911 Results for: "Molecular Biology Reagents"

Supplier: MP Biomedicals

Storage: Room Temperature, desiccate
Potassium phosphate is a reagent with high buffering capacity. It occurs in several forms: monobasic, dibasic and tribasic (K3PO4). Most pH neutral potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH.
Potassium phosphate monobasic is typically used as a component for a wide variety of media used in the culture of microorganisms. It is widely used in molecular biology, biochemistry, and chromatography. It is a component in phosphate buffered saline (PBS). In addition to helping maintain pH, it supplies essential phosphate.

Supplier: G-Biosciences

G-Biosciences offers a selection of DNase and RNase free buffers designed to ensure high quality research.

Supplier: RPI

Protective reagent for maintaining -SH groups in reduced state.

Supplier: RPI

PBS 10X solution, is a widely used reagent in life science research. It is a buffered saline solution that contains phosphate ions, which help maintain a stable pH level in biological and biochemical experiments. PBS 10X solution is commonly used in various applications such as cell culture, immunohistochemistry, immunocytochemistry, and molecular biology techniques. The primary function of PBS 10X solution is to provide a stable environment for biological samples by maintaining a physiological pH and ionic strength.

Supplier: IBI Scientific

IBI's Proteinase K is a stable >50 mg/ml (powder) highly active proteolytic enzyme that is purified from the mold tritirachium album.

Supplier: IBI Scientific

IPTG is an inducer of β-galactosidase activity in E.coli. It is commonly used in conjunction with X-GAL to detect lac gene expression in cloning applications, thus allowing detection of recombinant molecules.

Supplier: IBI Scientific

Kanamycin monosulfate. CAS RN 25389-94-0. Formula Weight: 582.58. Molecular biology grade reagent. Ash: 1.0% max. Kanamycin B: 5.0% max. Moisture: 4.0% max. pH (1% water): 7.5+-1.0. Potency: 750µg/mg max. 25g.

Supplier: MP Biomedicals

Storage: Room Temperature, desiccate, store under nitrogen.
Dimethyl Sulfoxide is a dipola, aprotic solvent. It has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination.
Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly (A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.
To prepare sterile solutions use a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane.

Supplier: Li-Cor

REVERT total protein stain is a membrane stain that fluoresces at 700 nm. This does not covalently modify sample proteins and therefore does not affect antibody binding or quantification. Total protein stain works with existing western blot workflow, without needing any additional reagents or special gels. Staining takes less than ten minutes and is compatible with both PVDF and nitrocellulose membranes.

Supplier: Adipogen

Molecular biology reagent. beta-Galactosidase activity inducer in several bacteria, by binding and inhibiting the lac repressor. Used in gene cloning together with X-Gal (Prod. No. AG-CC1-0003), in a technique called blue/white screening. Distinguishes between recombinant and non-recombinant plasmids carrying the beta-galactosidase gene. Plant origin, contains no animal-derived contaminants. Stimulates beta-galactosidase in cellular systems in which dioxane would disrupt normal cell function.

Supplier: IBI Scientific

Boric Acid is an ingredient in Tris-Borate buffer systems used in high voltage electrophoresis.

Supplier: MP Biomedicals

Lysozyme (muramidase) hydrolyzes preferentially the b-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus.

Supplier: MP Biomedicals

p-Nitroblue Tetrazolium Chloride is an NADPH-diaphorase substrate that competitively inhibits NOS (nitric oxide synthase) (IC50 = 3-4 μM). In combination with 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and an appropriate reaction buffer, Nitroblue tetrazolium forms a substrate for the colorimetric detection of alkaline phosphatase conjugates in a variety of molecular and cell biology techniques.

Supplier: IBI Scientific

Guanidine Hydrochloride is an ionic denaturant that rapidly and effectively denatures most proteins

Supplier: MP Biomedicals

MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers. It is a structural analog to MES. However, MOPS is a more suitable biological buffer than MES because the pH range is closer to pH 7.4.
Store at Room Temperature (15-30 °C)

Supplier: MP Biomedicals

Proteinase K is a highly active stable endopeptidase with a broad spectrum of action was isolated by E. Merk's Darmstadt Biochemical Research Department in 1970 from a culture filtrate of the fungus, Tritirachium album Limber. This fungus is able to grow on Keratin (e.g., wool, horn particles) as the sole source of carbon and nitrogen. The isolated protease was, therefore, given the K designation.

Supplier: Adipogen

Molecular biology reagent. Chromogenic substrate for beta-galactosidase. Used in gene cloning together with IPTG (Prod. No. AG-CC1-0002) to indicate whether a cell expresses the beta-galactosidase enzyme, which is encoded by the lacZ gene, in a technique called blue/white screening. Distinguishes between recombinant and non-recombinant plasmids carrying the beta-galactosidase gene. Used in two-hybrid analysis. X-Gal is used to determine E. coli and coliform content in drinking water samples.

Supplier: Invitrogen

Pierce Silver Stain for Mass Spectrometry is a complete kit for rapid and ultra-sensitive silver staining of proteins in polyacrylamide gels and efficient destaining of excised gel pieces for mass spectrometry analysis. This kit enables both first-time and experienced users to achieve consistent and reliable staining using high-, low-, and gradient-percentage gels in 1D and 2D formats. The optimized staining method ensures extremely sensitive staining while minimizing covalent crosslinking of protein to the gel matrix, which can inhibit protein recovery. The destaining reagents facilitate complete removal of silver from stained protein bands and maximum protein recovery for subsequent mass spectrometry analysis.

