"Cube Biotech"

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Human Recombinant MSP1E3D1 (from E. coli)

Supplier: Cube Biotech

Membrane scaffold protein, human origin.

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AASTY Screening Kit

Supplier: Cube Biotech

Each membrane protein has its native lipid environment. Therefore, each lipid environment is unique to each membrane protein. Moreover, it is this lipid environment that synthetic polymers like AASTYs interact with. Therefore, one needs a broader selection of polymers since there is not one AASTY that fits each phospholipid composition. To address this question, AASTYs come in three different ratios of acrylic acid to styrene. The ratios differ in hydrophilic property of AASTY, which results in different affinities to varying membrane lipid compositions. The second feature to screen for when it comes to AASTY is the size of the resulting nanodiscs. One of AASTY's most excellent features, compared to SMA or DIBMA, is that its nanodiscs are pretty uniform in size. Therefore, the correct fitting nanodisc size must be determined before.

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HighSpec Rho Starter Set 1: HighSpec Rho1D4 Agarose + Rho1D4 Peptide

Supplier: Cube Biotech

HighSpec Rho1D4 agarose resin is a suspension of 40 µm agarose beads, coupled to an anti-Rho1D4 antibody. It is meant for protein purification assays of the highest possible purity, exploiting the specificity of an antibody to its antigen.

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PureCube Co-NTA MagBeads

Supplier: Cube Biotech

PureCube Co-NTA magnetic beads/MagBeads were developed by Cube Biotech for His-tag protein purification.

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PureCube Ti-NTA MagBeads

Supplier: Cube Biotech

PureCube Ti-NTA MagBeads/magnetic beads were developed for the enrichment of phosphorylated proteins. They can also be used for the purification of His-tagged proteins as a secondary function.

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Nanodisc Msp1D1-His DMPG

Supplier: Cube Biotech

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

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SMALP BZ35 Membrane protein solubilization & stabilization

Supplier: Cube Biotech

The SMALP BZ series was developed in collaboration with Prof. Bert Klumperman from Nanosene, a spinout company of Stellenbosch University in South Africa. The goal was to create SMALPs that are synthesized via RAFT polymerization, achieving a better defined polymer size and a low dispersity (narrow chain length distribution). This is a significant improvement over previous SMALPs. The SMALP BZs are thus more efficient in dissolving the cell membranes and increasing protein yield.
After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

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Sulfo-SMA Membrane protein solubilization & stabilization

Supplier: Cube Biotech

Sulfo-SMA is an electroneutral modification of existing SMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-SMA belongs to a new generation of SMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10.
The special physicochemical properties of Sulfo-SMAs make them ideal for cryo-TEM and other downstream applications.
After successful membrane protein solubilization, the protein can be purified using affinity chromatography. For membrane protein purification, we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.
Good publications to find details about Sulfo-SMA and Sulfo-DIBMA are:
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)

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N-Octyl-β-D-glucopyranoside ≥99.5%, Crystallization grade

Supplier: Cube Biotech

Octylglucopyranoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer glucosides with chain lengths from C-8 to C-10, as well as octylthioglucoside.