"Biotium"

Supplier: Biotium

Pmel17 / gp100 / SILV, Monoclonal antibody, Clone: NKI-beteb, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, Conjugate: CF647, Immunogen: Extract of pigmented melanoma metastases from lymph nodes, Application: Immunofluorescence, Flow, Size: 100uL

Supplier: Biotium

CD45 / LCA Monoclonal antibody, Clone: 2B11 + PD7/26, Host: Mouse, Species reactivity: Human, Dog, Isotype: IgG's, Conjugate: CF770, Immunogen: Isolated neoplastic cells from T cell lymphoma, Synonyms: B220, CD45R, Application: IF, Size: 500 uL

Supplier: Biotium

IDH1, Monoclonal antibody, Clone: IDH1/1152, Host: Mouse, Species reactivity: Human, Isotype: IgG1 and IgG2a, kappa, Conjugate: CF594, Immunogen: Recombinant fragment (119 Amino acid residues around aa 280-420) of human IDH1 protein, Application: IF, IHC Western, Size: 100uL

Supplier: Biotium

This antibody recognizes a protein of 36 kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®640R is a far-red fluorescent dye (Ex/Em 642/662 nm) with excellent brightness, and the best photostabiity among spectrally-similar dyes.

Supplier: Biotium

CD99 Monoclonal antibody, Clone: MIC2/877, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, Conjugate: CF594, Immunogen: Recom full-length human MIC2 protein, Synonyms: 12E7, E2 antigen, MIC 2X, MIC 2Y, MIC2, Application: IF, IHC, FC, Size: 500 uL

Supplier: Biotium

This antibody recognizes a protein of 57 kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts and stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts and stromal cells stain. The histological differentiation of complete mole, partial mole, and hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease and choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei and stromal cells are labeled with this antibody. Similar findings apply to PHM and hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities and serve as an internal control.

Supplier: Biotium

This antibody recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®568 is a red fluorescent dye (Ex/Em 562/583 nm) with superior brightness and photostability. It also is compatible with super-resolution imaging by STORM and TIRF.

Supplier: Biotium

This antibody recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®488A is a green fluorescent dye (Ex/Em 490/515 nm) with excellent brightness and photostability. The dye is minimally charged for less non-specific binding. CF®488A also is compatible with super-resolution imaging by TIRF.

Supplier: Biotium

Blue Fluorescent Cf405M (Ex/Em 408/452 Nm) Is Spectrally Similar To Pacific Blue, With Superior Photostability; Antibody Has Minimal Cross-Reactivity To Bovine, Chicken, Goat, Horse, Human, Mouse, Rabbit, Sheep, And Syrian Hamster Serum Proteins

Supplier: Biotium

Cytokeratin 8/18 Monoclonal antibody, Clone: C-43 + DC10, Host: Mouse, Species reactivity: Human, Isotype: IgG1, K, Conjugate: CF568, Immunogen: Cytoskeletal preparation from HeLa epitheliod carcinoma cells and PMC-42 breast carcinoma cells, Size: 500 uL

Supplier: Biotium

LipidSpot™ stains are fluorescent dyes that rapidly stain lipid droplets in live or fixed cells, with no wash step and minimal background.

Supplier: Biotium

Anti-IgG Goat Polyclonal Antibody (CF™ 633)

Supplier: Biotium

P21 / WAF1, Monoclonal antibody, Clone: CIP1/823, Host: Mouse, Species reactivity: Human, Isotype: IgG's, Conjugate: purified, Immunogen: Recombinant full-length human CDKN1A protein (CIP1/823); Human recombinant p21 protein (DCS-60.2), Application: IF, IHC, Size: 500uL

Supplier: Biotium

CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkins disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

Supplier: Biotium

Involucrin, Monoclonal antibody, Clone: IVRN/827, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, Conjugate: CF647, Immunogen: Purified involucrin from human keratinocytes, Synonyms: IVL, Application: Immunofluorescence, Flow cytometry, Size: 500uL

Supplier: Biotium

This antibody recognizes a protein of ~55 kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.