Human Recombinant CCL19 (MIP-3beta) (from E. coli), Biotinylated

Supplier: CHEMOTACTICS, INC MS

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Supplier: CHEMOTACTICS, INC MS

About this item

Functional biotin labeled human CCL19 chemokine with very low endotoxin levels.

Extremely low endotoxin levels (<0.01 EU per 1 μg) Functional activity tested and validated For use in various streptavidin applications: Flow cytometry, microscopy, and other imaging techniques

Macrophage inflammatory protein-3-beta (MIP3β) (CCL19), also known as EBI1 ligand chemokine (ELC), directs chemotaxis of dendritic cells, and certain B- and T- lymphocytes, but not monocytes or granulocytes. It is constitutively expressed in thymus and lymph nodes and binds specifically to target cells expressing the receptor CCR7. Being a homeostatic chemokine, its primary physiological role is considered to be in the normal recirculation and homing of lymphocyte. However, MIP3β could also be proinfammatory, and is implicated in the post-HIV infection responses.

Ordering information: Larger sizes available, please request a custom quote.

Delivery: Shipped lyophilized at room temperature.

Caution: For research use only, not for use in humans.

Specifications

Conjugation:
Biotin
Protein Function:
Chemokine
Protein/Peptide Type:
Recombinant
Source:
E. coli
Species:
Human
Storage Conditions:
Avoid repeated freeze-thaw cycles. 12 months from date of receipt, –20... –70 °C as supplied. ChemoTactics suggests using immediately after reconstitution. 1 month, –20...–70 °C under sterile conditions after reconstitution.
Endotoxin Content:
<0.01 EU per 1 μg of the protein by the LAL method
Biological Activity:
EC50 = 15 - 30 nM determined by migration assay with cells expressing recombinant CCR7
Gene ID:
Accession Q99731 (22-98)
Reconstitution Instructions:
Spin sample prior to reconstitution. Recommended concentration of 100 μg/ml in sterile water.
Endotoxin-free:
Y
Carrier-Free:
Y
Protease-free:
Y
Protein Synonyms:
CKb11|ELC|MIP-3b|MIP3B|SCYA19
Protein/Peptide Name:
CCL19 (MIP-3beta)
Purity:
>97%
Molecular Weight:
11.22 kDa
Endotoxin Level:
Very low
Formulation:
Lyophilized pellet
Shipping Temperature:
RT
Tested Applications:
Migration assay