Thermo Scientific T7 Cell-Free Expression Vectors (pT7CFE1) are cloning plasmids optimized to use with the 1-Step Human in vitro Protein Expression System for in vitro translation (IVT) of tagged fusion proteins.

• Optimized performance designed to provide the highest yield in the human in vitro translation system
• Many options multiple tag and tag-location options available
• Modular MCS multiple cloning site is maintained across vector family to facilitate subcloning
• Cleavable tags HRV 3C cleavage site available on select vectors

The pT7CFE1 Expression Vectors contain the Encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) element that is critical for high levels of cap-independent protein expression in the Human In Vitro Translation System. Each vector features a highly-compatible multiple cloning site (MCS) to facilitate easy insertion of protein coding sequences into and between vectors. The pT7CFE1 Vector is available with single or tandem affinity tags at the N- or C- terminus to facilitate protein purification and detection. The pT7CFE Vectors are suitable for insertion of cloned genes, cDNAs, ORFs or PCR products for in vitro transcription and translation. Custom cloning services are also available.