914 Results for: "Molecular Biology Reagents"
Potassium chloride solution 3M, CellPro™
Supplier: Alkali Scientific
CellPro™ Potassium Chloride Solution stands as a trusted reagent in molecular biology research. Crafted to meticulous standards of purity and sterility, this solution offers researchers a reliable source of potassium ions for experimental procedures. With its sterile filtered formulation, CellPro™ Potassium Chloride Solution ensures consistent and reproducible results, empowering researchers in their molecular biology experiments.
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Water, CellPro™ nuclease-free, distilled
Supplier: Alkali Scientific
CellPro™ Distilled Water stands as a reliable reagent for molecular biology applications. Crafted to meticulous standards of purity, this water is nuclease-free, ensuring minimal interference with nucleic acid-related experiments. With its high-quality formulation, CellPro™ Distilled Water provides researchers with confidence in the integrity of their experimental procedures, facilitating successful outcomes in molecular biology studies.
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Cetrimonium bromide ∼98% for molecular biology
Supplier: MP Biomedicals
Cetyltrimethylammonium bromide is a phase transfer catalyst ideal for the precipitation of DNA. Hexadecyltrimethylammonium bromide is a bactericidal, cationic detergent. Its activity is neutralized by soaps and anionic detergents (i.e., SDS). Trimethylammonium bromide compounds form insoluble complexes with SDS. It is active at alkaline pH against both Gram positive and Gram negative organisms.
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Sodium dihydrogen phosphate 1M, CellPro™
Supplier: Alkali Scientific
CellPro™ Sodium Phosphate Solution offers researchers a high-quality reagent for molecular biology applications. Crafted to stringent standards of purity and sterility, this solution ensures reliable performance in experimental procedures. With its sterile filtered formulation, CellPro™ Sodium Phosphate Solution provides researchers with confidence and peace of mind in their work, facilitating successful outcomes in molecular biology studies.
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Dithiothreitol (DTT, Cleland's reagent)
Supplier: IBI Scientific
DTT is used in enzyme preparations to prevent formation of disulfides from sulfhydryl groups, thereby stabilizing the enzyme.
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HEPES sodium salt ≥99.0% for molecular biology
Supplier: MP Biomedicals
HEPES is a zwitterionic organic chemical buffering agent.
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EDTA disodium salt dihydrate ≥99.5%
Supplier: IBI Scientific
EDTA is commonly used in biological and electrophoresis buffer systems.
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Disodium hydrogen phosphate solution 1M, CellPro™
Supplier: Alkali Scientific
CellPro™ Sodium Phosphate Solution offers researchers a high-quality reagent for molecular biology applications. Crafted to stringent standards of purity and sterility, this solution ensures reliable performance in experimental procedures. With its sterile filtered formulation, CellPro™ Sodium Phosphate Solution provides researchers with confidence and peace of mind in their work, facilitating successful outcomes in molecular biology studies.
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Orthoboric acid ≥99.5%
Supplier: IBI Scientific
Boric Acid is an ingredient in Tris-Borate buffer systems used in high voltage electrophoresis.
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Chicken Lysozyme Type VI (from Egg White), MP Biomedicals
Supplier: MP Biomedicals
Lysozyme (muramidase) hydrolyzes preferentially the b-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus.
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TRITON™ X-100 (Polyethylene glycol tert-octylphenyl ether) DNase-, RNase-, protease-free
Supplier: IBI Scientific
Triton X-100 is a non-ionic detergent used to denature cell membranes without denaturing the protein. Triton X-100 breaks up protein aggregates to promote enzymatic activity.
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β-(N-Morpholino)ethanesufonic acid (MES) monohydrate ≥99%, white crystalline powder for molecular biology
Supplier: MP Biomedicals
MES is a zwitterionic buffer. One of the "Good" buffers developed for biological applications. It has the advantages of maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pK with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range.
A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa ± 1). Alternatively, volumes of equimolar MES free acid and sodium or potassium MES can be mixed to attain the desired pH.
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Mineral oil light, liquid for molecular biology
Supplier: MP Biomedicals
Mineral Oil is a colorless, odorless mixture of alkanes from a mineral source. It is often used as an inert overlay to minimize evaporation in molecular biology applications, including PCR, but has many other applications on other research fields.
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Sodium acetate 3 M in aqueous solution, sterile filtered, pH 5.2
Supplier: QUALITY BIOLOGICAL, INC.
Sodium Acetate, 3M pH 5.2 is a common molecular biology reagent. All production lots are tested to ensure that they are free of Dnase, Rnase & Proteases. It is often used in buffer systems to maintain the pH of a given solution.
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Acrylamide ≥99%, white crystalline powder for molecular biology
Supplier: MP Biomedicals
Storage: Room Temperature, desiccate.
