Laemmli Loading Buffer, 4X is formulated for use in Laemmli SDS-PAGE, a widely used system for protein electrophoresis

• Premixed for convenience
• 4X concentration allows more volume for protein per lane
• Store at room temperature

Mixing Laemmli Loading Buffer, 4X with samples reduces disulfide bonds and denatures the proteins due to the buffer’s 2-mercaptoethanol and SDS detergent content. The binding of SDS to denatured protein imparts uniform negative charge density so that separation on the gel is based on size. Glycerol is included in the loading buffer as a density agent to ensure the samples sink into the sample wells, while bromophenol blue serves as a tracking dye to monitor electrophoretic progression.