Human Recombinant CXCL8 (IL-8) (from E. coli), Biotinylated

Supplier: CHEMOTACTICS, INC MS

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Supplier: CHEMOTACTICS, INC MS

About this item

Functional biotin labeled human CXCL8 chemokine with very low endotoxin levels.

Extremely low endotoxin levels (<0.01 EU per 1 μg) Functional activity tested and validated For use in various streptavidin applications: Flow cytometry, microscopy, and other imaging techniques

Interleukin 8 (IL-8 )(CXCL8) is secreted primarily by macrophages and monocytes. It is one of the key mediators for inflammatory responses. IL-8 is a strong chemotractant for newtrophiles and monoccytes, and promotes activation of these target cells by binding to two cell surface receptors CXCR1 and CXCR2. It is also a strong angiogenic agent, and is considered to play a role in the pathogenesis of bronchiolitis.

Ordering information: Larger sizes available, please request a custom quote.

Delivery: Shipped lyophilized at room temperature.

Caution: For research use only, not for use in humans.

Specifications

Conjugation:
Biotin
Protein Function:
Chemokine
Protein/Peptide Type:
Recombinant
Source:
E. coli
Species:
Human
Storage Conditions:
Avoid repeated freeze-thaw cycles. 12 months from date of receipt, –20... –70 °C as supplied. ChemoTactics suggests using immediately after reconstitution. 1 month, –20...–70 °C under sterile conditions after reconstitution.
Endotoxin Content:
<0.01 EU per 1 μg of the protein by the LAL method
Biological Activity:
EC50 = 0.5 - 1.0 nM determined by Migration Assay in cells expressing recombinant CXCR1
Gene ID:
Accession # P10145 (28-99)
Reconstitution Instructions:
Spin sample prior to reconstitution. Recommended concentration of 100 μg/ml in sterile water.
Endotoxin-free:
Y
Carrier-Free:
Y
Protease-free:
Y
Protein Synonyms:
IL8|NAF|GCP1|LECT|LUCT|NAP1|GCP-1|LYNAP|MDNCF|MONAP|NAP-1|SCYB8
Protein/Peptide Name:
CXCL8 (IL-8)
Purity:
>97%
Molecular Weight:
10.80 kDa
Endotoxin Level:
Very low
Formulation:
Lyophilized pellet
Shipping Temperature:
RT
Tested Applications:
Migration assay