The 96-Well Genomic Plant DNA Kit provides an efficient method for isolating total DNA (genomic, mitochondrial and chloroplast DNA) from plant tissue and cells

The 96-Well Genomic DNA Kit (Plant) provides an efficient method for purifying total DNA (including genomic, mitochondrial and chloroplast DNA) from plant tissue and cells. Samples are disrupted by both grinding in liquid nitrogen and lysis buffer incubation. The lysate is treated with RNase A and then filtered to remove cell debris and salt precipitates. In the presence of the binding buffer coupled with chaotropic salt, the genomic DNA in the lysate binds to each column of the binding plate. The contaminants are removed with a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer or TE. The procedure does not require DNA phenol extraction or alcohol precipitation and can be completed in 1 hour. The purified genomic DNA is ready for use in PCR, Real-time PCR, Southern Blotting and RFLP.

Parameters
Sample : up to 100 mg of fresh or 25 mg of dry plant tissue
Yield : up to 30 μg/well
Format : 96-well plates
Operation time : within 60 minutes

Samples are initially disrupted by grinding in liquid nitrogen, followed by lysate treatment with RNase A. The unique GR Buffer is able to lyse most common plant samples and also samples high in polysaccharides. DNA phenol extraction is not required and the entire procedure can be completed in 1 hour. The purified genomic DNA is ready for use in PCR, Real-time PCR, Southern Blotting, mapping and RFLP. The sample size is up to 100mg of fresh plant tissue or 25mg of dry plant tissue.