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- Application:
- Method / Format:Column (silica-based)
- Prep size:Giga
- Refine target molecule:Plasmid (endotoxin-free)
- Sample type:Bacteria
- Target molecule:Plasmid
- Description:ZymoPURE™ II Plasmid Gigaprep Kit
- Size:5 preps
- Environmentally Preferable:
- Sample Size:≤2.5 l
- Description:ZymoPURE™ II Plasmid Gigaprep Kit
- Yield:≤10 mg
- Cat. No.:77001-472
Part of the ZymoPURE plasmid kits collection, the ZymoPURE II™ plasmid midiprep kits provides the fastest and simplest method available to efficiently isolate up to 1.2 of transfection grade plasmid DNA from E. coli.
- Fastest: Simple 20 minute Midi/Maxi preps (50 min Giga)
- Highest Yield: 6× more plasmid
- Ultra-Pure: EndoZero™, vaccine grade and transfection ready
Utilizing a modified alkaline lysis in conjunction with a binding system, the ZymoPURE II midiprep kits can process up to 50 ml of culture in less than 18 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is rapidly bound onto a column with either a vacuum or a centrifuge instead of a slow gravity flow column. Additionally, there is no alcohol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 6 µg/µl), endotoxin-free and transfection-ready. As an added convenience, the ZymoPURE II™ plasmid midiprep kits contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.