VWR® Laemmli Loading Buffer

Supplier: Avantor

Certificates

Supplier: Avantor

Certificates

About this item

Laemmli Loading Buffer, 4X is formulated for use in Laemmli SDS-PAGE, a widely used system for protein electrophoresis

Premixed for convenience
4X concentration allows more volume for protein per lane
Store at room temperature

Mixing Laemmli Loading Buffer, 4X with samples reduces disulfide bonds and denatures the proteins due to the buffer’s 2-mercaptoethanol and SDS detergent content. The binding of SDS to denatured protein imparts uniform negative charge density so that separation on the gel is based on size. Glycerol is included in the loading buffer as a density agent to ensure the samples sink into the sample wells, while bromophenol blue serves as a tracking dye to monitor electrophoretic progression.

Restricted Products: To process your orders without delay, please provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:

• State issued document with your organization's Federal Tax ID Number
• State issued document with your organization's Resale Tax ID Number
• City or County issued Business License
• State Department of Health Services License
• Any other ID issued by the State that includes the business name & address

* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.