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914 results for "Molecular Biology Reagents"

914 Results for: "Molecular Biology Reagents"

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Bovine Aprotinin (from Lung), MP Biomedicals

Supplier: MP Biomedicals

Aprotinin is found in bovine lymph nodes, lung, parotid gland, spleen, liver, pancreas, seminal vesicles, thyroid gland, kidney, mucous membranes of the trachea and esophagus, ovaries, heart, posterior pituitary and cartilage. It is a serine protease inhibitor which is reactive against trypsin, chymotrypsin, plasmatic and glandular kininogenases, plasmin, kallkrein, urokinase, clotting factor XIIa, protein C, proteinases of the complement system, leukocyte and tissue proteinases. It does not inhibit thrombin. Aprotinin works by blocking the active sites of enzymes. Binding is reversible with most aprotinin-protease complexes dissociating at pH > 10 or < 3.

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Sulphosuccinimidyl acetate (Sulfo-NHS-acetate), Pierce™

Sulphosuccinimidyl acetate (Sulfo-NHS-acetate), Pierce™

Supplier: Invitrogen

Pierce™ Sulfo-NHS-Acetate is a small compound used for blocking primary amines (eg

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DST (Disuccinimidyl tartrate), Pierce™

DST (Disuccinimidyl tartrate), Pierce™

Supplier: Invitrogen

Thermo Scientific Pierce DST, also called disuccinimidyl tartrate, is a water-soluble crosslinker that contains amine-reactive NHS ester ends around a 4-atom spacer arm, whose central diol can be cleaved by periodate oxidation.

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Sulfo-NHS-SS-Biotin, EZ-Link™, Pierce™

Sulfo-NHS-SS-Biotin, EZ-Link™, Pierce™

Supplier: Invitrogen

Thermo Scientific EZ-Link Sulfo-NHS-SS-Biotin is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.

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Horse Gamma Globulin Fraction

Supplier: Rockland Immunochemical

Lyophilized Gamma Globulin Fraction

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Rat Gamma Globulin Fraction

Supplier: Rockland Immunochemical

Lyophilized Gamma Globulin Fraction

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Acrylamide ≥99.9%, crystalline powder, Ultrapure for electrophoresis

Supplier: MP Biomedicals

Acrylic Acid Content <0.01%

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ReadiLink™ iFluor® 488 Nick Translation dsDNA Labeling Kit

ReadiLink™ iFluor® 488 Nick Translation dsDNA Labeling Kit

Supplier: AAT BIOQUEST INC

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

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Human Gamma Globulin Fraction

Supplier: Rockland Immunochemical

Lyophilized Gamma Globulin Fraction

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Hamster Gamma Globulin Fraction

Supplier: Rockland Immunochemical

Lyophilized Gamma Globulin Fraction

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Acrylamide ≥99.9%, white crystalline powder

Supplier: MP Biomedicals

Acrylamide is a monomer used in a variety of synthetic processes to form polymers and copolymers. It polymerizes in the presence of free radicals in aqueous solutions. It is usually used to prepare polyacrylamide gels for electrophoresis separation of biomolecules. Common reaction initiators are riboflavin and ammonium persulfate; varying the ratio of acrylamide to crosslinking agent permits the formation of a gel with predictable average pore size and texture. Acrylamide tends to hydrolyze under acidic or basic conditions to form acrylic acid. Polyacrylamide plastics have numerous commercial applications, due in part to the tendency of polyacrylamide to adsorb many times its own mass in water. Acrylic acid and any ionic impurities in the acrylamide can have significant effects on the performance of the acrylamide gel formed, since the voltage across the gel is affected by the ionic charge of the gel and usage buffers.
Store at Room Temperature (15-30 °C), Dessicated.

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AMCA succinimidyl ester ≥90%

AMCA succinimidyl ester ≥90%

Supplier: AAT BIOQUEST INC

AMCA is one of the brightest blue fluorescent labeling dyes.

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Ammonium acetate 7.5 M, clear solution

Ammonium acetate 7.5 M, clear solution

Supplier: MP Biomedicals

Ammonium acetate is a chemical compound which is derived from the reaction of ammonia and acetic acid. Ammonium acetate is volatile at low pressures. Because of which it has been used to replace cell buffers with non-volatile salts, in preparing samples for mass spectrometry. It is used as a buffer for mobile phases for HPLC with ELSD detection.

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2-Mercaptoethanol ≥98%, liquid cell culture reagent

Supplier: MP Biomedicals

Storage: Room Temperature
2-Mercaptoethanol is a hybrid of ethylene glycol and 1,2-ethanedithiol. It is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. It is widely used because the hydroxyl group confers solubility in water and lowers the volatility. Due to its diminished vapor pressure, its odour, while unpleasant, is less objectionable than related thiols.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also it reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

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Gentamicin sulfate USP

Supplier: MP Biomedicals

Gentamicin sulfate is an aminoglycoside antibiotic complex produced by fermentation of Micromonospora purpurea or M

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Random Mutagenesis Kits GeneMorph II, Agilent Technologies

Random Mutagenesis Kits GeneMorph II, Agilent Technologies

Supplier: Agilent Technologies

The GeneMorph II random mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis.

