914 Results for: "Molecular Biology Reagents"

Supplier: MilliporeSigma

10gm. 1,4-Dithiothreitol. DTT. White solid. Purity: >=99% titration. Contaminants: DNase, protease, RNase: none detected. Absorbance (100mM, 1 cm, 260 nm): <=0.500; (100mM, 1 cm, 280 nm): <=0.100. Heavy metals: <=0.001%. RTECS EK1610000, CAS 3483-12-3.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C)
Glycine is a non-essential amino acid. It is only amino acid with no asymmetric carbon and thus is not chiral. It is the major inhibitory neurotransmitter. It is involved in the biosynthesis of the porphyrin rings of hemes and chlorophylls.
Glycine is commonly used in buffer solutions, in electrophoresis and preparative chromatography. A study of the folding of monoclonal antibodies in the presence of glycine and their subsequent purification has been published. The use of glycine in the purification of lipopolysaccharides, lipooligosaccharides, and lipid A has been reported. It is an amino acid for use in cell culture media development applications and existing media formulations. Glycine is commonly used as a component in Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis, a component of Towbin's transfer buffer for Western blots, a buffer substance in cryoenzymology, in osmotic pressure maintenance in isoelectric focusing of erythrocytes, salting-in effect in protein chemistry, and as a buffer component in the coupled phosphatase-kinase reaction for end labelling of restriction fragments. The growth requirements of various microorganisms is reported in the Handbook of Microbiology.
Glycine is a non-chiral amino acid that can be synthesized in the body from the amino acid serine by Serine Hydroxymethyltransferase. Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Supplier: MP Biomedicals

Storage: Room Temperature, desiccate, store under nitrogen.
Dimethyl Sulfoxide is a dipola, aprotic solvent. It has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination.
Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly (A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.
To prepare sterile solutions use a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane.

Supplier: MP Biomedicals

DL-Dithiothreitol is a Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.

Supplier: MP Biomedicals

Glycerol is a simple polyol compound which is a viscous liquid that is widely used in pharmaceutical formulations. It has three hydroxyl groups that are responsible for its solubility in water and its hygroscopic nature.It is a trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, sweetening agent and is of low toxicity.

Supplier: Thermo Scientific Chemicals

Dithiothreitol (DTT, Cleland's reagent) 99+% for molecular biology DNase-, RNase-, protease-free

Supplier: MP Biomedicals

Agarose is a linear polymer consisting of alternating D-galactose and 3,6-anhydro-L-galactose units. Agarose is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marinealgae.

Supplier: Thermo Scientific Chemicals

Liquid

Supplier: Thermo Scientific Chemicals

Sodium acetate, 99+%, anhydrous, Molecular Biology Reagent

Supplier: Thermo Fisher Scientific

Used as a protective agent for SH groups.

Supplier: MilliporeSigma

FICOLL® 400 reagent is useful for the separation of lymphocyte subpopulations.

Supplier: AVIDITY SCIENCE

Replacement cartridges for Millipore lab water systems from a reputable, ISO 9001:2015 certified US manufacture.

Supplier: RPI

Protective reagent for maintaining -SH groups in reduced state.

Supplier: G-Biosciences

Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colored product at 412nm.
Ellman's reagent is very useful as a free sulfhydryl assay reagent due to its high specificity for -SH groups at neutral pH, high molar extinction coefficient and short reaction time.

Supplier: IBI Scientific

CAS RN 69-52-3. Formula Weight 371.39. Molecular Biology Grade Reagent. Moisture: 2% max. pH of a 1% solution: 9.0 +/- 1.0. Potency: 900 ug/mg. 25g.

Supplier: MP Biomedicals

Potassium chloride, Purity: >/=99%, Molecular Biology Reagent, CAS Number: 7447-40-7, Molecular Formula: KCl, Molecular Weight: 74.55, Synonyms: Potassium Monochloride, Sylvite, Enseal, Kaysiel, Slow-K, Color: White, Form: Crystalline Powder, size: 500 g

Supplier: IBI Scientific

Kanamycin monosulfate. CAS RN 25389-94-0. Formula Weight: 582.58. Molecular biology grade reagent. Ash: 1.0% max. Kanamycin B: 5.0% max. Moisture: 4.0% max. pH (1% water): 7.5+-1.0. Potency: 750µg/mg max. 25g.

Supplier: Corning

The 5 M NaCl is a common molecular biology reagent.

Supplier: MP Biomedicals

Ammonium, Biochemicals, Biological Buffers, Buffer / Buffer Salts, Buffers / Buffer Salt, Chemical Synthesis, Inorganic Salts, Life Science Reagents for Cloning, Life Science Reagents for PCR, Life Science Reagents for Protein Expression and Purification, Molecular Biology, Molecular Biology Reagents, Research Essentials, Synthetic Reagents

Supplier: QUALITY BIOLOGICAL, INC.

EDTA (Ethylenediaminetetraacetic acid), a metal chelator, is widely used in molecular biology as a chemical reagent to reduce nuclease activity (i.e. metalloenzyme). EDTA solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and reagents, inorganic salts, and chelators.

Supplier: Invitrogen

Thermo Scientific Pierce Tris is a standard reagent for preparation of many molecular biology buffers.

Supplier: MP Biomedicals

Sodium phosphate is a reagent with very high buffering capacity that is widely used in molecular biology, biochemistry, and chromatography.

Supplier: Corning

Molecular biology grade water is ideal for a wide variety of routine tasks requiring a high degree of sterility Molecular biology grade water is ideal for the preparation of reagents, rinsing glassware and plasticware, and other molecular biology applications where RNase-, DNase-, and Protease-free water is required. No toxic agents, including DEPC, are used in the preparation of Mediatech's molecular biology grade water, eliminating possible interferences with enzymatic reactions.

Supplier: MP Biomedicals

Storage: Store at -20 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X−Gal, is a histochemical substrate for β−galactosidase.
5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X−Gal is cleaved by β−galactosidase to yield an insoluble blue precipitate. X−Gal is particularly useful in molecular biology applications to detect the activity of β−galactosidase which is frequently used as a reporter gene. In cloning, X−Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase.

Supplier: MP Biomedicals

Sodium phosphate monobasic is a reagent with very high buffering capacity widely used in molecular biology, biochemistry and chromatography.

Supplier: Lonza

AccuGENE™ Molecular Biology Buffers are ready-to-use solutions ideal for a wide range of molecular biology applications. Each reagent is prepared with 18 megOhm water, filtered using a 0.2 micron filter, and filled into sterile bottles.

Supplier: IBI Scientific

Glycerol is used to prevent freezing during enzyme and bacterial preparations.

Supplier: MP Biomedicals

HEPES is a zwitterionic organic chemical buffering agent.
HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media.

Supplier: Alkali Scientific

CellPro™ Neutralizing Solution is an essential reagent in molecular biology and biochemical experiments, particularly in protocols involving nucleic acid hybridization and blotting techniques.

Supplier: MP Biomedicals

PIPES is a zwitterionic N-substituted aminosulfonic acid "Good" buffer.
PIPES is used to buffer glutaraldehyde fixative solutions when fixing plant and/or animal tissue samples. This will help prevent the loss of lipids. It is also used to buffer glutaraldehyde and formaldehyde when fixing fungal zoospores for fluorescence microscopy and electron microscopy. PIPES tends to give optimal results.
Buffers can be made by adding a solution of base to PIPES free acid, titrating to the appropriate pH.