Human Recombinant CXCL10 (IP-10) (from E. coli)

Recombinant Human CXCL10 (IP-10) in lyopholized format with very low endotoxin levels.

Extremely low endotoxin levels (<0.01 EU per 1 μg) Functional activity tested and validated For use in various streptavidin applications: Flow cytometry, microscopy, and other imaging techniques

CXCL10 (IP-10) was originally identified as an IFN-gamma-inducible gene in endothelial, fibroblasts and monocytes cells. IP-10 is considered a member of the CXC chemokine subfamily from its protein sequence which includes the four conserved cysteine residues present in CXC chemokines. IP-10 signals through the CXCR3 receptor to selectively attract Th1 lymphocytes and monocytes. It also has angiostatic and mitogenic properties on vascular smooth muscle cells. A diverse population of cell types rapidly increases transcription of mRNA encoding IP-10, which suggests that gamma-induced protein may be a key mediator of the IFNG/IFN-gamma response.

: Larger sizes available, please request a custom quote.

: Shipped lyophilized at room temperature.

: For research use only, not for use in humans.

Conjugation:
Unconjugated
Protein Function:
Chemokine
Protein/Peptide Type:
Recombinant
Source:
E. coli
Species:
Human
Storage Conditions:
Avoid repeated freeze-thaw cycles 12 months from date of receipt, –20...–70 °C as supplied. 1 month, 2...8 °C under sterile conditions after reconstitution. 3 months, –20...–70 °C under sterile conditions after reconstitution.
Endotoxin Content:
<0.01 EU per 1 μg of the protein by the LAL method
Biological Activity:
EC50 = 6.3 nM determined by Migration Assay in cells expressing recombinant CXCR3
Gene ID:
Accession # P02778 (22-98)
Reconstitution Instructions:
Spin sample prior to reconstitution. Recommended concentration of 100 μg/ml in sterile water.
Endotoxin-free:
Y
Carrier-Free:
Y
Protease-free:
Y
Protein Synonyms:
C7|IFI10|INP10|IP-10|crg-2|mob-1|SCYB10|gIP-10
Protein/Peptide Name:
CXCL10 (IP-10)
Purity:
>97%
Molecular Weight:
8.65 kDa
Sequence:
VPLSRTVRCTCISISNQPVNPRSLEKLEIIPASQFCPRVEIIATMKKKGEKRCLNPESKAIKNLLKAVSKERSKRSP
Endotoxin Level:
Very low
Formulation:
Lyophilized pellet
Shipping Temperature:
RT
Tested Applications:
Migration assay