Nickel Chelating Resin for Immobilized Metal Affinity Chromatography Purification, G-Biosciences

G-Biosciences' Nickel Chelating Resin is an immobilized metal affinity chromatography (IMAC) resin that was specifically designed to utilize Ni²⁺ for the purification of nickel binding proteins, including 6x histidine tagged proteins.

G-Biosciences' Nickel Chelating Resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked agarose beads. The iminodiacetate binds divalent nickel ion with a capacity of 20-40 μmoles Ni²⁺/mL resin. The protein binding capacity is >50 mg protein per ml resin. We have demonstrated binding of >100 mg of a 50 kDa 6X His tagged proteins to a ml of resin. The spin columns are supplied with a resin bed volume of 0.2, 1 and 3 mL with total column volumes of 1, 8 and 22 mL respectively. Columns can be used as a spin format of gravity flow columns.

G-Biosciences' Immobilized Copper, Cobalt and Zinc Chelating Resins are also available. Cobalt has the highest selectivity followed by Zinc, Nickel then Copper, but has the lowest loading capacity. Copper has the highest loading capacity, followed by Nickel then Zinc. The capacity of nickel is >50 mg per mL. Specific binding/wash and elution buffers are available.

: Accessories included. Additional supplies available.

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