pH Gradient Buffers

Deliver robust, reproducible gradients with the Thermo Scientific pH buffer platform, which is applicable to a wide range of MAbs and ready to use with existing LC columns and systems, without the need to formulate mobile phases.

• Proprietary buffer formulations enable fast, robust and reproducible pH gradients that are simple to optimise and easily automated
• Ready to use with existing LC columns and systems, without the need for time-consuming mobile phase adjustments
• Applicable to the majority of MAbs

The pH gradient platform saves time in method development and facilitates method transfer to QA/QC for a wide range of MAb charge variants through a generic LC-based approach to MAb characterization.

All that is required to generate a pH gradient is a 1:10 dilution of the pH buffer concentrates. A linear pH gradient from pH 5.6 to 10.2 can simply be delivered by running a pump gradient from 100% eluent A to 100% eluent B.