D-(+)-Biotin ≥97.5%, white crystalline powder cell culture reagent
Supplier: MP Biomedicals
Storage: +4 °C
D-Biotin is a growth factor present in small amounts in every living cell. It is involved in naturally occurring carboxylation reactions. It occurs mainly bound to proteins or polypeptides. It is more abundant in the liver, kidney, pancreas, yeast and milk. Biotin levels are higher in cancerous tumors than in normal tissues. It is inactivated by binding to avidin.
D-Biotin may be used to elute proteins from avidin/streptavidin resins. It is widely used for dietary supplements and fortified foods. It is also used for tablets and hard-shell capsule preparation due to its pharmaceutical properties.
Essential vitamin that is important for amino acid and energy metabolism, and fatty acid synthesis. It is a prosthetic group in four mammalian carboxylase families and facilitates the binding and transfer of carbon dioxide.
Soluble in water (22 mg/100 mL), ethanol (80 mg/100 mL), more soluble in hot water and in dilute alkalies; insoluble in other common organic solvents. Soluble in 2 M Ammonium hydroxide (50 mg/mL - clear, colorless solution), dimethylformamide (1.7 mg/mL).
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Corning® MEM (Minimum Essential Medium) Alpha Medium, Corning
Supplier: Corning
Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells.
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6-Benzylaminopurine, Powder
Supplier: MP Biomedicals
6-Benzylaminopurine (BAP) is a plant cell division factor (a plant growth factor).
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RPMI 1640 Medium, MP Biomedical, LLC.
Supplier: MP Biomedicals
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Since, it has been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes.
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Sodium-L(+)-glutamate monohydrate ≥99%, white powder cell culture reagent
Supplier: MP Biomedicals
L-Glutamic Acid is a non-essential amino acid for human development; referred to as an excitatory amino acid (EAA) due to its role in neurotransmission. Acts as a neurotransmitter at fast synapses; agonist at kainate, NMDA and quisqualate receptors. L-Glutamic acid is one of the two amino acids that contains a carboxylic acid group in its side chains.
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McCoy’s 5A Medium with L-Glutaminer, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
McCoy’s 5A Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. McCoy’s 5A Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.