"Biotium"

Supplier: Biotium

CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 alpha chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cell express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4 /CD8-. Anaplastic large cell lymphoma is usually CD4 and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®647 is a far-red fluorescent dye (Ex/Em 650/665 nm) with excellent brightness. It also is compatible with super-resolution imaging by STORM.

Supplier: Biotium

IL-6 (Rat), Monoclonal antibody, Clone: MP5-20F3, Host: Rat, Species reactivity: Mouse, Isotype: IgG2a, kappa, Conjugate: biotin, Immunogen: Recombinant mouse IL-6 protein, Application: Immunofluorescence, Neutralization, Flow cytometry, Size: 500uL

Supplier: Biotium

This MAb reacts with MUC1. The dominant epitope of this MAb has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®405S is a blue fluorescent dye (Ex/Em 404/431 nm) with superior brightness compared to other blue dyes; it is also compatible with super-resolution imaging by SIM. Note: Conjugates of blue fluorescent dyes are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Supplier: Biotium

This antibody recognizes a single protein of 33-34 kDa, identified as the prostate specific antigen (PSA). This MAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®594 is a deep red fluorescent dye (Ex/Em 593/614 nm). It yields the brightest conjugates among spectrally similar dyes, and has excellent photostability.

Supplier: Biotium

Anti-IgG Goat Polyclonal Antibody (CF™ 350)

Supplier: Biotium

Von Willebrand Factor, Monoclonal antibody, Clone: VWF635, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, BSA-free, Immunogen: Recombinant vWF fragment spanning aa 845-949, Synonyms: Coagulation Factor VIII, Application: IF, Size: 50 uL

Supplier: Biotium

Anti-IgY Rabbit Antibody (CF™ 633)

Supplier: Biotium

Eosinophil Peroxidase Monoclonal antibody, Clone: EPO104, Host: Mouse, Species reactivity: Human, Isotype: IgG1, K, Conjugate: CF568, Immunogen: Human eosinophils, hypereosinophilic syndrome, Synonyms: EPER, Application: IF, Size: 100uL

Supplier: Biotium

CD1A Monoclonal antibody, Clone: CB-T6, Host: Mouse, Species reactivity: Mouse, Human, Isotype: IgG1, kappa, Conjugate: CF568, Immunogen: Human CD1a, Synonyms: Cortical thymocyte antigen CD1A, Application: Immunofluorescence, Flow cytometry, Size: 100uL

Supplier: Biotium

This antibody recognizes a protein of 30 kDa, identified as CD70. It is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T- and B-lymphocytes. It induces proliferation of co-stimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®405S is a blue fluorescent dye (Ex/Em 404/431 nm) with superior brightness compared to other blue dyes; it is also compatible with super-resolution imaging by SIM. Note: Conjugates of blue fluorescent dyes are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Supplier: Biotium

Anti-IgG Goat Polyclonal Antibody (CF™ 514)

Supplier: Biotium

IgM Immunoglobulin, Monoclonal antibody, Clone: DA4-4, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, Conjugate: CF405S, Immunogen: Heavy chain of human IgM, Synonyms: AGM1; IGHM, Application: IF, IHC (formalin), Flow, Size: 100uL

Supplier: Biotium

This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.

Supplier: Biotium

In Western blotting, this antibody detects an antigen of 125 kDa in human liver and 135 kDa in tumors of histiocytic origin. Comparative study of this MAb and a standard CD68 MAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This MAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This MAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®488A is a green fluorescent dye (Ex/Em 490/515 nm) with excellent brightness and photostability. The dye is minimally charged for less non-specific binding. CF®488A also is compatible with super-resolution imaging by TIRF.

Supplier: Biotium

A lectin labeled with our superior fluorescent CF® dyes is an effective and widely used cell surface stain for yeast, fungi, and mammalian cells.

Supplier: Biotium

HLA-DRB, Monoclonal antibody, Clone: LN-3 + HLA-DRB/1067, Host: Mouse, Species reactivity: Human, Monkey, Isotype: IgG's, Conjugate: CF405S, Immunogen: Activated human peripheral blood mononuclear cells (LN-3 & HLA-DRB/1067), Application: IF, IHC Western, Size: 100uL