Pierce™ NE-PER® Nuclear and Cytoplasmic Extraction Reagent Kit, Thermo Scientific

: Invitrogen

Pierce™ NE-PER® Nuclear and Cytoplasmic Extraction Reagent Kit, Thermo Scientific

: Invitrogen

The NE-PER nuclear and cytoplasmic extraction kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours.

Fast - obtain nuclear and cytoplasmic fractions in less than two hours
Proven - the NE-PER Reagent Kit is referenced in more than 950 distinct publications
Scalable - two kit sizes for producing extracts from cells and tissues
Convenient - simple instructions do not require ultracentrifugation over gradients
Compatible - use for downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays

From a sample of two million cells (20µL packed), typical cytoplasmic protein yield is 200 to 500µg and typical nuclear protein yield is 100 to 200µg (at a concentration of 1mg/mL). Typical cross-contamination between cytosolic and nuclear fractions is about 10%. The protein concentration of the nuclear extracts can be manipulated easily by varying the volume of nuclear extraction reagent (NER) used in the extraction without any significant loss in extraction efficiency. Specific extraction of nuclear proteins from cells is central to the success of many gene regulation studies.

: Kit contains Cytoplasmic Extraction Reagent I (CER I), 10 mL; Cytoplasmic Extraction Reagent II (CER II), 0.55 mL; Nuclear Extraction Reagent (NER), 5 mL.

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