36 Results for: "EMPIRICAL BIOSCIENCE"

Supplier: EMPIRICAL BIOSCIENCE

QuantiTASE™ ULTRA OneStep RT-qPCR Universal Kits

Supplier: EMPIRICAL BIOSCIENCE

ROX Reference Dye

Supplier: EMPIRICAL BIOSCIENCE

LwCas13 is an RNA-guided ribonuclease that is naturally expressed by Leptotrichia wadei.

Supplier: EMPIRICAL BIOSCIENCE

This enzyme is a uracil N-glycosylase (UNG) obtained from the bacterium Esherichia coli. UNG enzymes specifically hydrolyze the N-glycosidic bond of uracil residues in ss/dsDNA molecules leaving an abasic site in the strand but will not react with RNA. The optimal temperature range for E. coli UNG activity is from 30 to 40 °C. The enzyme is irreversibly inactivated after ten minutes of incubation at temperatures ≥95 °C making it suited for carryover prevention in standard PCR and qPCR applications. It is active in most standard reaction buffers used for derivatives of Taq and Pfu DNA polymerases.

Supplier: EMPIRICAL BIOSCIENCE

Chemically modified Taq DNA polymerase designed for reducing non-specific DNA amplification due to primer-dimer formation. FlashTaq™ is completely suppressed prior to activation and regains full enzymatic activity in only 2 min at 95 °C. Taq DNA Polymerase gene is isolated from Thermus aquaticus YT1 and expressed in E. coli. FlashTaq™ HotStart DNA Polymerase is supplied in a storage buffer containing: HEPES, KCl, EDTA, DTT, Glycerol, and other stabilizers.

Supplier: EMPIRICAL BIOSCIENCE

RTScript™ cDNA Transcriptase is a Modified version of M-MLV Reverse Transcriptase with RNase H activity deactivated and increased thermal stability. In combination with optimized buffers and reagents, Empirical's RTScript™ enzyme synthesizes complementary DNA from RNA initiated from RNA or DNA Primers.

Supplier: EMPIRICAL BIOSCIENCE

Supplied at a 2X concentration and contains dATP, dTTP, dCTP, dGTP, and other buffer components and salts including Tris-HCl, MgCl₂, and KCl.

Supplier: EMPIRICAL BIOSCIENCE

Inhibitor tolerant reverse transcription PCR/qPCR, reverse transcription PCR/qPCR.

Supplier: EMPIRICAL BIOSCIENCE

UltraPure deoxynucleotide solution set is suitable for molecular biology applications including PCR, qPCR, RT-PCR, DNA labeling, DNA sequencing and primer extension.

Supplier: EMPIRICAL BIOSCIENCE

Deoxynucleotide solution mix: A 10 mM equimolar solution of ultrapure dATP, dCTP, dTTP, and dGTP supplied as clear aqueous solution.

Supplier: EMPIRICAL BIOSCIENCE

Cyclenase is a thermostable DNA polymerase that lacks exonuclease activity in both the 3 - 5' and 5 - 3' directions. Cyclenase efficiently incorporates dideoxynucleotide triphosphates (ddNTPs), making it useful for manual and automated sequencing applications. In addition to its DNA polymerase, Cyclenase includes a thermophilic acidic pyrophosphatase (TAPP) that converts the pyrophosphate product of nucleotide polymerization into inorganic orthophosphate.

Supplier: EMPIRICAL BIOSCIENCE

An enhanced performance MasterMix containing UDG Enzyme and dUTP to minimize the effects of carry over contamination. The MasterMix is a 4X ready-to-use MasterMix for quantitative real time evaluation of DNA using dye- or fluorescent probe-based detection.

Supplier: EMPIRICAL BIOSCIENCE

Thermo stable enzyme gene isolated from Thermus aquaticus YT1 and expressed in E. coli.

Supplier: EMPIRICAL BIOSCIENCE

This is a ready-to-use master mix for one-step, reverse-transcription quantitative PCR (RT-qPCR) detection of specific RNA sequences using dye or fluorescent probe-based detection.

Supplier: EMPIRICAL BIOSCIENCE

T4 DNA Polymerase

Supplier: EMPIRICAL BIOSCIENCE

Taq 2X MasterMix supplied as a 2X reaction buffer with dCTP, dGTP, dATP, dTTP, MgCl₂, and Taq DNA polymerase. Taq DNA polymerase gene was isolated from thermus aquaticus YT1 and expressed in E. coli. Empirical's taq 2X MasterMix is easy to use, versatile, and provides excellent amplification for many PCR based applications. Just add template and primers with the MasterMix and the reaction is ready to go.

