Human Recombinant TNF-A (from E. coli)
Supplier: VWR International
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About this item
Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.
- High quality
- Low endotoxin
- Affordable
Caution: For research use only.
Specifications
- Conjugation:Unconjugated
- Protein Function:Cytokine
- Protein/Peptide Type:Recombinant
- Source:E. coli
- Species:Human
- Storage Conditions:–20 °C
- Endotoxin Content:Endotoxin LAL (EU/µg) ≤1
- Biological Activity:Bioactivity assay: Cytolysis of mouse L929 cells in the presence of Actinomycin D; ED50 ≤2 ng/ml; Bioactivity ≥5.0×10⁵ units/mg
- Gene ID:P01375
- Reconstitution Instructions:Sterile water at 0.1 mg/ml
- Endotoxin-free:N
- Carrier-Free:Y
- Protease-free:N
- Animal-Free:N
- Protein Synonyms:TNFSF2|Cachectin|DIF|Necrosin|Cytotoxin|Cachexin|TNF
- Protein/Peptide Name:TNF-A
- Purity:≥95%
- Molecular Weight:17.5 kDa
- Sequence:MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
- Endotoxin Level:Low
- Formulation:10 mM Sodium Phosphate, pH 7.5
- Nuclease-free:N
- Grade:RUO
- Shipping Temperature:RT
- Tested Applications:Bioassay
Specifications
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