Alkaline phosphatase enhancer is a ready to use stable solution designed to increase the signal generated by alkaline phosphatase several fold. A single treatment of tissue sections with enhancer before the treatment with substrate/chromogen enables the visualization of difficult to localize antigens.

Alkaline phosphatase, an enzyme derived from bovine intestinal mucosa, is often used as a label for in situ hybridization and immunohistochemistry.

Specimens stained with the substrates/chromogens cannot be dehydrated in ethanol and should be mounted in aqueous based mounting medium such as C/C mount.