166 Results for: "Denatured alcohol"

Supplier: Steris

Disinfectant for microbial control.

Supplier: Decon Labs

140 proof pure (undenatured) ethanol (70%) USP. Used for research, healthcare, industrial, and production applications as a cleaner, solvent, and reagent.

Supplier: Decon Labs

Pure (undenatured) anhydrous (200 proof/100%) ethyl alcohol meets USP and Multicompendial specifications used as a cleaner, solvent, or reagent.

Supplier: MP Biomedicals

Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: Wards

Replacement set of 100% round cotton wicks for alcohol lamps.

Supplier: Lab-Aids

With this kit students study the principles of conductors and insulators; study and develop the principles of electrolytes and non-electrolytes using acids, bases, salts, alcohols, and hydrocarbons; test a variety of common materials for their conductivity characteristics; and learn to differentiate between strong, weak, and non-conductors of electricity.

Supplier: Veltek Associates

STER-AHOL® Wipes may be used as a sanitizer in classified manufacturing and testing environments.

Supplier: Decon Labs

Decon’s 200 proof ethanol (100% ethanol) is a Molecular Biology Grade, pure ethanol product made to USP specifications and is tested for DNAase / RNAase activity and its ability to precipitate DNA from solution. Molecular Biology Grade Ethanol can be used in absolute form or diluted for use in most applications in Molecular Biology Laboratories.

Ordering Information: Pure Ethanol products are taxed according to the government schedule of excise taxes detailed in 27 CFR Chapter 1 section 27.41. This excise tax is included in the cost of this product. There is no need for Federal Permits.

Supplier: MP Biomedicals

Magnesium chloride hexahydrate (MgCl2·6H2O) is an inorganic salt that can be generated from the acid leach solution of magnesite tailings. It is a potential phase change material (PCM) for storing thermal energy in solar cookers. The result of altering poly (vinyl alcohol)(PVA) films with MgCl2·6H2O has been investigated. The ability of MgCl2·6H2O to reduce the flammability of cotton fabrics has been studied.

Supplier: MP Biomedicals

β-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been commonly used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

Supplier: MP Biomedicals

Storage: Room Temperature
2-Mercaptoethanol is a hybrid of ethylene glycol and 1,2-ethanedithiol. It is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. It is widely used because the hydroxyl group confers solubility in water and lowers the volatility. Due to its diminished vapor pressure, its odour, while unpleasant, is less objectionable than related thiols.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also it reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

Supplier: United Scientific Supplies

Precision apparatus uses sound waves to clearly illustrate the often poorly understood phase and group velocity concepts

Supplier: MP Biomedicals

2-Merceptoethanol is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. 2-Merceptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis. 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

Supplier: IBI Scientific

Kit is designed to recover or concentrate DNA fragments (50bp–10kb) from agarose gel, PCR, or other enzymatic reactions

Supplier: IBI Scientific

The Large DNA Fragment Extraction Kits are designed to recover large DNA fragments (>8Kb) from an agarose gel

Supplier: IBI Scientific

The IBI high throughput 96-Well PCR Clean-Up Kit is designed to recover or concentrate DNA fragments from PCR or other enzymatic reactions using an efficient 96-well binding plate system.