914 Results for: "Molecular Biology Reagents"

Supplier: Cytiva

Sodium pyruvate solution is an easily accessible carbohydrate source that may also have protective effects against hydrogen peroxide.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C).
D-myo-Inositol is a component of membrane phospholipids, glycosylphophatidylinositol anchors that bind glycoproteins to cell membranes, and inositol phosphate second messengers. It is a growth factor for animals as well as microorganisms. Myo-Inositol is the most abundant form of polyols that serves as a structural element of secondary messengers in eukaryotic cells. Inositol or cyclohexane-1,2,3,4,5,6-hexol is a chemical compound, a sixfold alcohol (polyol) of cyclohexane. It exists in nine possible stereoisomers, of which the most prominent form, widely occurring in nature, is cis-1,2,3,5-trans-4,6-cyclohexanehexol, or myo-inositol (former name meso-inositol). Inositol is a carbohydrate, though not a classical sugar. It has a taste which has been assayed at half the sweetness of table sugar. Used as a lipotropic agent.
A component of membrane phospholipids, glycosyl­phosphatidyl­inositol anchors that bind glycoproteins to cell membranes, and inositol phosphate second messengers. In addition, inositol serves as an important component of the structural lipids phosphatidylinositol (PI) and its various phosphates, the phosphatidylinositol phosphate (PIP) lipids.

Supplier: Cytiva

HyClone™ Penicillin-Streptomycin Solution (100X) is a broad spectrum antibiotic designed to control and destroy destructive microorganisms, such as gram negative and gram positive bacteria.

Supplier: MP Biomedicals

Dimethyl Sulfoxide is ideal for use as a cryoprotectant. Also used in chemical reactions, in polymerase chain reactions (PCR) as a PCR cosolvent to help improve yields, especially in long PCR, and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal-induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines, embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS). Used to enhance dermal absorption of many chemicals. A solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers. Used in antifreeze or hydraulic fluids. A cryopreservative for cell cultures. Used in the oxidation of thiols and disulfides to sulfonic acids.

Dimethyl sulfoxide (DMSO) is a highly polar aprotic organic reagent that has exceptional solvent properties for organic and inorganic chemicals

Physical Apppearance: Liquid
Viscosity: 1.1 centipoises (27 °C)
Vapor Density: 2.7 (vs air)
Vapor Pressure: 0.55 hPa at 20 °C, 0.42 mmHg (20 °C)
Refractive Index: n20/D 1.479(lit.)
Solubility: Soluble in water (1 mL DMSO + 1 mL H2O), methanol, acetone, ether, benzene, chloroform

Supplier: MP Biomedicals

N-Acetyl-L-cysteine is an acetylated amino acid with antioxidant and mucolytic properties for isolation of mycobacteria from sputum. As a mucolytic agent, N-Acetyl-L-cysteine serves to dissipate disulfide bonds across mucoproteins, loosening and clearing the viscosity of sputum.
N-Acetyl-L-cysteine acts as a free-radical scavenger and inhibits the production of reactive oxygen species which can damage cellular structures. It is critical for the production of Glutathione, a key antioxidant enzyme in the body. It has also been shown to induce apoptosis in vascular smooth muscle cells, indicating that these cells respond differently to changes in reduction-oxidation state than other tissues that are normally protected by the presence of antioxidants.
Antioxidant and mucolytic agent. Increases cellular pools of free radical scavengers. Reported to prevent apoptosis in neuronal cells but induce apoptosis in smooth muscle cells. Inhibits HIV replication. May serve as a substrate for microsomal glutathione transferase.

Supplier: Invitrogen

Thermo Scientific EZ-Link Hydrazide-PEG4-Biotin (also called Biotin-PEG4-Hydrazide) is an aldehyde-reactive biotinylation reagent that contains a 4-unit polyethylene glycol (PEG) spacer arm for increased hydrophilicity.

Supplier: MP Biomedicals

Aprotinin is a single chain polypeptide (58 amino-acids) crosslinked by three disulfide bridges, showing competitive and reversible inhibiton of proteolytic and esterolytic activity. Aprotinin forms stable complexes with, and blocks the active sites of serine protease enzymes. It is found in bovine lymph nodes, lung, parotid gland, spleen, liver, pancreas, seminal vesicles, thyroid gland, kidney, mucous membranes of the trachea and esophagus, ovaries, heart, posterior pituitary and cartilage.

Supplier: Invitrogen

Thermo Scientific EZ-Link Hydrazide-LC-Biotin is a mid-length, simple, hydrazide-activated biotinylation reagent for labeling glycoproteins and other carbohydrate-containing compounds having oxidizable sugars or aldehydes.

Supplier: Invitrogen

Thermo Scientific Pierce Sulfo-SANPAH is a hetero-bifunctional crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.

Supplier: MP Biomedicals

PMA (Phorbol-12-Myristate-13-Acetate) is a diester of phorbol and is a tumor promoting compound extracted from croton oil. It is a reversible, highly potent protein kinase C (PKC) activator in vitro and in vivo at nM concentrations. PMA’s effects on PKC are attributed to its similarity to diacylgylerol, a natural activator of PKC.
PMA activates Ca2+- ATPase and potentiates forskolin-induced cAMP formation. It has been shown to inhibit apoptosis induced by the Fas antigen, but PMA induces apoptosis in HL-60 promyelocytic leukemia cells.
Potent tumor promoter; activates protein kinase C in vivo and in vitro.
Store at -20 °C. Protect from light.

Supplier: Thermo Scientific Chemicals

Silver protein is used in electron microscopy with periodic acid and thiocarbohydrazide or thiosemicarbohydrazide as a positive stain for carbohydrates such as glycogen. It can also be used for light microscopy to stain nerve tissue. It is also used in histological stain for microscopic analysis.

