Specifications
- Antibody Type:Primary
- Antigen Name:Myosin Light Chain 1
- Antigen Symbol:MYL1
- Clonality:Polyclonal
- Conjugation:Unconjugated
- ELISA:Yes
- Host:Rabbit
- ImmunoChemistry:Yes
- Isotype:IgG
- Reactivity:Human
- Western Blot:Yes
- Size:50 μg
- Gene ID:10627
- Antigen Synonyms:MLCB|MRLC3|MRCL3|HEL-S-24|MLC-2B|MYL2B
- Modification:phospho-Ser20
- Storage Buffer:0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide
- Storage Temperature:Store vial at –20 °C prior to opening. Aliquot contents and freeze at –20 °C or below for extended storage Avoid cycles of freezing and thawing.
- Concentration:1.0 mg/ml
- Immunogen:Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to KLH - KKRPQRAT(pS)NVFAMFD (aa 12-27).
- Tested Applications:ELISA, WB
- Purification:Purified by antigen-affinity chromatography.
- Cat. No.:89361-256
- Supplier no.:GTX22480
Specifications
About this item
Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium levels and myosin phosphorylation, resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. This antibody is specific for the phosphorylated form of myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human and mouse have almost identical sequences. In human the phosphorylation site is pS19, while in mouse the site maps to pS20.
For WB: Use at a dilution of 1:5,000, to give a band at approx. 20 kD. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.This phospho specific polyclonal antibody was tested by ELISA and immunoblotting and was found to be reactive with unstimulated and stimulated cardiac myocytes and the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. For ELISA: Use at a dilution of 1:5,000 - 1:20,000. This product has been assayed against 0.1 mg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Type: Primary
Antigen: MYL1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human