SuperBlock™ (PBS) Blocking Buffer, Thermo Scientific

SuperBlock™ Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.

• Blocking time—block membranes in 5 to 10 minutes and ELISA plates in 2 minutes
• Applications—ELISA, western blotting, IHC; biotin-free for use with streptavidin system
• Stability—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin, or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilising them for drying and storage for later use.