inflammatory mediator in asthma and chronic obstructive pulmonary disease (COPD). Adenosine is used in topical treatment of foot wounds in diabetes mellitus and also used for the treatment or prevention of cardiac arrhythmias (irregular heartbeat).It is used to cause dilation of the blood vessels. Physical Appearance: White powder Optical Rotation: -68.0° to -72.0° (c=2, NaOH 1:20) Heavy Metals: ≤0.001% Loss on Drying: ≤0.5% Residue on Ignition: ≤0.1% Storage Temp. : -20 °C

Supplier: MP Biomedicals

GeneClean® Turbo kit is used for purification of DNA fragments of sizes 0.1 to 300 kb from agarose gels, PCR reactions and other enzymatic solutions. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.

Supplier: VIAGEN BIOTECH INC MS

DirectPCR® DNA extraction system is a single-tube system for rapid preparation of DNA from mouse tails, ear pieces, yolk sac, and culture cells.

Supplier: Agilent Technologies

The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. In addition, the XL version of the kit is specially designed for efficient mutagenesis of large (4 -14 kb) or otherwise difficult-to mutagenize plasmid templates. The XL kit features components specifically designed for more efficient DNA replication and bacterial transformation.

Supplier: Invitrogen

Thermo Scientific Pierce DMP is dimethyl pimelimidate, a membrane-permeable crosslinker that contains an amine-reactive imidoester group at each end of a 7-atom spacer arm.

Supplier: Cytiva

Sera-Xtracta™ HMW DNA Kit offers magnetic bead-based extraction and purification of genomic DNA from whole blood.

Supplier: MP Biomedicals

Tunicamycin is a mixture of Tunicamycins A, B, C and D. It is a nucleoside antibiotic active in vitro against Gram-positive bacteria, yeasts, fungi, and viruses.

Supplier: MP Biomedicals

The FastPROTEIN blue kit is designed for rapid isolation and purification of protein from bacterial cultures.

Supplier: MP Biomedicals

Physiological chelating agent for heavy metals.
It is used as an antirheumatic and as a chelating agent in Wilson′s disease. It is used as a copper chelator to form mixed disulfides with cysteine or other sulfide media components. It is used to inactivate protein-1 DNA binding and to inhibit the growth of asynchronous cultures of rabbit articular chondrocytes.
Penicillamine is a characteristic degradation product of penicillin type antibiotics. One atom of copper combines with two molecules of penicillamine. Penicillamine reduces excess cystine excretion in cystinuria. This is by disulfide interchange between penicillamine and cystine, which results in formation of a readily excreted penicillamine-cysteine disulfide. Penicillamine interferes with the formation of cross-links between tropocollagen molecules and cleaves them when newly formed. Penicillamine lowers IgM rheumatoid factor and depresses T-cell activity.
+4°C

Supplier: Invitrogen

Thermo Scientific Pierce Sulfo-SDA (Sulfo-NHS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 3.9Å spacer arm separates the two photoreactive groups.

Supplier: Invitrogen

Thermo Scientific Pierce Sulfo-LC-SDA (Sulfo-NHS-LC-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 12.5Å spacer arm separates the two photoreactive groups.

Supplier: MP Biomedicals

Ammonium sulfate is an inorganic salt prepared by treating ammonia with sulfuric acid. Ammonium sulfate decomposes upon heating above 250 °C, forming ammonium bisulfate.

Supplier: MP Biomedicals

Deoxycholic Acid Sodium Salt Monohydrate is an anionic biological detergent for the solubilization of membrane-bound proteins.

Supplier: Invitrogen

Thermo Scientific Pierce TCEP-HCl is a potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. The unique compound is easily soluble and very stable in many aqueous solutions. TCEP reduces disulfide bonds as effectively as dithiothreitol (DTT), but unlike DTT and other thiol-containing reducing agents, TCEP does not have to be removed before certain sulfhydryl-reactive crosslinking reactions.

Supplier: Invitrogen

Thermo Scientific Pierce SDAD (NHS-SS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 13.5Å spacer arm containing a cleavable disulfide bond separates the two photoreactive groups.

Supplier: Invitrogen

Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

Supplier: Invitrogen

Thermo Scientific Pierce LC-SDA (NHS-LC-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 12.5Å spacer arm separates the two photoreactive groups.

Supplier: Invitrogen

Thermo Scientific Pierce LC-SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long cyclohexane-stabilized spacer arm (16.2 angstroms).

Supplier: Invitrogen

Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

Supplier: MP Biomedicals

Paromomycin Sulfate is an aminoglycoside antibiotic which inhibits protein initiation.

Supplier: STEMCELL Technologies

DAPI (4',6-diamidino-2-phenylindole dihydrochloride) is a blue-fluorescent dye that binds to AT-rich regions of double-stranded DNA.

Supplier: BIOASSAY SYSTEMS

For quantitative determination of DNA.

Supplier: BENTO BIOWORKS LTD SE

The mobile lab that includes a thermal cycler, powered electrophoresis, blue light transilluminator, and centrifuge, all into one unit.

Supplier: Invitrogen

Thermo Scientific Pierce Sulfo-SDAD (Sulfo-NHS-SS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 13.5Å spacer arm containing a cleavable disulfide bond separates the two photoreactive groups.

Supplier: Invitrogen

Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.

Supplier: Invitrogen

Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.