The NEBNext library quant kit components have been optimized to deliver significant improvements to qPCR-based library quantitation for Illumina sequencing.

• Be confident in your quant values, with more accurate and reproducible results
• Quantitate more libraries per kit, as only four standards are required
• Simplify your reaction setup with fewer pipetting steps and a single extension time for all libraries
• Use with all your libraries, regardless of insert size, GC content and preparation method

The kit contains primers which target the P5 and P7 Illumina adaptor sequences, and a set of four high-quality, pre-diluted DNA standards to enable reliable quantitation of diluted DNA libraries between 150 to 1000 bp.