"Invitrogen"
Recombinant Streptavidin, APC (Allophycocyanin), Thermo Scientific
Supplier: Invitrogen
Use to develop in-house non-radioactive assay systems.
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Low Protein Binding Collection Tubes (2.0 ml), Thermo Scientific
Supplier: Invitrogen
Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (2.0 ml) help minimize protein sample loss during sample manipulation and are ideal in applications where processing steps are extensive. Able to tolerate centrifuge speeds up to 20000.
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SMPH (Succinimidyl-6-((beta-maleimidopropionamido)hexanoate), Pierce™
Supplier: Invitrogen
Thermo Scientific Pierce SMPH is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long spacer arm (14.2 angstroms).
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Pierce™ AminoLink™ Affinity Coupling Resin, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilization of antibodies and other proteins through primary amines.
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Poly HRP Dilution Buffer, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce Poly-HRP Dilution Buffer is optimized to ensure optimal performance with Poly-HRP Streptavidin.
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Pierce™ DNA Extraction Buffer, Phenol:Chloroform and Tris Buffer, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce Phenol-Chloroform is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1).
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Pierce™ Renilla Luciferase Flash Assay Kit, Thermo Scientific
Supplier: Invitrogen
The Thermo Scientific Pierce Renilla luciferase flash assay kit provides researchers with a highly sensitive intracellular assay for transcriptional activity of regulatory elements in mammalian whole cell lysate.
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SulfoLink™ Coupling Resin, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific SulfoLink Coupling Resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups for covalent immobilization of cysteine-peptides and other sulfhydryl molecules.
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Pierce™ Sulfhydryl Addition Kit, Thermo Scientific
Supplier: Invitrogen
Introduce free sulfhydryl groups into a protein of interest to allow the modified protein to react with a variety of sulfhydryl-selective products.
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Pierce™ Peroxidase Suppressor, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce Peroxidase Suppressor is a stable, easy-to-use inhibitor of endogenous peroxidase activity from cells and tissues to reduce background signal in HRP-based immunohistochemistry applications.
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Guanidinium hydrochloride, Pierce™
Supplier: Invitrogen
Thermo Scientific Pierce Guanidine-HCl can be easily dissolved and pH-adjusted to any concentration.
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Trifluoroacetic acid solution, Sequencing grade, Pierce™
Supplier: Invitrogen
Thermo Scientific Pierce Trifluoroacetic Acid (TFA), Sequencing grade is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.
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Pierce™ Pegylation Reagents, MS(PEG)n Methyl-PEG-NHS-Esters, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce MS(PEG)n reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) and TMS(PEG)12 is a three-branched, polyethylene glycol compound (3 x 12 PEG units). Both are activated as NHS esters for covalent pegylation of primary amines on proteins (eg. lysines) or assay surfaces.
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Pierce™ HisPur™ Ni-NTA Resin, Thermo Scientific
Supplier: Invitrogen
Pierce™ HisPur™ Ni-NTA Resin is a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.
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Pierce™ Monomeric Avidin Agarose, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce Monomeric Avidin Agarose is ideal for purifying biotinylated proteins, peptides and other molecules.
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Pierce™ MS-Compatible Magnetic IP Kit, protein A/G, Thermo Scientific
Supplier: Invitrogen
The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Protein A/G) provides mass spectrometry-friendly reagents and an optimized protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS analysis.MS-compatible—reagents directly compatible with in-solution peptide digestion, enriched samples contain minimal detergent residuals detected using LC-MSSensitive—procedure successfully enriches low abundance proteins (low ng range)Low background—binding, wash, and elution buffers optimized to minimize enrichment of background proteinsRobust—procedure and reagents have been robustly tested with numerous targets to ensure consistent enrichment of low abundance proteins (ng range) with at least 2 peptides identified per proteinThe Pierce MS-Compatible Magnetic IP Kit (Protein A/G) uses high-quality Pierce Protein A/G Magnetic Beads to provide wider flexibility of antibody capture than either Protein A or G alone
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