NEBNext® LV Unique Dual Index Primers Set 2A includes 24 unique pairs of i5 and i7 index primers optimized for low-volume (LV) NEBNext workflows, including NEBNext EM-seq™ and E5hmC-seq™, with Illumina® sequencing.

• Optimized for the low-volume (LV) NEBNext library prep workflows

• Addresses the 'barcode hopping' issue seen with some Illumina sequencing instruments
• Increases sample identification specificity with unique dual index primer pairs

The primers enable incorporation of unique index pairs during library amplification and have been designed to minimize index hopping. The index sequences from the NEBNext LV Unique Dual Index Primers Set 2A  are compatible with and can be combined with the other NEBNext LV Unique Dual Index Primers sets to multiplex up to 120 reactions.

Note: NEBNext LV Unique Dual Index Primers Set 2A and Set 2B (NEB # E3392) are 24-index subsets of Set 2 (NEB #E3402); therefore, we do not recommend combining Set 2A and/or 2B with Set 2.