"Biotium"

Supplier: Biotium

Anti-IgG Bovine Polyclonal Antibody (CF™ 594)

Supplier: Biotium

This antibody recognizes a protein of 30-33 kDa, which is identified as CD20 (Workshop V; Code CD20.12. Workshop IV; Code B17). It This antibody recognizes an extracellular domain of CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. The protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as calcium channel involved in B cell activation and cell cycle progression.

Supplier: Biotium

This antibody recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7 ) from colon carcinomas (keratin 7-).

Supplier: Biotium

PAb122 binds to the C-terminus (aa370-378) of both wild type and mutated p53. When microinjected into nuclei, PAb122 blocked re-entry into the S-phase of the cell cycle. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. p53 Binds to a DNA consensus sequence, the p53 response element, and it regulates normal cell growth cycle events by activating transcription of genes, involved either in progression through the cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. p53 Is the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation.

Supplier: Biotium

CD282 / TLR2 Monoclonal antibody, Clone: TLR2/221, Host: Mouse, Conjugate: CF647, Species reactivity: Human, Monkey, Dog, Immunogen: Recombinant human TLR2 protein, Isotype: IgG2a, kappa, Synonyms:CD282, Application: IF, IHC (formalin), FC, Size: 100uL

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HER-2 / CD340, Monoclonal antibody, Clone: HRB2/273, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, Conjugate: CF594, Immunogen: Recombinant intracellular domain of human HER-2 protein, Synonyms: p185, CD340, Verb b2, Size: 500uL

Supplier: Biotium

Mucin 3, Monoclonal antibody, Clone: MUC3/1154, Host: Mouse, Species reactivity: Human, Isotype: IgG2b, kappa, Conjugate: CF488A, Immunogen: synthetic peptide of 35 amino acids, SIB35, Synonyms: Intestinal mucin-3A; intestinal mucin-like, Application: IF, Size: 500uL

Supplier: Biotium

This antibody reacts with five invariable CD3 chains designated CD3y, CD3d, CD3e, CD3f, and CD3n with molecular weight ranging from 16-28 kDa. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly intracytoplasmic. This MAb is particularly useful for induction of T cell activation in vitro and in vivo due to its unusual IgG3 isotype.

Supplier: Biotium

This antibody recognizes a protein of 205 kDa-220 kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4 T-cells, 50% of peripheral CD8 T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4 or CD8 T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO ve) from B cell lymphomas (CD45RA ve).

CF® dyes are Biotium's next-generation fluorescent dyes. CF®640R is a far-red fluorescent dye (Ex/Em 642/662 nm) with excellent brightness, and the best photostabiity among spectrally-similar dyes.

Supplier: Biotium

CD63 Monoclonal antibody, Clone: NKI-C3, Host: Mouse, Species reactivity: Mouse, Human, Isotype: IgG1, kappa, Conjugate: CF405S, Immunogen: Smooth plasma membrane fraction of MeWo cells, Application: IF, Immunohistology (formalin), Western, FC, Size: 500 uL

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Complement C4d Monoclonal antibody, Clone: C4D205, Host: Mouse, Species reactivity: Human, Isotype: IgG1, Conjugate: CF640R, Immunogen: Recombinant human Complement 4d proteinUnigene 534847 & 720022, Application: Immunofluorescence, Size: 100uL

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P21 / WAF1, Monoclonal antibody, Clone: CIP1/823 + DCS-60.2, Host: Mouse, Species reactivity: Human, Isotype: IgG's, Conjugate: purified, Immunogen: Recombinant full-length human CDKN1A protein (CIP1/823); Human recombinant p21 protein (DCS-60.2), Application: IF, Size: 100uL

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Cytokeratin 18 Monoclonal antibody, Clone: KRT18/834, Host: Mouse, Species reactivity: Human, Isotype: IgG's, Conjugate: CF594, Immunogen: Recom full-length human KRT18 protein, Synonyms: K18, Application: IF, IHC, FC, Size: 500 uL

Supplier: Biotium

Ep-CAM / CD326 Monoclonal antibody, Clone: EGP40/1120, Host: Mouse, Conjugate: CF647, Species reactivity: Human, Immunogen: Recombinant full-length human EpCAM protein, Isotype: IgG1, kappa, Application: IF, FC, IHC, WB, Size: 100uL

Supplier: Biotium

Anti-IgG2 beta Goat Polyclonal Antibody (CF™ 488A)

Supplier: Biotium

CD284 / TLR4 Monoclonal antibody, Clone: TLR4/230, Host: Mouse, Species reactivity: Guinea Pig, Monkey, Pig, Dog, Rat, Human, Isotype: IgG2a, K, Conjugate: CF568, Immunogen: Recom human TLR4, Synonyms: ARMD10, CD284, hToll, TLR4, Application: IF, Size: 500uL