864 Results for: "Electrophoresis Buffers"

Supplier: Promega Corporation

250-10,000bp; 13 bands. Can be stored at room temperature. Contains dye marker, ready to load.

Supplier: Thermo Scientific Chemicals

Acts as a protein precipitant used to purify proteins. It is used as chelating agent and as a scavenger for aldehydes and oxides. Also used in detergents, paper industry, dyes, printing inks and in water treatment.

Supplier: Thermo Scientific Chemicals

Non-ionic detergent for membrane proteins

Supplier: MP Biomedicals

Glycine is a non-essential amino acid. Acts as an inhibitory neurotransmitter, particularly in brain stem and medulla. Shown to block NMDA-stimulated dopamine release in fetal mesencephalic cell cultures. Also reported to increase NMDA-receptor mediated synaptic potential in cortical neurons. It does not have a chiral center, so only amino acid with no asymmetric carbon. Therefore, it does not have a D- or L- isomer designation. This material is synthetic. It is the smallest of the 20 natural amino acids.

Supplier: VWR

At a concentration of 0.5μg/ml, ethidium bromide is useful in agarose or acrylamide gels for band detection. It is used to detect as little as 10ng of nucleic acid. At higher concentrations (0.5–1.0μg/ml), it may be used to facilitate purification of DNA in a cesium chloride gradient.

Supplier: Rockland Immunochemical

Lyophilized Bovine Serum Albumin - Fraction V, pH 7.0.

Supplier: MP Biomedicals

Soluble in water (1 g/8.3 mL at 25 °C; 1 g/4 mL boiling water) or glycerol (1 g/75 mL); insoluble in ethanol; the solubility in water is decreased in the presence of KCl or (NH4)2SO4 and is practically insoluble in a saturated solution of the latter.

Supplier: Promega Corporation

36-2,645bp; 15 bands. Can be stored at room temperature. Contains dye marker, ready to load. Combination of three separate digests of pGEM(R)-3 Vector.

Supplier: Thermo Scientific Chemicals

99% 25G

Supplier: Cytiva

Ab SpinTrap columns are prepacked, single-use spin columns for rapid purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. Designed for small-scale purification of multiple samples in parallel.

Supplier: Invitrogen

The Pierce Silver Stain Kit is a rapid, ultra-sensitive, and versatile silver stain system for protein detection in polyacrylamide gels, yielding consistent and reliable results. It is a metallic silver (Ag) protein stain that yields a remarkably clear and uniform gel background. In standard mini gels, proteins are detectable at greater than 0.25 ng per band or spot. The protocol has been optimized for flexibility by allowing short or overnight gel fixation and staining steps without affecting staining performance (sensitivity or clarity). A short (one-minute) sensitization step, performed after gel fixation, yields results that are free of the characteristically dark or blotchy backgrounds often seen with homemade or other commercially available silver stains. This feature is beneficial when performing densitometric analysis of silver stained gels.

Supplier: MP Biomedicals

SDS is an anionic detergent and wetting agent that is effective in both acid and alkaline solutions.
Used to solubilize and denature proteins for denaturing-PAGE. Most proteins bind SDS in a ratio of 1.4 g SDS per gram of protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on their size.

Supplier: Prosci

This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Supplier: IBI Scientific

3:1 Super-Sieve Agarose is designed to have a larger gel network than IBI Ultra Sieve Agarose and is recommended for use in the separation of DNA fragments smaller than 1500bp

Supplier: Promega Corporation

100-1,500bp in 100bp increments up to 1kb with an additional band at 1.5kb; 11 bands. Can be stored at room temperature. Contains dye marker, ready to load.

Supplier: Quantabio

The qScript™ XLT One-Step RT-PCR Kit is a convenient and highly sensitive system for amplification of RNA templates up to 2 kb

Supplier: Promega Corporation

Proteinase K, produced by the fungus Tritirachium album Limber, is a serine protease that exhibits broad cleavage activity. Proteinase K (PK) Solution is supplied at a concentration of 20mg/ml in 10mM Tris-HCl (pH 7.5), 1mM calcium chloride, 50% glycerol.

Supplier: MP Biomedicals

Polysorbate 20 is nonionic surfactant derived from sorbitan ester.

Supplier: MP Biomedicals

Polysorbate 20 is nonionic surfactant derived from sorbitan ester.

Supplier: AVIDITY SCIENCE

Two-In-One system provides DI or RO water to a storage tank, and capable of dispensing Type 1 and Type 2 or Type 3 water from dispense guns (depending on model variation).

Supplier: MP Biomedicals

Reagent for immunohistology and immunoblotting.

Supplier: Invitrogen

Pierce™ Glycoprotein Carbohydrate Estimation Kit enables the amount of protein glycosylation to be measured as a percentage of total purified protein mass.

Supplier: AVIDITY SCIENCE

Two-In-One system provides DI water to a storage tank and ultrapure water through dispensing gun.

Supplier: AVIDITY SCIENCE

Provides laboratory grade, Type 2 water in two convenient production rates of 20 or 40 liters per hour.

Supplier: MP Biomedicals

DL-Dithiothreitol is a Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.

Supplier: Invitrogen

LightShift® Chemiluminescent EMSA Kit is robust and sensitive system for performing electrophoretic mobility shift assays (EMSAs) to identify and characterize protein:DNA binding interactions.

Supplier: MP Biomedicals

Sodium chloride is a commonly used chemical which is found widely in nature. It is considered to be an essential nutrient. Excess amounts of sodium chloride can destroy electrolyte balance and cause death in most animals, including humans.
Sodium chloride is used in a wide variety of biochemical applications, including intravenous fluids (normal saline is 0.9% w/v in water 10), density gradients 3,6, a diluent to increase ionic strength in buffers or culture media and in salt-out procedures in the isolation of DNA. It has also been used in high concentrations for preservation of foods since bacteria cannot grow in high salt conditions. A salt-ice mixture in the ratio of approximately 33 g sodium chloride to 100 g ice (at -1°C) will drop in temperature to as low as -21°C, depending on the rate of stirring and the size of the ice chunks.

Supplier: 0000027876

This is a AMY1A recombinant protein (prokaryotic), Human is sequencing from Ala15~Leu511 with 95 - 100% purity. Lyophilized from PBS, pH 7.4, containing 0.01% SKL, 5% Trehalose with 0.2 mg/ml.

Supplier: IBI Scientific

IBI gBAC Mini DNA Bacteria Kit is optimized for genomic and viral DNA purification from Gram (-) negative and Gram (+) positive bacterial cells

Supplier: Promega Corporation

New formulation of Taq DNA Polymerase with enhanced performance over standard Taq DNA Polymerase. Available in two all-in-one master mixes: green and colorless. The green master mix allows direct gel analysis after amplification.