"Invitrogen"

Supplier: Invitrogen

Thermo Scientific Sodium Dodecyl Sulfate (C12) is a highly purified form of SDS that is especially suited for protein experiments requiring tightly controlled solubility parameters.

Supplier: Invitrogen

Thermo Scientific EZ-Link Sulfo-NHS-SS-Biotin is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.

Supplier: Invitrogen

Thermo Scientific SuperBlock™ Blocking Buffer is optimised solutions in PBS or TBS containing a protein that provides incredibly fast and effective blocking for ELISA, IHC and Western blot analysis.

Supplier: Invitrogen

Pierce™ HisPur™ Ni-NTA Spin Columns contain a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.

Supplier: Invitrogen

Thermo Scientific Pierce Ethidium Bromide is available as a dilute and concentrated solution to accommodate most molecular biology applications for fluorescent DNA visualization.

Supplier: Invitrogen

Hydrazide-activated resin to immobilize glycoproteins for affinity purification.

Supplier: Invitrogen

Thermo Scientific Pierce High Capacity Streptavidin Agarose is a beaded resin of immobilized recombinant streptavidin protein that binds biotinylated proteins at greater than 10 mg/mL of beads.

Supplier: Invitrogen

Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.

Supplier: Invitrogen

Leupeptin Protease Inhibitor reversably inhibits serine and cysteine proteases.

Supplier: Invitrogen

The Slide-A-Lyzer® MINI Dialysis Device Floats are low-density polyethylene rafts designed to securely hold up to 25 Slide-A-Lyzer® MINI Dialysis Devices to permit array dialysis.

Supplier: Invitrogen

Thermo Scientific Pierce Peptide Retention Standard is a mixture of five c-terminal amide decapeptides that provide reference elution profiles in reverse-phase HPLC.

Supplier: Invitrogen

Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples.Easy-to-use—resin provided in single-use spin column formatImproved protein identifications—protein identifications increased by ≥50% when compared to unfractionated samplesReproducible—elution profiles and fractional resolution vary by less than 20%Optimized—robust procedure for maximal protein identification and peptide recovery while minimizing fractional overlapCompatible—reagents validated with a variety of complex samples, including TMT™-labeled peptidesIn order to enable deep proteome sequencing, it is often necessary to reduce the sample complexity by fractionation in an orthogonal dimension prior to LC/MS analysis. The Pierce High pH Reversed-Phase Peptide Fractionation Kit utilizes high pH reversed-phase chromatography to separate peptides by hydrophobicity and provides excellent orthogonality to low pH reversed-phase LC-MS gradients.The kit has been designed to improve protein identification through the use of a proprietary reversed-phase resin in an easy-to-use spin column format with a highpH fractionation protocol. In contrast to strong cation exchange (SCX) fractionation, the high-pH reversed-phase fractions do not require an additional desalting step before LC/MS analysis.The High pH Reversed-phase Peptide Fractionation Kit includes a high pH buffer (0.1% triethylamine) and twelve spin columns containing pH-resistant reversed-phase resin. Each reversed-phase fractionation spin column enables fractionation of 10–100 µg of peptide sample using a microcentrifuge.Native, phosphorylated, Tandem Mass TagTM (TMTTM)-labeled, and other complex peptide mixture samples can be fractionated using this kit. Combining the search results generated by the individual fractions helps improve protein sequence coverage and increases the number of identified proteins relative to unfractionated samples.Applications:Reducing sample complexity to identify targets of interestConducting systems biology studiesReducing sample complexity to improve quantitation studies

Supplier: Invitrogen

The Thermo Scientific 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems.

Supplier: Invitrogen

The Pierce™ TMB Substrate Kit contains soluble TMB (3,3´,5,5´-tetramethylbenzidine) that is ideal for sensitive ELISA-based applications.

Supplier: Invitrogen

Pierce™ SAT(PEG)₄ (PEGylated N-succinimidyl S-acetylthioacetate) is a sulphhydryl-addition reagent for covalent modification of primary amines.

Supplier: Invitrogen

Deoxycholate is an ionic detergent that is especially useful for disrupting and dissociating protein interactions. Stored in a cool, dry, and well-ventilated area, protected from direct sunlight.