Human Recombinant CXCL12 (SDF-1a) (from E. coli), Biotinylated

Functional biotin labeled human CXCL12a chemokine with very low endotoxin levels.

Extremely low endotoxin levels (<0.01 EU per 1 μg) Functional activity tested and validated For use in various streptavidin applications: Flow cytometry, microscopy, and other imaging techniques

As a chemoattractant active on T-lymphocytes, monocytes, but not neutrophils, SDF-1α activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to another C-X-C chemokine receptor CXCR7, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1α (3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1α(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and Tlymphocytes through its receptors, CXCR4 and CXCR7, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF-1α/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of CXCR7 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.

: Larger sizes available, please request a custom quote.

: Shipped lyophilized at room temperature.

: For research use only, not for use in humans.

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Conjugation:
Biotin
Protein Function:
Chemokine
Protein/Peptide Type:
Recombinant
Source:
E. coli
Species:
Human
Storage Conditions:
Avoid repeated freeze-thaw cycles. 12 months from date of receipt, –20... –70 °C as supplied. ChemoTactics suggests using immediately after reconstitution. 1 month, –20...–70 °C under sterile conditions after reconstitution.
Endotoxin Content:
<0.01 EU per 1 μg of the protein by the LAL method
Biological Activity:
EC50 = 0.5 - 0.9 nM determined by Calcium Flux with human CXCR4 in U937 cells
Gene ID:
Accession # P48061-2 (22-89)
Reconstitution Instructions:
Spin sample prior to reconstitution. Recommended concentration of 100 μg/ml in sterile water.
Endotoxin-free:
Y
Carrier-Free:
Y
Protease-free:
Y
Protein Synonyms:
IRH|PBSF|SDF1|TLSF|TPAR1|SCYB12
Protein/Peptide Name:
CXCL12 (SDF-1a)
Purity:
>97%
Molecular Weight:
10.38 kDa
Endotoxin Level:
Very low
Formulation:
Lyophilized pellet
Shipping Temperature:
RT
Tested Applications:
Migration assay