Cell counting kit-8 (CCK-8)

Water-soluble tetrazole (2-(2-methoxy-4nitrobenzene)-3-(4-nitrobenzene)-5-(2,4-disulfobenzene)-2H-tetrazole monosodium salt ), is a compound similar to MTT. In the presence of an electron coupling reagent, it is reduced by dehydrogenase in the mitochondria to an orange-yellow water-soluble formazan dye. This formazan dye dissolve directly in the medium. The water-soluble tetrazole is bioreduced by intracellular dehydrogenase to produce formazan. The more cells proliferate, the darker the color of the medium; the more cytotoxic, the lighter the color. For the same cell, the number of formazan compounds produced is proportional to the number of living cells, and the color intensity is linearly related to the number of cells. It is the CCK-8 kit developed using this feature to directly analyze cell proliferation and toxicity. The CCK-8 method is widely used and can be used for the activity detection of biologically active factors, the screening of anti-tumor drugs, the determination of cell proliferation, the detection of cytotoxicity, and drug sensitivity and other experiments related to cell viability and proliferation. This kit is easy to use. The kit contains a prepared tube of CCK-8 solution containing water-soluble tetrazole, ready to use, no other preparation steps are required. The detection process does not require additional steps to dissolve formazan. It can be directly detected on a microplate reader using a 96-well plate or a 384-well plate, which is suitable for large-scale high-throughput sample detection.

Storage conditions: CCK-8 solution can be stored for one year under the conditions of dark and 0…5℃; 2 years at –20℃; if you need to use it frequently, please store the reagent at 0…5℃ to prevent the background value from increasing. Do not freeze and thaw repeatedly to interfere with the experimental results. Light and moisture sensitive