Supplier: EMPIRICAL BIOSCIENCE

Magnesium chloride 25 mM

Supplier: EMPIRICAL BIOSCIENCE

LbCas12a is an RNA-guided endonuclease that is naturally expressed by Lachnospiraceae bacterium. The recombinant enzyme is engineered as a fusion protein with a eukaryotic nuclear localization signal (NLS) at the C-terminus to promote import of LbCas12a/gRNA ribonucleoprotein (RNP) complexes into the mammalian cell nucleus for genome engineering purposes.

Supplier: EMPIRICAL BIOSCIENCE

qPCR Probe MasterMix: Supplied as a 2X ready-to-use MasterMix for quantitative real time evaluation of DNA using fluorescent probe-based detection.

Supplier: EMPIRICAL BIOSCIENCE

FlashTaq™ HotStart 2X MeanGreen™ MasterMix contains FlashTaq™ HotStart DNA polymerase; a chemically modified HotStart Taq DNA polymerase.

Supplier: EMPIRICAL BIOSCIENCE

2X InhibiTaq™ Multiplex PLUS HotStart MasterMix.

Supplier: EMPIRICAL BIOSCIENCE

The qPCR MasterMix is supplied as a 2X product and is a ready to use HotStart qPCR MasterMix with EvaGreen® dye, FlashTaq DNA polymerase, dCTP, dGTP, dATP, dTTP, and MgCl2. EvaGreen® is a dsDNA-binding dye. EvaGreen® possesses similar absorption and emission spectra as SYBR® Green I.

Supplier: EMPIRICAL BIOSCIENCE

This enzyme is a thermolabile uracil N-glycosylase (UNG) obtained from Atlantic cod (Gadus morhua). UNG enzymes specifically hydrolyze the N-glycosidic bond of uracil residues in ss/dsDNA molecules leaving an abasic site in the strand but will not react with RNA.

Supplier: EMPIRICAL BIOSCIENCE

Proteinase K is a broad-spectrum endopeptidase derived from the fungus Tritirachium album. The native enzyme has a size of 26 to 28 kDa and preferentially cleaves peptide bonds adjacent to aliphatic and aromatic amino acid residues of both native and denatured proteins.

Supplier: EMPIRICAL BIOSCIENCE

Cas9 is an RNA-guided endonuclease that is naturally expressed by Streptococcus pyogenes. Catalytically active ribonucleoprotein (RNP) complexes are produced by incubating Cas9 with a sequence specific CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA), which together activate the endonuclease and guide it to the ~20-nucleotide protospacer sequence in target dsDNA. Alternatively, Cas9 can be complexed with a synthetic guide RNA (sgRNA) that encodes both the crRNA and tracrRNA in a single RNA strand.

Supplier: EMPIRICAL BIOSCIENCE

Reverse transcriptase enzymes are RNA-directed DNA polymerases that catalyzes first strand cDNA synthesis from oligonucleotide primed RNA templates.

Supplier: EMPIRICAL BIOSCIENCE

2X InhibiTaq™ hot start qPCR master mix is a 2X ready-to-use master mix for quantitative real time evaluation of DNA using dye- or fluorescent probe-based detection. InhibiTaq™ hot start qPCR master mix contains a chemically modified taq DNA polymerase that remains completely inactive at room temperature. The enzyme becomes activated after only 2 minutes at 95 °C.

Supplier: EMPIRICAL BIOSCIENCE

FlashTaq™ HotStart 2X MasterMix contains FlashTaq HotStart DNA Polymerase, a chemically modified HotStart Taq DNA polymerase.

Supplier: EMPIRICAL BIOSCIENCE

Empirical's ActiTemp-50 RTScript™ Reverse Transcriptase is a modified version of M-MLV Reverse Transcriptase with RNase H activity deactivated and increased thermal stability. The enzyme is Warm-Start modified with a proprietary RNA Aptamer.

Supplier: EMPIRICAL BIOSCIENCE

BST Prime is a recombinantly produced Bacillus stearothermophilus DNA polymerase I, large fragment (BST DNA Polymerase, large fragment).