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Sulfo-NHS-LC-Biotin, Premium Grade, Pierce™

Sulfo-NHS-LC-Biotin, Premium Grade, Pierce™

Supplier: Invitrogen

Thermo Scientific Pierce Premium Grade Sulfo-NHS-LC-Biotin is our highest quality formulation of this popular amine-reactive biotinylation reagent, specially characterized for applications where product integrity and risk minimization are paramount.

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Thymidine (2'-deoxythymidine) ≥99% cell culture reagent

Supplier: MP Biomedicals

Thymidine is a nucleoside composed of deoxyribose (a pentose sugar) joined to the pyrimidine base thymine.Deoxythymidine can be phosphorylated with one, two or three phosphoric acid groups, creating respectively dTMP, dTDP or dTTP (deoxythymidine mono- di- or triphosphate.It exists in solid form as small white crystals or white crystalline powder.The stability of deoxythymidine under standard temperature and pressure (STP) is very high.Thymidine is listed as a chemical teratogen.
Mainly used in forming hybrid cell lines wherein cells are incubated in HAT medium(hypoxanthine-aminopterin-thymidine medium) to give rise to monoclonal antibodies. Use in HT and HAT cocktails for hybridoma fusion, selection and cloning.
Physical Appearance: White Powder
Optical Rotation: (c=1, water) +17.5 - +19.5°
UV/Visible Absorbance: λmax (0.1N HCl) 266 - 268 nm
Solubility: Soluble in water, methanol, hot alcohol, hot acetone, hot ethyl acetate pyridine, glacial acetic acid and HCl (20 mg/mL-clear, colorless solution); sparingly soluble in hot chloroform

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Sodium dodecyl sulfate (SDS) 97% for electrophoresis

Supplier: Thermo Scientific Chemicals

Powder

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Protein A, HRP (Horseradish Peroxidase)

Supplier: Rockland Immunochemical

Protein A Peroxidase Conjugated

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Protein G, HRP (Horseradish Peroxidase)

Supplier: Rockland Immunochemical

Protein G Peroxidase Conjugated

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CSM-Arg Powder, MP Biomedicals

CSM-Arg Powder, MP Biomedicals

Supplier: MP Biomedicals

Single dropout of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media.

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DSP (Dithiobis(succinimidyl propionate)) ≥94% (by HPLC), water soluble, Calbiochem®, Sigma-Aldrich®

Supplier: MilliporeSigma

Bifunctional cross-linker. The disulfide bond can be cleaved with 1% NaBH4, 50 mM DTT, or 100 mM β-mercaptoethanol. Has been used to immobilize proteins on supports containing amino groups.

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Penicillin : Streptomycin solution, HyClone™

Penicillin : Streptomycin solution, HyClone™

Supplier: Cytiva

HyClone™ Penicillin-Streptomycin Solution (100X) is a broad spectrum antibiotic designed to control and destroy destructive microorganisms, such as gram negative and gram positive bacteria.

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Spermine tetrahydrochloride ≥96%, white powder

Supplier: MP Biomedicals

Spermine is a naturally occuring polyamine that occurs in all eukaryotes, but is rare in prokaryotes. It is essential for cell growth in both normal and neoplastic tissue.

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Trifluoroacetic acid, Sequencing grade, Pierce™

Trifluoroacetic acid, Sequencing grade, Pierce™

Supplier: Invitrogen

Thermo Scientific Pierce Trifluoroacetic Acid (TFA), Sequencing grade is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.

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Propidium iodide 1mg/ml in aqueous solution

Supplier: Thermo Scientific Chemicals

Propidium iodide, 1mg/ml aqueous soln.

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Dimethyl sulfoxide ≥99% cell culture reagent

Supplier: MP Biomedicals

Dimethyl Sulfoxide is ideal for use as a cryoprotectant. Also used in chemical reactions, in polymerase chain reactions (PCR) as a PCR cosolvent to help improve yields, especially in long PCR, and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal-induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines, embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS). Used to enhance dermal absorption of many chemicals. A solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers. Used in antifreeze or hydraulic fluids. A cryopreservative for cell cultures. Used in the oxidation of thiols and disulfides to sulfonic acids.

Dimethyl sulfoxide (DMSO) is a highly polar aprotic organic reagent that has exceptional solvent properties for organic and inorganic chemicals

Physical Apppearance: Liquid
Viscosity: 1.1 centipoises (27 °C)
Vapor Density: 2.7 (vs air)
Vapor Pressure: 0.55 hPa at 20 °C, 0.42 mmHg (20 °C)
Refractive Index: n20/D 1.479(lit.)
Solubility: Soluble in water (1 mL DMSO + 1 mL H2O), methanol, acetone, ether, benzene, chloroform

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Chenodeoxycholic acid

Supplier: Thermo Scientific Chemicals

Bile acid that induces apoptosis through protein kinase C signaling pathways

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Doxorubicin hydrochloride

Supplier: Thermo Scientific Chemicals

Doxorubicin hydrochloride is an antitumor antibiotic agent that inhibits DNA topoisomerase II

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