Hygromycin B ≥90%

An aminoglycoside antibiotic produced by Streptomyces hygroscopicus that is routinely used as a selection agent for cells that contain hph or hyg Hygromycin resistant gene. Hygromycin B is thought to inhibit protein synthesis by strengthening the interaction of peptidyl-tRNA in the ribosomal acceptor ('A') site thereby preventing translocation of by EF-2 (elongation Factor-2). Hygromycin B is effective on most bacteria, fungi and higher eukaryotes.

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