Acrylamide is prepared on an industrial scale by the hydrolysis of acrylonitrile by nitrile hydratase.
Acrylamide is a monomer used in a variety of synthetic processes to form polymers and copolymers. It polymerizes in the presence of free radicals in aqueous solutions. It is usually used to prepare polyacrylamide gels for electrophoresis separation of biomolecules.
Acrylamide is an extremely hazardous substance in either the powder form or liquid form. Once polymerized, the polyacrylamide is considered safe to handle under normal laboratory conditions. Acrylic Acid Content -≤0.001%
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Ethidium bromide 10 mg/mL in aqueous solution for molecular biology
Supplier: IBI Scientific
Used to detect both single- and double-stranded nucleic acids (both DNA and RNA). Does not intercollate into single-stranded DNA very well; therefore, the fluorescence of single-stranded DNA is relatively poor in comparison to double-stranded DNA. The recommended final concentration of ethidium bromide in agarose gels and electrophoresis buffers is 0.5µg/mL.
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Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)
Supplier: RPI
TRIS Amino Ultra PC is a specialized reagent utilized particularly in molecular biology and biochemistry. It is a high-quality, ultra-pure form of TRIS (tris(hydroxymethyl)aminomethane), a commonly used buffering agent in biological applications.
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Guanidinium hydrochloride
Supplier: IBI Scientific
Guanidine Hydrochloride is an ionic denaturant that rapidly and effectively denatures most proteins
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9-Anthrealdehyde ≥99% (by HPLC)
Supplier: Adipogen
Fluorescent reagents (probe, stain, label) with reactive functional group for chromatography or spectroscopy. Derivatization agent. Used for membrane research, luminescent detection, molecular biology, protein modifications, protein structural analysis and proteomics. Carbohydrate labels for chromatography.
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Ethanol denatured ≥95%, Biotechnology Grade (denatured with 5% methanol)
Supplier: IBI Scientific
Ethanol is widely used for precipitating nucleic acids. IBI 200 Proof Ethanol is denatured with 5% methanol.
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Disodium hydrogen phosphate heptahydrate ≥98%, white crystalline powder ACS
Supplier: MP Biomedicals
Sodium phosphate dibasic heptahydrate is a reagent with very high buffering capacity widely used in molecular biology, biochemistry and chromatography. Sodium phosphate dibasic is highly hygroscopic and water soluble. Useful in conjunction with Sodium Phosphate, Monobasic in the preparation of biological buffers.
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Phosphate Buffered Saline, 10X
Supplier: RPI
PBS 10X solution, is a widely used reagent in life science research. It is a buffered saline solution that contains phosphate ions, which help maintain a stable pH level in biological and biochemical experiments. PBS 10X solution is commonly used in various applications such as cell culture, immunohistochemistry, immunocytochemistry, and molecular biology techniques. The primary function of PBS 10X solution is to provide a stable environment for biological samples by maintaining a physiological pH and ionic strength.
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IPTG (isopropyl-β-D-thiogalactopyranoside) dioxane-free
Supplier: Adipogen
Molecular biology reagent. beta-Galactosidase activity inducer in several bacteria, by binding and inhibiting the lac repressor. Used in gene cloning together with X-Gal (Prod. No. AG-CC1-0003), in a technique called blue/white screening. Distinguishes between recombinant and non-recombinant plasmids carrying the beta-galactosidase gene. Plant origin, contains no animal-derived contaminants. Stimulates beta-galactosidase in cellular systems in which dioxane would disrupt normal cell function.
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X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)
Supplier: Adipogen
Molecular biology reagent. Chromogenic substrate for beta-galactosidase. Used in gene cloning together with IPTG (Prod. No. AG-CC1-0002) to indicate whether a cell expresses the beta-galactosidase enzyme, which is encoded by the lacZ gene, in a technique called blue/white screening. Distinguishes between recombinant and non-recombinant plasmids carrying the beta-galactosidase gene. Used in two-hybrid analysis. X-Gal is used to determine E. coli and coliform content in drinking water samples.
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Guanidine thiocyanate
Supplier: CHEM-IMPEX INTERNATIONAL, INC
Guanidine thiocyanate is a versatile chemical compound widely utilized in molecular biology and biochemistry, particularly for its role in nucleic acid extraction and purification. This compound acts as a chaotropic agent, effectively disrupting hydrogen bonds and facilitating the denaturation of proteins and nucleic acids. Its unique properties make it an essential reagent in the isolation of RNA and DNA from biological samples, ensuring high yields and purity. Researchers appreciate its efficiency in lysing cells and inactivating RNases, which is crucial for maintaining the integrity of nucleic acids during extraction processes. In addition to its applications in nucleic acid research, guanidine thiocyanate is also employed in various industrial processes, including the synthesis of pharmaceuticals and agrochemicals. Its ability to solubilize proteins and enhance reaction rates makes it a valuable component in formulations requiring protein stabilization. With its broad range of applications and effectiveness in molecular biology, guanidine thiocyanate stands out as a reliable choice for researchers and industry professionals seeking high-quality results in their work.
