The E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200µg of plasmid DNA in less than 60 minutes

Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple "Bind,Wash,Elute" procedure, thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 50mL of an overnight culture in LB medium will typically produce 200µg of high-copy number plasmid DNA. When working with low-copy number plasmids, the starting culture volume can be increased up to 100mL. High-quality DNA is ready for immediate use in routine molecular biology applications such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.

This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA Midi Columns operate by eliminating time-consuming phenol-chloroform extractions and alcohol precipitations.