RPMI Medium 1640 1X HEPES without L-Glutamine is designed for growth of human leukemia cells in either monolayers or suspension and is useful for a wide variety of suspension and monolayer cultures, as well as for hybridomas.

• Animal-component free

  Chemically defined

  GMP manufacture

RPMI 1640 was developed at the Roswell Park Memorial Institute in 1966 for growing normal and neoplastic peripheral blood-derived leukocytes. Additionally, it has been used to cultivate a wide variety of cells in suspension or loosely attached cells such as hybridoma cells. When supplemented with serum or serum substitutes, RPMI 1640 is the most commonly used basal medium for long-term culture of activated T-lymphocytes.