NEBNext Enzymatic Methyl-seq (EM-seq) is a high-performance enzyme-based alternative to bisulfite conversion for the identification of 5 mC and 5 hmC.

• Superior sensitivity of detection of 5mC and 5hmC

• 0.1 to 200 ng input range
• Detection of more CpGs with fewer sequencing reads
• Even GC coverage
• Index primers supplied separately

Unlike bisulfite conversion, this highly efficient method minimizes DNA damage, resulting in superior detection of methylated cytosines, with fewer sequencing reads. The new NEBNext Enzymatic Methyl-seq v2 Kit has a wider input range (as low as 100 pg) and a faster, more streamlined workflow than the original EM-seq kit.

The NEBNext Enzymatic Methyl-seq v2 Kit includes conversion reagents, library prep reagents and the EM-seq Adaptor. Multiple sets of the required index primers (NEBNext LV Unique Dual Index Primers) are available separately, enabling greater flexibility in multiplexing.