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Potassium dihydrogen phosphate, anhydrous, white crystalline powder cell culture reagent
Supplier: MP Biomedicals
Storage: Room Temperature, desiccate
Potassium phosphate is a reagent with high buffering capacity. It occurs in several forms: monobasic, dibasic and tribasic (K3PO4). Most pH neutral potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH.
Potassium phosphate monobasic is typically used as a component for a wide variety of media used in the culture of microorganisms. It is widely used in molecular biology, biochemistry, and chromatography. It is a component in phosphate buffered saline (PBS). In addition to helping maintain pH, it supplies essential phosphate.
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Sodium dodecyl sulfate (SDS) 10%, CellPro™
Supplier: Alkali Scientific
Alkali Scientific™ presents CellPro™ SDS, Sodium Dodecyl Sulfate, a high-quality reagent designed for protein denaturation and DNA/RNA extraction applications. With its exceptional purity and consistency, CellPro™ SDS ensures reliable results in molecular biology experiments, including Western blotting, DNA sequencing, and protein analysis. This versatile detergent effectively solubilizes hydrophobic molecules and disrupts non-covalent bonds, making it an essential tool for various laboratory protocols. Trust Alkali Scientific™ CellPro™ SDS for superior performance and reproducibility in your research endeavors.
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Sodium dodecyl sulfate (SDS) 20%, CellPro™
Supplier: Alkali Scientific
Alkali Scientific™ presents CellPro™ SDS, Sodium Dodecyl Sulfate, a high-quality reagent designed for protein denaturation and DNA/RNA extraction applications. With its exceptional purity and consistency, CellPro™ SDS ensures reliable results in molecular biology experiments, including Western blotting, DNA sequencing, and protein analysis. This versatile detergent effectively solubilizes hydrophobic molecules and disrupts non-covalent bonds, making it an essential tool for various laboratory protocols. Trust Alkali Scientific™ CellPro™ SDS for superior performance and reproducibility in your research endeavors.
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Pig Hemin
Supplier: CHEM-IMPEX INTERNATIONAL, INC
Hemin, derived from porcine sources, is a vital compound recognized for its significant role in various biochemical applications. This iron-containing porphyrin is primarily utilized in the synthesis of heme proteins and enzymes, making it essential for research in biochemistry and molecular biology. Its unique structure allows it to participate in electron transfer processes, which is crucial for studies related to cellular respiration and metabolic pathways. Hemin is also employed in the preparation of diagnostic reagents and therapeutic agents, particularly in the treatment of conditions such as porphyria. Moreover, Hemin's ability to stabilize and enhance the activity of certain enzymes makes it a valuable tool in enzymatic assays and biocatalysis. Researchers appreciate its compatibility with various biochemical systems, allowing for versatile applications in both academic and industrial settings. With its proven efficacy and reliability, Hemin stands out as a preferred choice for professionals seeking to explore the intricate workings of biological systems.
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Sodium dihydrogen phosphate monohydrate
Supplier: MP Biomedicals
Sodium phosphate is a reagent with very high buffering capacity that is widely used in molecular biology, biochemistry, and chromatography. Sodium phosphate occurs in several forms: monobasic (NaH2PO4), dibasic (Na2HPO4), and tribasic (Na3PO4). Most neutral sodium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH.
Sodium phosphate monobasic, Monohydrate is used as sequestrant, emulsifier and buffer in foods; as mordant in dyeing; for weighting silk in tanning; in manufacture of enamels, ceramics, detergents, boiler compounds; as fireproofing agent; in soldering and brazing instead of borax; as reagent and buffer in analytical chemistry; cathartic; laxative. A table for preparation of 0.1 M sodium phosphate buffer at 25 °C using various proportions of sodium phosphate monobasic and sodium phosphate dibasic has been published. A study of the effect of freeze-thaw storage cycles on proteins in sodium phosphate and potassium phosphate buffer solutions has been reported. The effect of 5 mM sodium phosphate on the efficacy of electrospray ionization (ESI) ion mobility spectrometry (IMS) analysis has been evaluated. A protocol for the purification of pyrogen-free mouse IgG1 monoclonal antibodies which uses 10 mM sodium phosphate (pH 7.4) has been published. An ion-pairing HPLC method for the analysis of 5-aminosalicylic acid has been reported. A TLC method for separation of nucleotide sugars in the study of glycosyltransferase activity has been published.
Room Temperature, Desiccate