Supplier: Adipogen

Popular green fluorescent cell-permeable dye. Covalently couples via its succinimidyl group to intracellular molecules. The amine-reactive succinimidyl ester of 6-FAM acid is a common fluorescent derivatization reagent for covalently labeling peptides, proteins and nucleotides. Due to this covalent coupling reaction it can be retained within cells for extremely long periods and due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. Yields carboxyamides which are more resistant to hydrolysis than fluorescein conjugates prepared with FITC. Better retained in cells than fluorescein. Spectral data: lambdaex 496 nm| lambdaem 516 nm (Buffer pH 9.0).

Supplier: Invitrogen

Thermo Scientific Pierce DSG is a water-insoluble, homo-bifunctional N-hydroxysuccinimide ester (NHS-ester) crosslinker often used for conjugating radiolabeled ligands to cell-surface receptors. The NHS ester is the simplest and most commonly used reactive group for crosslinking and labeling proteins and peptides. NHS esters react with primary amines on the N-termini of peptides and the  amine of lysine residues, forming a stable, covalent amide bond and releasing the NHS group.

Supplier: Biotium

5(6)-CFDA, SE (full name: 5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester, also known as CFSE) is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling.

Supplier: Invitrogen

Thermo Scientific Pierce Premium Grade Sulfo-NHS is our highest quality formulation of this N-hydroxysuccinimide chemical modification reagent, specially characterized for applications where product integrity and risk minimization are paramount.

Supplier: GeneMate

Taq DNA Polymerase is a thermostable DNA polymerase, which can withstand prolonged incubation at high temperatures without a significant loss of activity

Supplier: MP Biomedicals

Polymyxin B Sulfate is a mixture of Polymyxin B1 and Polymyxin B2 Sulfate, obtained from Bacillus polymyxia strains. Polymyxin B inhibits gram-negative organisms in vitro and is also reported to inhibit Ca2+ dependent protein kinase C (PKC). It binds to and interferes with the permeability of the bacterial cytoplasmic membrane of gram-negative bacteria.

Supplier: MP Biomedicals

Saponin is "built" from quillaic acid, a sugar and possibly other substances. It is a Glycoside widely distributed in plants.

Supplier: Invitrogen

Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

Supplier: MP Biomedicals

Storage: -20 °C.
Adenosine 5'-triphosphate (ATP) and its phosphate bonds are the basic components of energy exchange in many biological systems.
Adenosine 5'-triphosphate (ATP) is a central component of energy storage and metabolism in vivo. ATP is used in many cellular processes, respiration, biosynthetic reactions, motility, and cell division. ATP is a substrate of many kinases involved in cell signaling and of adenylate cyclase(s) that produce the second messenger cAMP. ATP provides the metabolic energy to drive metabolic pumps. ATP serves as a coenzyme in a wide array of enzymatic reactions.
P2 purinergic agonist; increases activity of Ca2+-activated K+ channels; substrate for ATP-dependent enzyme systems.

Supplier: MP Biomedicals

SDS is an anionic detergent and wetting agent that is effective in both acid and alkaline solutions.

Supplier: MP Biomedicals

2-Propanol ACS

Supplier: MP Biomedicals

Storage: Store at +4 °C, store under nitrogen
Glutathione is the major low molecular weight thiol compound of the living plant or animal cell. It is a tripeptide with a gamma peptide linkage between the amine group of cysteine (which is attached by normal peptide linkage to a glycine) and the carboxyl group of the glutamate side-chain. It is an antioxidant, preventing damage to important cellular components caused by reactive oxygen species such as free radicals and peroxides. The sulfhydryl (thiol) group (SH) of cysteine serves as a proton donor and is responsible for the biological activity of glutathione.
Glutathione suppresses human immunodeficiency virus expression in chronically infected monocytic cells. It is a useful tripeptide involved in many aspects of metabolism, including transport of g-glutanyl amino acids and reductive cleavage of disulfide bonds.
Endogenous antioxidant that plays a major role in reducing reactive oxygen species formed during cellular metabolism and the respiratory burst. Glutathione-S-transferase catalyzes the formation of glutathione thioethers with xenobiotics, leukotrienes, and other molecules that have an electrophilic center. Glutathione also forms disulfide bonds with cysteine residues in proteins. Via these mechanisms, it can have the paradoxical effect of reducing the efficacy of anti-cancer agents.

Supplier: MP Biomedicals

Potassium phosphate is a reagent with high buffering capacity. It occurs in several forms: monobasic, dibasic and tribasic. Most pH neutral potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH.

Supplier: Promega Corporation

The PolyATtract System isolates messenger RNA directly from total RNA.

Supplier: Invitrogen

Thermo Scientific Pierce Trifluoroacetic Acid (TFA), Sequencing grade is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.

Supplier: MP Biomedicals

HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. Its dissociation decreases as the temperature decreases which makes it a more effective buffering agent for maintaining enzyme structure and function at low temperatures.

Supplier: Invitrogen

Thermo Scientific™ Pierce™ Premium Grade Sulfo-NHS-SS-Biotin is our highest quality formulation of this amine-reactive, cleavable biotinylation reagent, specially characterized for applications where product integrity and risk minimization are paramount. Sulfo-NHS-SS-biotin is used for cleavable attachment of biotin to protein and cell surface amines.

Supplier: Rockland Immunochemical

5.0 M Potassium Acetate (untitrated) DEPC Treated

Supplier: STEMCELL Technologies

For detection of active caspase-3 in mammalian cells undergoing apoptosis.