77899 Results for: "LC-MS"

Supplier: Invitrogen

Pierce Silver Stain for Mass Spectrometry is a complete kit for rapid and ultra-sensitive silver staining of proteins in polyacrylamide gels and efficient destaining of excised gel pieces for mass spectrometry analysis. This kit enables both first-time and experienced users to achieve consistent and reliable staining using high-, low-, and gradient-percentage gels in 1D and 2D formats. The optimized staining method ensures extremely sensitive staining while minimizing covalent crosslinking of protein to the gel matrix, which can inhibit protein recovery. The destaining reagents facilitate complete removal of silver from stained protein bands and maximum protein recovery for subsequent mass spectrometry analysis.

Supplier: Gold Standard Diagnostics

The AIX1000™ is the first FDA cleared, fully automated system for nontreponemal RPR syphilis testing

Supplier: Thermo Fisher Scientific

The Barnstead MicroPure water purification systems provides up to 15L/day of Type 1, ultrapure (18.2 megohm) water from pre-treated feed water. All MicroPure systems include:feed water monitoring to protect your consumables, quick discconnects to make changing consumables easy, and all core consumables and tubing. MicroPure-ST systems come standard with a 6 liter integrated feed tank for labs that do not have a ready supply of pre-treated feed water.

Supplier: Thermo Fisher Scientific

Accurately monitor conductivity, TDS, salinity, resistivity, and temperature with the Orion™ Star™ A322 Conductivity Portable Meter for challenging field tests.

Supplier: VWR

The VWR® PCR thermal cycler XT⁹⁶ combines industry-inspired innovation with the reliable quality of German manufacturing – housing both in a compact, low noise ventilation design that fits on virtually any bench top. For optimising PCR's, the XT⁹⁶ is also available with a gradient function.
The VWR® PCR thermal cycler XT⁹⁶ offers a powerful, yet easy to use software interface, as well as a host of other innovative features.

Supplier: Prosci

Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1, IL-1Ra and IL-18 (1,2). Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling (1,3) and IL-1R accessory protein (IL-1RAcP) (4). Receptor binding of IL-33 activates NF-B and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine (2). Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties (5). This recombinant protein represents the cleaved and presumably activated form of IL-33, but has not been tested in any biological assays.

Supplier: Prosci

The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. MyD88 has been identified as an adapter molecule in the IL-1 signaling pathway (1). MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-B activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor (2,3), which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.

Supplier: Alkali Scientific

Prepare your buffers easily with QuickSilver™ buffer powder, ensuring high-quality solutions for your laboratory experiments.

Supplier: Thermo Fisher Scientific

The Barnstead GenPure xCAD Plus water purification systems provides up to 200L/day of Type 1, ultrapure (18.2 megohm) water from pre-treated feed water

Supplier: Thermo Fisher Scientific

The Orion™ Pro Star PC215 pH/ion and conductivity bench meter is designed to provide accurate, reliable electrochemistry testing. Featuring an intuitive interface, enhanced data reporting and robust functionality, the Orion™ Pro Star PC215 meter delivers advanced performance in a modern and simplified package.

Supplier: Thermo Fisher Scientific

Get premium performance and flexibility with the Orion™ Versa Star Pro™ Meter with Conductivity Module for conductivity, TDS, salinity, resistivity, and temperature.

Supplier: Omega Bio-Tek

Purify endotoxin free (<0.1 EU/µg) plasmid DNA using magnetic beads from up to 50 ml culture volume.

Supplier: Prosci

Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA (1). Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals, but it is in birds that all subtypes can be found (2). These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. HA interacts with host cell surface proteins containing oligosaccharides with terminal sialyl residues. Its extracellular region has two domains (HA1 and HA2); HA1 is cleaved from the main hemagglutinin protein by the host immune system. During 1997, an H5N1 avian influenza virus was determined to be the cause of death in 6 of 18 infected patients in Hong Kong (3). This more recent virulent strain of H5N1 is now seen in Africa and Europe, as well as in Southeast Asia. There is some evidence of human to human spread of this virus, but it is thought that the efficiency of this type of transmission is low (4). Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species-jumping ability (5). This HA1 recombinant protein is recognized by several ProSci hemagglutinin antibodies.

Supplier: Thermo Fisher Scientific

The Thermo Scientific™ Orion™ Pro Star MP218 pH/ion, conductivity, and dissolved oxygen bench meter is designed to provide accurate, reliable electrochemistry testing. Featuring an intuitive interface, enhanced data reporting and robust functionality, the Orion™ Pro Star MP218 meter delivers advanced performance in a modern and simplified package.

