Human Recombinant PDGF-BB (from E. coli)

Supplier: VWR International

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Supplier: VWR International

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About this item

PDGF-BB (Platelet-derived growth factor) comprises a family of homo or heterodimeric growth factors, including PDGF-AA, PDGF-BB, PDGF-CC, and PDGF-DD. Binds to two different transmembrane tyrosine kinase receptors (PDGFR-alpha and PDFGR-beta). Released from degranulating platelets and plays a role in each stage of wound healing.

High quality
Low endotoxin
Lot-to-lot consistency
Animal free Manufactured following GMP guidelines

Stimulates mitogenicity and chemotaxis of neutrophils, macrophages, fibroblasts, and smooth muscle cells to the wound site. Enhances proliferation of fibroblasts and their production of extracellular matrix. The biological activity of CTGrade GMP interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.

Host cell protein: ≤30 ng/ml; Host cell DNA: ≤0.01 ng/ml; Sterility testing: No growth; Mycoplasma: Negative

Caution: For research use or further manufacturing only.

Specifications

Conjugation:
Unconjugated
Protein Function:
Cytokine
Protein/Peptide Type:
Recombinant
Source:
E. coli
Species:
Human
Storage Conditions:
At or below –20 °C
Endotoxin Content:
Endotoxin LAL (EU/µg) ≤0.01
Biological Activity:
Bioactivity assay: Proliferation of NR6R-3T3 ; ED50 ≤5 ng/ml; Bioactivity ≥1.6 x10³ IU/µg
Gene ID:
P01127
Reconstitution Instructions:
Sterile water at 0.1-0.5 mg/ml
Endotoxin-free:
N
Carrier-Free:
Y
Protease-free:
N
Animal-Free:
Y
Protein Synonyms:
GDGF|ODGF
Protein/Peptide Name:
PDGF-BB
Purity:
≥98%
Molecular Weight:
12.4/24.9 kDa
Sequence:
MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT
Endotoxin Level:
Low
Formulation:
10 mM sodium phosphate, pH 7.5
Nuclease-free:
N
Grade:
GMP
Shipping Temperature:
Ambient
Tested Applications:
Bioassay