Supplier: Spectrum Chemicals

Sodium Phosphate Monobasic, Monohydrate, Granular, BiotechGrade is a reagent with very high buffering capacity widely used in molecular biology, biochemistry and chromatography. Spectrum offers highly pure reagents suitable for biochemical research and analysis that are absent of inhibitors.

Supplier: IBI Scientific

DTT is used in enzyme preparations to prevent formation of disulfides from sulfhydryl groups, thereby stabilizing the enzyme.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C), dessicate.
Ammonium persulfate is a strong oxidizing agent. It is a radical initiator.
Ammonium persulfate is a polymerization catalyst used with TEMED for polyacrylamide gel formation. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry. Other applications of APS include its use as a reducer and retarder in photography, the manufacture of aniline dyes, electroplating, the decolorizing and deodorizing of oils.

Supplier: MP Biomedicals

Storage: Room Temperature, desiccate.
Acrylamide is prepared on an industrial scale by the hydrolysis of acrylonitrile by nitrile hydratase.
Acrylamide is a monomer used in a variety of synthetic processes to form polymers and copolymers. It polymerizes in the presence of free radicals in aqueous solutions. It is usually used to prepare polyacrylamide gels for electrophoresis separation of biomolecules.
Acrylamide is an extremely hazardous substance in either the powder form or liquid form. Once polymerized, the polyacrylamide is considered safe to handle under normal laboratory conditions. Acrylic Acid Content -≤0.001%

Supplier: G-Biosciences

Molecular biology universal kit, contains 25 ml each of ammonium acetate, calcium chloride, EDTA, lithium acetate, magnesium chloride, potassium acetate, potassium chloride, SDS, sodium chloride, TE, Tris pH 7,0 and Tris pH 8,0

Supplier: Alkali Scientific

Alkali Scientific™ presents CellPro™ SDS, Sodium Dodecyl Sulfate, a high-quality reagent designed for protein denaturation and DNA/RNA extraction applications. With its exceptional purity and consistency, CellPro™ SDS ensures reliable results in molecular biology experiments, including Western blotting, DNA sequencing, and protein analysis. This versatile detergent effectively solubilizes hydrophobic molecules and disrupts non-covalent bonds, making it an essential tool for various laboratory protocols. Trust Alkali Scientific™ CellPro™ SDS for superior performance and reproducibility in your research endeavors.

Supplier: MP Biomedicals

HEPES is a zwitterionic organic chemical buffering agent.
HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media.

Supplier: Alkali Scientific

Alkali Scientific™ presents CellPro™ SDS, Sodium Dodecyl Sulfate, a high-quality reagent designed for protein denaturation and DNA/RNA extraction applications. With its exceptional purity and consistency, CellPro™ SDS ensures reliable results in molecular biology experiments, including Western blotting, DNA sequencing, and protein analysis. This versatile detergent effectively solubilizes hydrophobic molecules and disrupts non-covalent bonds, making it an essential tool for various laboratory protocols. Trust Alkali Scientific™ CellPro™ SDS for superior performance and reproducibility in your research endeavors.

Supplier: MP Biomedicals

PIPES is a zwitterionic N-substituted aminosulfonic acid "Good" buffer.
PIPES is used to buffer glutaraldehyde fixative solutions when fixing plant and/or animal tissue samples. This will help prevent the loss of lipids. It is also used to buffer glutaraldehyde and formaldehyde when fixing fungal zoospores for fluorescence microscopy and electron microscopy. PIPES tends to give optimal results.
Buffers can be made by adding a solution of base to PIPES free acid, titrating to the appropriate pH.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C)
Glycine is a non-essential amino acid. It is only amino acid with no asymmetric carbon and thus is not chiral. It is the major inhibitory neurotransmitter. It is involved in the biosynthesis of the porphyrin rings of hemes and chlorophylls.
Glycine is commonly used in buffer solutions, in electrophoresis and preparative chromatography. A study of the folding of monoclonal antibodies in the presence of glycine and their subsequent purification has been published. The use of glycine in the purification of lipopolysaccharides, lipooligosaccharides, and lipid A has been reported. It is an amino acid for use in cell culture media development applications and existing media formulations. Glycine is commonly used as a component in Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis, a component of Towbin's transfer buffer for Western blots, a buffer substance in cryoenzymology, in osmotic pressure maintenance in isoelectric focusing of erythrocytes, salting-in effect in protein chemistry, and as a buffer component in the coupled phosphatase-kinase reaction for end labelling of restriction fragments. The growth requirements of various microorganisms is reported in the Handbook of Microbiology.
Glycine is a non-chiral amino acid that can be synthesized in the body from the amino acid serine by Serine Hydroxymethyltransferase. Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Supplier: G-Biosciences

Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colored product at 412nm.
Ellman's reagent is very useful as a free sulfhydryl assay reagent due to its high specificity for -SH groups at neutral pH, high molar extinction coefficient and short reaction time.

Supplier: MP Biomedicals

Polyethylene glycol (PEG) is a condensation polymer of ethylene oxide and water.
Polyethylene Glycol has been used in the precipitation of proteins, as a fusing agent in enhancing the effect of macrophages on hybridoma, in the separation and purification of biomolecules and in induction of cell hybridization.
PEG's are susceptible to oxidative degradation in the presence of air. Minimizing the exposure of PEG to elevated temperatures and/or exposure to oxygen, or addition of an antioxidant can limit the amount of degradation. PEGs do not hydrolyze or deteriorate upon storage. PEGs do not support the growth of molds.