NucView® Caspase-3 Substrates are novel fluorescent probes that allow real-time detection of apoptosis by monitoring caspase-3/7 activity in intact cells using confocal microscopy, flow cytometry or microplate readers.

• Real-time apoptosis monitoring: non-interfering with caspase activity unlike other caspase substrates or inhibitor-based assays
• Bi-functional substrates: simultaneous detection of caspase 3/7 activity and visualization apoptotic nuclear morphology
• Rapid, no-wash assay for microscopy or flow cytometry
• Stable for several days for performing time-course assays
• Tolerant of formaldehyde fixation and permeabilization
• For use in adherent or suspension cell cultures
• Storage at 4 °C

NucView® substrates consist of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. Upon entering apoptotic cells, the non-fluorescent substrate is cleaved by caspases-3/7 releasing a high-affinity DNA binding dye which migrates to the nucleus and becomes fluorescent upon binding to DNA.

NucView® 488 Caspase-3 Substrate, is also offered as a 1 mM solution in PBS (phosphate-buffered saline) for use in cells that are sensitive to DMSO toxicity. NucView® 405 and 530 are also available in 1 mM in PBS and trial sizes are available for both PBS and DMSO formulations. Note: In non-DMSO sensitive cell types, adding DMSO during the substrate incubation may enhance NucView staining.

Available in 3 color options:
NucView® 488: Ex/Em (bound to DNA) 500 nm/530 nm, NucView® 405 (bound to DNA): Ex/Em 429 nm/469 nm, NucView® 530 (bound to DNA): Ex/Em 528 nm/563 nm.