G-Biosciences' amine reactive HOOK™ Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups

G-Biosciences' amine reactive HOOK™ Activated Agarose is designed for the generation of peptide and protein affinity columns, for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offers activated agarose for the coupling of peptides and proteins to agarose through their primary amines.

G-Biosciences' amine reactive HOOK™ Activated agarose is also supplied in a complete kit for the generation of 5 x 2 mL resins. The kit is supplied with all the necessary reagents and columns. Binding capacity: 20 mg protein per mL resin.

The aldehyde groups of the agarose react spontaneously with primary amines, located at the N-terminus of proteins or in lysine residues, to form intermediate Schiff Base complexes. These, in turn, are selectively reduced by reductive amination to form stable amine linkages between the agarose and the ligand.