Supplier: General Separation Technologies, Inc.

Typical Application: Acidic/neutral drugs, alkaloids, amines and nitriles, amphetamine and methamphetamine, antiepileptic, basic drugs, chlorinated pesticides, EPA method 508, CLP standard, semivolatile organics, diesel fuel, drug of abuse, endocrine disruptors: alkyl phenols, endocrine disruptors: phthalate, EPA 608.1, EPA air analysis method TO-15, EPA method 525.2, EPA method 551.1, EPA method 610, EPA method 8061 (phthalate ester), EPA method 8270, EPA-625 phenols, flavor mixture, food packaging volatiles, formaldehyde, 50ppb, fragrance allergens, gasoline, halogenated compounds, local anesthetics, nitrogen/phosphorus containing pesticides, EPA 507, organochlorine pesticides, organochlorine pesticides II EPA method 8081A, organohalide pesticides in water, EPA 505, organophosphorous pesticides I EPA 8141A, phenols, I and II, polybrominated diphenyl esters (PBDE), polyethyleneamines, polynuclear aromatic hydrocarbons (PAHs), semivolatile compounds, semivolatile organics, substituted anilines, sulfur in air, trace active amines, 10 ng on-column, tricyclic antipsychotics, urine drug screen, US EOA method 8270D mix.

Supplier: Omega Bio-Tek

The Mag-Bind® Endo-free Plasmid Mini Kit is designed to deliver high-quality, plasmid DNA with endotoxin levels <0.1 EU/μg for use in eukaryotic transfections and other sensitive in vitro applications.

Supplier: Bulldog Bio

PNGase F PRIME is a mutant recombinant PNGase F cloned from Flavobacterium meningosepticum and expressed and purified from E. coli. The proprietary changes made to PNGase F have been shown to have unique characteristics when compared to other commercially-available sources of PNGase F. Data generated by independent labs shows that PRIME works on native glycoproteins and serum glycoproteins in minutes at room temperature. Glycan analysis of the digestion products shows that PNGase F PRIME digestion led to more complete glycan release and also allowed for the cleavage of glycans not released by the commercially-available enzymes when used at the same concentrations with the same digestion conditions. This advancement benefits applications that seek to understand glycobiology in a natural milieu. Preliminary data indicates that PNGase F PRIME has a higher specificity towards complex (tri and tetra-antennary) sialylated structures compared to the commercially sourced enzyme. Additionally, the work presented in this Analytical Chemistry paper utilized PNGase F PRIME for all in situ tissue work as the commercially-available PNGase enzymes did not work on native tissue to allow glycan recognition.

Supplier: Bulldog Bio

PNGase F PRIME is a mutant recombinant PNGase F cloned from Flavobacterium meningosepticum and expressed and purified from E. coli. The proprietary changes made to PNGase F have been shown to have unique characteristics when compared to other commercially-available sources of PNGase F. Data generated by independent labs shows that PRIME works on native glycoproteins and serum glycoproteins in minutes at room temperature. Glycan analysis of the digestion products shows that PNGase F PRIME digestion led to more complete glycan release and also allowed for the cleavage of glycans not released by the commercially-available enzymes when used at the same concentrations with the same digestion conditions. This advancement benefits applications that seek to understand glycobiology in a natural milieu. Preliminary data indicates that PNGase F PRIME has a higher specificity towards complex (tri and tetra-antennary) sialylated structures compared to the commercially sourced enzyme. Additionally, the work presented in this Analytical Chemistry paper utilized PNGase F PRIME for all in situ tissue work as the commercially-available PNGase enzymes did not work on native tissue to allow glycan recognition.

Supplier: Rockland Immunochemical

Mouse IgG TrueBlot® is a unique DyLight™ 800 conjugated anti-mouse IgG immunoblotting (second step) reagent. Mouse IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Fluorescent Western Blot data with Mouse IgG TrueBlot®, simply substitute the conventional DL800 anti-mouse IgG blotting reagent with Fluorescent Mouse TrueBlot® Antibody DyLight™ 800 and follow the prescribed protocol for sample preparation and immunoblotting.

Mouse IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Fluorescent Mouse TrueBlot® Antibody DyLight™ 800 may also be used for detection in immunoassays that do not employ immunoprecipitation.