Human Recombinant PDGF-AB (from E. coli)

Supplier: VWR International

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Supplier: VWR International

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About this item

Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis. PDGF synthesis is induced by IL-1, IL-6, TNF-α, TGF-β and EGF signaling. PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells. PDGF is also stored in the alpha-granules of platelets and is released upon adherence to traumatized tissues.

High quality
Low endotoxin
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PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-α and PDGFR-β.

Caution: For research use only.

Specifications

Conjugation:
Unconjugated
Protein Function:
Cytokine
Protein/Peptide Type:
Recombinant
Source:
E. coli
Species:
Human
Storage Conditions:
–20 °C
Endotoxin Content:
Endotoxin LAL (EU/µg) ≤0.1
Biological Activity:
Bioactivity assay: NR6R-3T3 cell proliferation; ED50 ≤20 ng/ml; Bioactivity ≥5.0×10⁴ units/mg
Gene ID:
P04085/P01127
Reconstitution Instructions:
Sterile water at 0.1 mg/ml
Endotoxin-free:
N
Carrier-Free:
Y
Protease-free:
N
Animal-Free:
N
Protein Synonyms:
GDGF|ODGF
Protein/Peptide Name:
PDGF-AB
Purity:
≥95%
Molecular Weight:
Alpha:14.4 kDa; Beta: 12.4 kDa; Total 26.8 kDa
Sequence:
Alpha chain: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT Beta chain: MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT
Endotoxin Level:
Low
Formulation:
0.1% Trifluoroacetic Acid (TFA)
Nuclease-free:
N
Grade:
RUO
Shipping Temperature:
RT
Tested Applications:
Bioassay